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Science 302 (5645): 639-642

Copyright © 2003 by the American Association for the Advancement of Science

The BRCT Domain Is a Phospho-Protein Binding Domain

Xiaochun Yu,1 Claudia Christiano Silva Chini,1 Miao He,2 Georges Mer,3 Junjie Chen1*

Abstract: The carboxyl-terminal domain (BRCT) of the Breast Cancer Gene 1 (BRCA1) protein is an evolutionarily conserved module that exists in a large number of proteins from prokaryotes to eukaryotes. Although most BRCT domain–containing proteins participate in DNA-damage checkpoint or DNA-repair pathways, or both, the function of the BRCT domain is not fully understood. We show that the BRCA1 BRCT domain directly interacts with phosphorylated BRCA1-Associated Carboxyl-terminal Helicase (BACH1). This specific interaction between BRCA1 and phosphorylated BACH1 is cell cycle regulated and is required for DNA damage–induced checkpoint control during the transition from G2 to M phase of the cell cycle. Further, we show that two other BRCT domains interact with their respective physiological partners in a phosphorylation-dependent manner. Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control.

1 Department of Oncology, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
2 Department of Medical Genetics, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
3 Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, MN 55905, USA.

* To whom correspondence should be addressed. E-mail: chen.junjie{at}mayo.edu

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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M. L. Kilkenny, A. S. Dore, S. M. Roe, K. Nestoras, J. C.Y. Ho, F. Z. Watts, and L. H. Pearl (2008)
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   Abstract »    Full Text »    PDF »
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A. De Nicolo, M. Tancredi, G. Lombardi, C. C. Flemma, S. Barbuti, C. Di Cristofano, B. Sobhian, G. Bevilacqua, R. Drapkin, and M. A. Caligo (2008)
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   Abstract »    Full Text »    PDF »
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J. Yan, X.-P. Yang, Y.-S. Kim, and A. M. Jetten (2008)
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   Abstract »    Full Text »    PDF »
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W. Gong, D. Zhou, Y. Ren, Y. Wang, Z. Zuo, Y. Shen, F. Xiao, Q. Zhu, A. Hong, X. Zhou, et al. (2008)
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   Abstract »    Full Text »    PDF »
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B. Wang and S. J. Elledge (2007)
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   Abstract »    Full Text »    PDF »
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K. C. Manthey, S. Opiyo, J. G. Glanzer, D. Dimitrova, J. Elliott, and G. G. Oakley (2007)
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   Abstract »    Full Text »    PDF »
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J. L. Wood, N. Singh, G. Mer, and J. Chen (2007)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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G. Coster, Z. Hayouka, L. Argaman, C. Strauss, A. Friedler, M. Brandeis, and M. Goldberg (2007)
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   Abstract »    Full Text »    PDF »
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K. Brunk, B. Vernay, E. Griffith, N. L. Reynolds, D. Strutt, P. W. Ingham, and A. P. Jackson (2007)
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   Abstract »    Full Text »    PDF »
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E. M. Kass, J. Ahn, T. Tanaka, W. A. Freed-Pastor, S. Keezer, and C. Prives (2007)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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I. M. Munoz, P. A. Jowsey, R. Toth, and J. Rouse (2007)
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   Abstract »    Full Text »    PDF »
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M. Nakanishi, T. Ozaki, H. Yamamoto, T. Hanamoto, H. Kikuchi, K. Furuya, M. Asaka, D. Delia, and A. Nakagawara (2007)
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   Abstract »    Full Text »    PDF »
