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Science 301 (5640): 1728-1731

Copyright © 2003 by the American Association for the Advancement of Science

A Seven-Transmembrane RGS Protein That Modulates Plant Cell Proliferation

Jin-Gui Chen,1 Francis S. Willard,2 Jirong Huang,1 Jiansheng Liang,1* Scott A. Chasse,3 Alan M. Jones,1{dagger} David P. Siderovski2

Abstract: G protein–coupled receptors (GPCRs) at the cell surface activate heterotrimeric G proteins by inducing the G protein alpha (G{alpha}) subunit to exchange guanosine diphosphate for guanosine triphosphate. Regulators of G protein signaling (RGS) proteins accelerate the deactivation of G{alpha} subunits to reduce GPCR signaling. Here we identified an RGS protein (AtRGS1) in Arabidopsis that has a predicted structure similar to a GPCR as well as an RGS box with GTPase accelerating activity. Expression of AtRGS1 complemented the pheromone supersensitivity phenotype of a yeast RGS mutant, sst2{Delta}. Loss of AtRGS1 increased the activity of the Arabidopsis G{alpha} subunit, resulting in increased cell elongation in hypocotyls in darkness and increased cell production in roots grown in light. These findings suggest that AtRGS1 is a critical modulator of plant cell proliferation.

1 Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599–3280, USA.
2 Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599–7365, USA.
3 Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599–7260, USA.

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* Permanent address: College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, People's Republic of China.

{dagger} To whom correspondence should be addressed. E-mail: alan_jones{at}unc.edu

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