Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 302 (5642): 81-84

Copyright © 2003 by the American Association for the Advancement of Science

Loss of an MDR Transporter in Compact Stalks of Maize br2 and Sorghum dw3 Mutants

Dilbag S. Multani,1 Steven P. Briggs,2 Mark A. Chamberlin,1 Joshua J. Blakeslee,3 Angus S. Murphy,3 Gurmukh S. Johal4*

Abstract: Agriculturally advantageous reduction in plant height is usually achieved by blocking the action or production of gibberellins. Here, we describe a different dwarfing mechanism found in maize brachytic2 (br2) mutants characterized by compact lower stalk internodes. The height reduction in these plants results from the loss of a P-glycoprotein that modulates polar auxin transport in the maize stalk. The sorghum ortholog of br2 is dwarf3 (dw3), an unstable mutant of long-standing commercial interest and concern. A direct duplication within the dw3 gene is responsible for its mutant nature and also for its instability, because it facilitates unequal crossing-over at the locus.

1 Pioneer Hi-Bred International, 7250 Northwest 62nd Avenue, Johnston, IA 50131, USA.
2 Diversa Corporation, 4955 Directors Place, San Diego, CA 92121, USA.
3 Department of Horticulture, Purdue University, West Lafayette, IN 47907, USA.
4 Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA.

