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Science 301 (5639): 1547-1550

Copyright © 2003 by the American Association for the Advancement of Science

Orientation of Asymmetric Stem Cell Division by the APC Tumor Suppressor and Centrosome

Yukiko M. Yamashita,1 D. Leanne Jones,1 Margaret T. Fuller1,2*

Abstract: Stem cell self-renewal can be specified by local signals from the surrounding microenvironment, or niche. However, the relation between the niche and the mechanisms that ensure the correct balance between stem cell self-renewal and differentiation is poorly understood. Here, we show that dividing Drosophila male germline stem cells use intracellular mechanisms involving centrosome function and cortically localized Adenomatous Polyposis Coli tumor suppressor protein to orient mitotic spindles perpendicular to the niche, ensuring a reliably asymmetric outcome in which one daughter cell remains in the niche and self-renews stem cell identity, whereas the other, displaced away, initiates differentiation.

1 Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305–5329, USA.
2 Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305–5329, USA.

* To whom correspondence should be addressed. E-mail: fuller{at}cmgm.stanford.edu


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   Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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J. Cell Biol. 186, 219-228
   Abstract »    Full Text »    PDF »
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M. Mitsushima, F. Toyoshima, and E. Nishida (2009)
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   Abstract »    Full Text »    PDF »
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E.L. Davies and M.T. Fuller (2009)
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   Abstract »    PDF »
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P. Karpowicz, S. Willaime-Morawek, L. Balenci, B. DeVeale, T. Inoue, and D. van der Kooy (2009)
J. Neurosci. 29, 3885-3896
   Abstract »    Full Text »    PDF »
Positioning cytokinesis.
S. Oliferenko, T. G. Chew, and M. K. Balasubramanian (2009)
Genes & Dev. 23, 660-674
   Abstract »    Full Text »    PDF »
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N. M. Rusan, K. Akong, and M. Peifer (2008)
J. Cell Biol. 183, 203-212
   Abstract »    Full Text »    PDF »
A critical role for Apc in hematopoietic stem and progenitor cell survival.
Z. Qian, L. Chen, A. A. Fernald, B. O. Williams, and M. M. Le Beau (2008)
J. Exp. Med. 205, 2163-2175
   Abstract »    Full Text »    PDF »
Stem Cell Niche: Microenvironment and Beyond.
J. Zhang and L. Li (2008)
J. Biol. Chem. 283, 9499-9503
   Full Text »    PDF »
Akt regulates centrosome migration and spindle orientation in the early Drosophila melanogaster embryo.
G. J. Buttrick, L. M.A. Beaumont, J. Leitch, C. Yau, J. R. Hughes, and J. G. Wakefield (2008)
J. Cell Biol. 180, 537-548
   Abstract »    Full Text »    PDF »
Asymmetric centrosome behavior and the mechanisms of stem cell division.
Y. M. Yamashita and M. T. Fuller (2008)
J. Cell Biol. 180, 261-266
   Abstract »    Full Text »    PDF »
{beta}-Catenin is a Nek2 substrate involved in centrosome separation.
S. Bahmanyar, D. D. Kaplan, J. G. DeLuca, T. H. Giddings Jr., E. T. O'Toole, M. Winey, E. D. Salmon, P. J. Casey, W. J. Nelson, and A. I.M. Barth (2008)
Genes & Dev. 22, 91-105
   Abstract »    Full Text »    PDF »
Planar Spindle Orientation and Asymmetric Cytokinesis in the Mouse Small Intestine.
E. S. Fleming, M. Zajac, D. M. Moschenross, D. C. Montrose, D. W. Rosenberg, A. E. Cowan, and J. S. Tirnauer (2007)
Journal of Histochemistry & Cytochemistry 55, 1173-1180
   Abstract »    Full Text »    PDF »
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S. DiNardo and R. E. Braun (2007)
Science 317, 1696-1697
   Abstract »    Full Text »    PDF »
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E. P. Lucas and J. W. Raff (2007)
J. Cell Biol. 178, 725-732
   Abstract »    Full Text »    PDF »
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L. N. Nejsum and W. J. Nelson (2007)
J. Cell Biol. 178, 323-335
   Abstract »    Full Text »    PDF »
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D. Dajnowiec, P. J.B. Sabatini, T. C. Van Rossum, J. T.K. Lam, M. Zhang, A. Kapus, and B. L. Langille (2007)
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   Abstract »    Full Text »    PDF »
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T. Xie and L. Li (2007)
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   Abstract »    Full Text »    PDF »
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N. M. Rusan and M. Peifer (2007)
J. Cell Biol. 177, 13-20
   Abstract »    Full Text »    PDF »
The canonical Wnt signalling pathway and its APC partner in colon cancer development.
J. Schneikert and J. Behrens (2007)
Gut 56, 417-425
   Full Text »    PDF »