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X. Hong, L. Lei, B. Kunert, R. Naredla, S. E. Applequist, A. Grandien, and R. Glas (2007)
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   Abstract »    Full Text »    PDF »
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M. Venere, A. Snyder, O. Zgheib, and T. D. Halazonetis (2007)
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   Abstract »    Full Text »    PDF »
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S. Delacroix, J. M. Wagner, M. Kobayashi, K.-i. Yamamoto, and L. M. Karnitz (2007)
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   Abstract »    Full Text »    PDF »
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B. Wang, S. Matsuoka, B. A. Ballif, D. Zhang, A. Smogorzewska, S. P. Gygi, and S. J. Elledge (2007)
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   Abstract »    Full Text »    PDF »
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A. F. Lerch-Gaggl, K. Sun, and S. A. Duncan (2007)
J. Biol. Chem. 282, 11308-11316
   Abstract »    Full Text »    PDF »
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T. Wiltshire, J. Senft, Y. Wang, G. W. Konat, S. L. Wenger, E. Reed, and W. Wang (2007)
Mol. Pharmacol. 71, 1051-1060
   Abstract »    Full Text »    PDF »
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B. P. C. Chen, N. Uematsu, J. Kobayashi, Y. Lerenthal, A. Krempler, H. Yajima, M. Lobrich, Y. Shiloh, and D. J. Chen (2007)
J. Biol. Chem. 282, 6582-6587
   Abstract »    Full Text »    PDF »
Determination of Cancer Risk Associated with Germ Line BRCA1 Missense Variants by Functional Analysis.
M. A. Carvalho, S. M. Marsillac, R. Karchin, S. Manoukian, S. Grist, R. F. Swaby, T. P. Urmenyi, E. Rondinelli, R. Silva, L. Gayol, et al. (2007)
Cancer Res. 67, 1494-1501
   Abstract »    Full Text »    PDF »
Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation.
C. DelloRusso, P. L. Welcsh, W. Wang, R. L. Garcia, M.-C. King, and E. M. Swisher (2007)
Mol. Cancer Res. 5, 35-45
   Abstract »    Full Text »    PDF »
The Tandem BRCT Domain of 53BP1 Is Not Required for Its Repair Function.
I. Ward, J.-E. Kim, K. Minn, C. C. Chini, G. Mer, and J. Chen (2006)
J. Biol. Chem. 281, 38472-38477
   Abstract »    Full Text »    PDF »
BRCA1 ubiquitinates its phosphorylation-dependent binding partner CtIP..
X. Yu, S. Fu, M. Lai, R. Baer, and J. Chen (2006)
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   Abstract »    Full Text »    PDF »
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L.-L. Du, T. M. Nakamura, and P. Russell (2006)
Genes & Dev. 20, 1583-1596
   Abstract »    Full Text »    PDF »
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R. I. Yarden and M. Z. Papa (2006)
Mol. Cancer Ther. 5, 1396-1404
   Abstract »    Full Text »    PDF »
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C. Gabrielse, C. T. Miller, K. H. McConnell, A. DeWard, C. A. Fox, and M. Weinreich (2006)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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H.-S. Kim, H. Li, M. Cevher, A. Parmelee, D. Fonseca, F. E. Kleiman, and S. B. Lee (2006)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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R. D. Kennedy and A. D. D'Andrea (2005)
Genes & Dev. 19, 2925-2940
   Abstract »    Full Text »    PDF »
Fcp1 directly recognizes the C-terminal domain (CTD) and interacts with a site on RNA polymerase II distinct from the CTD.
M.-H. Suh, P. Ye, M. Zhang, S. Hausmann, S. Shuman, A. L. Gnatt, and J. Fu (2005)
PNAS 102, 17314-17319
   Abstract »    Full Text »    PDF »
Inhibition of Aurora-B kinase activity by poly(ADP-ribosyl)ation in response to DNA damage.
L. Monaco, U. Kolthur-Seetharam, R. Loury, J. M.-d. Murcia, G. de Murcia, and P. Sassone-Corsi (2005)
PNAS 102, 14244-14248
   Abstract »    Full Text »    PDF »
Structure of the BRCT Repeat Domain of MDC1 and Its Specificity for the Free COOH-terminal End of the {gamma}-H2AX Histone Tail.
M. S. Lee, R. A. Edwards, G. L. Thede, and J. N. M. Glover (2005)
J. Biol. Chem. 280, 32053-32056
   Abstract »    Full Text »    PDF »

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