* To whom correspondence should be addressed. E-mail: gjohal{at}purdue.edu

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fine-Mapping and Identification of a Candidate Gene Underlying the d2 Dwarfing Phenotype in Pearl Millet, Cenchrus americanus (L.) Morrone.
R. K. Parvathaneni, V. Jakkula, F. K. Padi, S. Faure, N. Nagarajappa, A. C. Pontaroli, X. Wu, J. L. Bennetzen, and K. M. Devos (2013)
g3 3, 563-572
   Abstract »    Full Text »    PDF »
Population genomic and genome-wide association studies of agroclimatic traits in sorghum.
G. P. Morris, P. Ramu, S. P. Deshpande, C. T. Hash, T. Shah, H. D. Upadhyaya, O. Riera-Lizarazu, P. J. Brown, C. B. Acharya, S. E. Mitchell, et al. (2013)
PNAS 110, 453-458
   Abstract »    Full Text »    PDF »
High-Throughput Discovery of Mutations in Tef Semi-Dwarfing Genes by Next-Generation Sequencing Analysis.
Q. Zhu, S. M. Smith, M. Ayele, L. Yang, A. Jogi, S. R. Chaluvadi, and J. L. Bennetzen (2012)
Genetics 192, 819-829
   Abstract »    Full Text »    PDF »
Identification of QTLs for eight agronomically important traits using an ultra-high-density map based on SNPs generated from high-throughput sequencing in sorghum under contrasting photoperiods.
G. Zou, G. Zhai, Q. Feng, S. Yan, A. Wang, Q. Zhao, J. Shao, Z. Zhang, J. Zou, B. Han, et al. (2012)
J. Exp. Bot. 63, 5451-5462
   Abstract »    Full Text »    PDF »
The Role of PIN Auxin Efflux Carriers in Polar Auxin Transport and Accumulation and Their Effect on Shaping Maize Development.
C. Forestan and S. Varotto (2012)
Mol Plant 5, 787-798
   Abstract »    Full Text »    PDF »
ATP-Binding Cassette B4, an Auxin-Efflux Transporter, Stably Associates with the Plasma Membrane and Shows Distinctive Intracellular Trafficking from That of PIN-FORMED Proteins.
M. Cho, Z.-W. Lee, and H.-T. Cho (2012)
Plant Physiology 159, 642-654
   Abstract »    Full Text »    PDF »
NPH3- and PGP-like genes are exclusively expressed in the apical tip region essential for blue-light perception and lateral auxin transport in maize coleoptiles.
S. Matsuda, T. Kajizuka, A. Kadota, T. Nishimura, and T. Koshiba (2011)
J. Exp. Bot. 62, 3459-3466
   Abstract »    Full Text »    PDF »
Seven Things We Think We Know about Auxin Transport.
W. A. Peer, J. J. Blakeslee, H. Yang, and A. S. Murphy (2011)
Mol Plant 4, 487-504
   Abstract »    Full Text »    PDF »
Brachytic2/ZmABCB1 functions in IAA export from intercalary meristems.
A. S. Knoller, J. J. Blakeslee, E. L. Richards, W. A. Peer, and A. S. Murphy (2010)
J. Exp. Bot. 61, 3689-3696
   Abstract »    Full Text »    PDF »
Regulation of tillering in sorghum: genotypic effects.
H. K. Kim, D. Luquet, E. van Oosterom, M. Dingkuhn, and G. Hammer (2010)
Ann. Bot. 106, 69-78
   Abstract »    Full Text »    PDF »
Adapting APSIM to model the physiology and genetics of complex adaptive traits in field crops.
G. L. Hammer, E. van Oosterom, G. McLean, S. C. Chapman, I. Broad, P. Harland, and R. C. Muchow (2010)
J. Exp. Bot. 61, 2185-2202
   Abstract »    Full Text »    PDF »
Auxin and Monocot Development.
P. McSteen (2010)
Cold Spring Harb Perspect Biol 2, a001479
   Abstract »    Full Text »    PDF »
Auxin transport routes in plant development.
J. Petrasek and J. Friml (2009)
Development 136, 2675-2688
   Abstract »    Full Text »    PDF »
sparse inflorescence1, barren inflorescence1 and barren stalk1 Promote Cell Elongation in Maize Inflorescence Development.
S. Barazesh, C. Nowbakht, and P. McSteen (2009)
Genetics 182, 403-406
   Abstract »    Full Text »    PDF »
Post-transcriptional regulation of auxin transport proteins: cellular trafficking, protein phosphorylation, protein maturation, ubiquitination, and membrane composition.
B. Titapiwatanakun and A. S. Murphy (2009)
J. Exp. Bot. 60, 1093-1107
   Abstract »    Full Text »    PDF »
Developmental disaster1: A novel mutation causing defects during vegetative and inflorescence development in maize (Zea mays, Poaceae).
K. A. Phillips, A. L. Skirpan, N. J. Kaplinsky, and P. McSteen (2009)
Am. J. Botany 96, 420-430
   Abstract »    Full Text »    PDF »
Interaction of PIN and PGP transport mechanisms in auxin distribution-dependent development.
J. Mravec, M. Kubes, A. Bielach, V. Gaykova, J. Petrasek, P. Skupa, S. Chand, E. Benkova, E. Zazimalova, and J. Friml (2008)
Development 135, 3345-3354
   Abstract »    Full Text »    PDF »
Efficient Mapping of Plant Height Quantitative Trait Loci in a Sorghum Association Population With Introgressed Dwarfing Genes.
P. J. Brown, W. L. Rooney, C. Franks, and S. Kresovich (2008)
Genetics 180, 629-637
   Abstract »    Full Text »    PDF »
Modulation of P-glycoproteins by Auxin Transport Inhibitors Is Mediated by Interaction with Immunophilins.
A. Bailly, V. Sovero, V. Vincenzetti, D. Santelia, D. Bartnik, B. W. Koenig, S. Mancuso, E. Martinoia, and M. Geisler (2008)
J. Biol. Chem. 283, 21817-21826
   Abstract »    Full Text »    PDF »
Mutation of the Rice Narrow leaf1 Gene, Which Encodes a Novel Protein, Affects Vein Patterning and Polar Auxin Transport.
J. Qi, Q. Qian, Q. Bu, S. Li, Q. Chen, J. Sun, W. Liang, Y. Zhou, C. Chu, X. Li, et al. (2008)
Plant Physiology 147, 1947-1959
   Abstract »    Full Text »    PDF »
The Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape Formation.