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A. C. Spradling and Y. Zheng (2007)
Science 315, 469-470
   Abstract »    Full Text »    PDF »
Asymmetric Inheritance of Mother Versus Daughter Centrosome in Stem Cell Division.
Y. M. Yamashita, A. P. Mahowald, J. R. Perlin, and M. T. Fuller (2007)
Science 315, 518-521
   Abstract »    Full Text »    PDF »
Adenomatous Polyposis Coli Control of C-terminal Binding Protein-1 Stability Regulates Expression of Intestinal Retinol Dehydrogenases.
L. D. Nadauld, R. Phelps, B. C. Moore, A. Eisinger, I. T. Sandoval, S. Chidester, P. W. Peterson, E. J. Manos, B. Sklow, R. W. Burt, et al. (2006)
J. Biol. Chem. 281, 37828-37835
   Abstract »    Full Text »    PDF »
Cadherins in development: cell adhesion, sorting, and tissue morphogenesis.
J. M. Halbleib and W. J. Nelson (2006)
Genes & Dev. 20, 3199-3214
   Abstract »    Full Text »    PDF »
A CTX Family Cell Adhesion Molecule, JAM4, Is Expressed in Stem Cell and Progenitor Cell Populations of both Male Germ Cell and Hematopoietic Cell Lineages.
G. Nagamatsu, M. Ohmura, T. Mizukami, I. Hamaguchi, S. Hirabayashi, S. Yoshida, Y. Hata, T. Suda, and K. Ohbo (2006)
Mol. Cell. Biol. 26, 8498-8506
   Abstract »    Full Text »    PDF »
Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis.
T. Krishnamoorthy, X. Chen, J. Govin, W. L. Cheung, J. Dorsey, K. Schindler, E. Winter, C. D. Allis, V. Guacci, S. Khochbin, et al. (2006)
Genes & Dev. 20, 2580-2592
   Abstract »    Full Text »    PDF »
Cellular Analyses of the Mitotic Region in the Caenorhabditis elegans Adult Germ Line.
S. L. Crittenden, K. A. Leonhard, D. T. Byrd, and J. Kimble (2006)
Mol. Biol. Cell 17, 3051-3061
   Abstract »    Full Text »    PDF »
Glial cell line-derived neurotrophic factor maintains a POZ-itive influence on stem cells.
C. Payne and R. E. Braun (2006)
PNAS 103, 9751-9752
   Full Text »    PDF »
Testing hypotheses for the functions of APC family proteins using null and truncation alleles in Drosophila.
B. M. McCartney, M. H. Price, R. L. Webb, M. A. Hayden, L. M. Holot, M. Zhou, A. Bejsovec, and M. Peifer (2006)
Development 133, 2407-2418
   Abstract »    Full Text »    PDF »
The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells.
I. Illa-Bochaca and L. M. Montuenga (2006)
J. Exp. Biol. 209, 2215-2223
   Abstract »    Full Text »    PDF »
The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity.
N. A. Siddall, E. A. McLaughlin, N. L. Marriner, and G. R. Hime (2006)
PNAS 103, 8402-8407
   Abstract »    Full Text »    PDF »
Inactivation of aPKC{lambda} results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex.
F. Imai, S.-i. Hirai, K. Akimoto, H. Koyama, T. Miyata, M. Ogawa, S. Noguchi, T. Sasaoka, T. Noda, and S. Ohno (2006)
Development 133, 1735-1744
   Abstract »    Full Text »    PDF »
Anchorage-Independent Growth of Mouse Male Germline Stem Cells In Vitro.
M. Kanatsu-Shinohara, K. Inoue, J. Lee, H. Miki, N. Ogonuki, S. Toyokuni, A. Ogura, and T. Shinohara (2006)
Biol Reprod 74, 522-529
   Abstract »    Full Text »    PDF »
Postnatal stem cell survival: does the niche, a rare harbor where to resist the ebb tide of differentiation, also provide lineage-specific instructions?.
V. Kindler (2005)
J. Leukoc. Biol. 78, 836-844
   Abstract »    Full Text »    PDF »
APC and EB1 Function Together in Mitosis to Regulate Spindle Dynamics and Chromosome Alignment.
R. A. Green, R. Wollman, and K. B. Kaplan (2005)
Mol. Biol. Cell 16, 4609-4622
   Abstract »    Full Text »    PDF »
Support for the immortal strand hypothesis: neural stem cells partition DNA asymmetrically in vitro.
P. Karpowicz, C. Morshead, A. Kam, E. Jervis, J. Ramunas, V. Cheng, and D. van der Kooy (2005)
J. Cell Biol. 170, 721-732
   Abstract »    Full Text »    PDF »
Efforts to create an artificial testis: culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment.
N. Sofikitis, E. Pappas, A. Kawatani, D. Baltogiannis, D. Loutradis, N. Kanakas, D. Giannakis, F. Dimitriadis, K. Tsoukanelis, I. Georgiou, et al. (2005)
Hum. Reprod. Update 11, 229-259
   Abstract »    Full Text »    PDF »
Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions.
M. Kanatsu-Shinohara, H. Miki, K. Inoue, N. Ogonuki, S. Toyokuni, A. Ogura, and T. Shinohara (2005)
Biol Reprod 72, 985-991
   Abstract »    Full Text »    PDF »
Signaling in stem cell niches: lessons from the Drosophila germline.
Y. M. Yamashita, M. T. Fuller, and D. L. Jones (2005)
J. Cell Sci. 118, 665-672
   Abstract »    Full Text »    PDF »
Cystic Renal Neoplasia Following Conditional Inactivation of Apc in Mouse Renal Tubular Epithelium.
C.-N. Qian, J. Knol, P. Igarashi, F. Lin, U. Zylstra, B. T. Teh, and B. O. Williams (2005)
J. Biol. Chem. 280, 3938-3945
   Abstract »    Full Text »    PDF »
Cortical capture of microtubules and spindle polarity in budding yeast - where's the catch?.
S. M. Huisman and M. Segal (2005)
J. Cell Sci. 118, 463-471
   Abstract »    Full Text »    PDF »

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