C. Ringli, L. Bigler, B. M. Kuhn, R.-M. Leiber, A. Diet, D. Santelia, B. Frey, S. Pollmann, and M. Klein (2008)
PLANT CELL 20, 1470-1481
   Abstract »    Full Text »    PDF »
Barren inflorescence1 Functions in Organogenesis During Vegetative and Inflorescence Development in Maize.
S. Barazesh and P. McSteen (2008)
Genetics 179, 389-401
   Abstract »    Full Text »    PDF »
P-Glycoprotein4 Displays Auxin Efflux Transporter-Like Action in Arabidopsis Root Hair Cells and Tobacco Cells.
M. Cho, S. H. Lee, and H.-T. Cho (2007)
PLANT CELL 19, 3930-3943
   Abstract »    Full Text »    PDF »
Maize Brittle stalk2 Encodes a COBRA-Like Protein Expressed in Early Organ Development But Required for Tissue Flexibility at Maturity.
A. Sindhu, T. Langewisch, A. Olek, D. S. Multani, M. C. McCann, W. Vermerris, N. C. Carpita, and G. Johal (2007)
Plant Physiology 145, 1444-1459
   Abstract »    Full Text »    PDF »
The gene MACCHI-BOU 4/ENHANCER OF PINOID encodes a NPH3-like protein and reveals similarities between organogenesis and phototropism at the molecular level.
M. Furutani, T. Kajiwara, T. Kato, B. S. Treml, C. Stockum, R. A. Torres-Ruiz, and M. Tasaka (2007)
Development 134, 3849-3859
   Abstract »    Full Text »    PDF »
Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice.
M. Li, W. Xu, W. Yang, Z. Kong, and Y. Xue (2007)
Plant Physiology 144, 1797-1812
   Abstract »    Full Text »    PDF »
Flowering and determinacy in maize.
E. Bortiri and S. Hake (2007)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Interactions among PIN-FORMED and P-Glycoprotein Auxin Transporters in Arabidopsis.
J. J. Blakeslee, A. Bandyopadhyay, O. R. Lee, J. Mravec, B. Titapiwatanakun, M. Sauer, S. N. Makam, Y. Cheng, R. Bouchard, J. Adamec, et al. (2007)
PLANT CELL 19, 131-147
   Abstract »    Full Text »    PDF »
Reduction of Benzenoid Synthesis in Petunia Flowers Reveals Multiple Pathways to Benzoic Acid and Enhancement in Auxin Transport.
I. Orlova, A. Marshall-Colon, J. Schnepp, B. Wood, M. Varbanova, E. Fridman, J. J. Blakeslee, W. A. Peer, A. S. Murphy, D. Rhodes, et al. (2006)
PLANT CELL 18, 3458-3475
   Abstract »    Full Text »    PDF »
Immunophilin-like TWISTED DWARF1 Modulates Auxin Efflux Activities of Arabidopsis P-glycoproteins.
R. Bouchard, A. Bailly, J. J. Blakeslee, S. C. Oehring, V. Vincenzetti, O. R. Lee, I. Paponov, K. Palme, S. Mancuso, A. S. Murphy, et al. (2006)
J. Biol. Chem. 281, 30603-30612
   Abstract »    Full Text »    PDF »
A Gain-of-Function Mutation in the Arabidopsis Pleiotropic Drug Resistance Transporter PDR9 Confers Resistance to Auxinic Herbicides.
H. Ito and W. M. Gray (2006)
Plant Physiology 142, 63-74
   Abstract »    Full Text »    PDF »
ZmPIN1a and ZmPIN1b Encode Two Novel Putative Candidates for Polar Auxin Transport and Plant Architecture Determination of Maize.
N. Carraro, C. Forestan, S. Canova, J. Traas, and S. Varotto (2006)
Plant Physiology 142, 254-264
   Abstract »    Full Text »    PDF »
Genome-wide analysis of ATP-binding cassette (ABC) proteins in a model legume plant, Lotus japonicus: comparison with Arabidopsis ABC protein family.
A. Sugiyama, N. Shitan, S. Sato, Y. Nakamura, S. Tabata, and K. Yazaki (2006)
DNA Res 13, 205-228
   Abstract »    Full Text »    PDF »
PGP4, an ATP Binding Cassette P-Glycoprotein, Catalyzes Auxin Transport in Arabidopsis thaliana Roots.
K. Terasaka, J. J. Blakeslee, B. Titapiwatanakun, W. A. Peer, A. Bandyopadhyay, S. N. Makam, O. R. Lee, E. L. Richards, A. S. Murphy, F. Sato, et al. (2005)
PLANT CELL 17, 2922-2939
   Abstract »    Full Text »    PDF »
Isolation, Characterization, and Pericycle-Specific Transcriptome Analyses of the Novel Maize Lateral and Seminal Root Initiation Mutant rum1.
K. Woll, L. A. Borsuk, H. Stransky, D. Nettleton, P. S. Schnable, and F. Hochholdinger (2005)
Plant Physiology 139, 1255-1267
   Abstract »    Full Text »    PDF »
NpPDR1, a Pleiotropic Drug Resistance-Type ATP-Binding Cassette Transporter from Nicotiana plumbaginifolia, Plays a Major Role in Plant Pathogen Defense.
Y. Stukkens, A. Bultreys, S. Grec, T. Trombik, D. Vanham, and M. Boutry (2005)
Plant Physiology 139, 341-352
   Abstract »    Full Text »    PDF »
Two Homologous ATP-Binding Cassette Transporter Proteins, AtMDR1 and AtPGP1, Regulate Arabidopsis Photomorphogenesis and Root Development by Mediating Polar Auxin Transport.
R. Lin and H. Wang (2005)
Plant Physiology 138, 949-964
   Abstract »    Full Text »    PDF »
Auxin: Regulation, Action, and Interaction.
A. W. WOODWARD and B. BARTEL (2005)
Ann. Bot. 95, 707-735
   Abstract »    Full Text »    PDF »
Variation in Expression and Protein Localization of the PIN Family of Auxin Efflux Facilitator Proteins in Flavonoid Mutants with Altered Auxin Transport in Arabidopsis thaliana.
W. A. Peer, A. Bandyopadhyay, J. J. Blakeslee, S. N. Makam, R. J. Chen, P. H. Masson, and A. S. Murphy (2004)
PLANT CELL 16, 1898-1911
   Abstract »    Full Text »    PDF »
Relocalization of the PIN1 Auxin Efflux Facilitator Plays a Role in Phototropic Responses.
J. J. Blakeslee, A. Bandyopadhyay, W. A. Peer, S. N. Makam, and A. S. Murphy (2004)
Plant Physiology 134, 28-31
   Full Text »    PDF »
PLANT BIOLOGY: Enhanced: Hormones and the Green Revolution.
F. Salamini (2003)
Science 302, 71-72
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882