Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Logo for

Science 302 (5645): 639-642

Copyright © 2003 by the American Association for the Advancement of Science

The BRCT Domain Is a Phospho-Protein Binding Domain

Xiaochun Yu,1 Claudia Christiano Silva Chini,1 Miao He,2 Georges Mer,3 Junjie Chen1*

Abstract: The carboxyl-terminal domain (BRCT) of the Breast Cancer Gene 1 (BRCA1) protein is an evolutionarily conserved module that exists in a large number of proteins from prokaryotes to eukaryotes. Although most BRCT domain–containing proteins participate in DNA-damage checkpoint or DNA-repair pathways, or both, the function of the BRCT domain is not fully understood. We show that the BRCA1 BRCT domain directly interacts with phosphorylated BRCA1-Associated Carboxyl-terminal Helicase (BACH1). This specific interaction between BRCA1 and phosphorylated BACH1 is cell cycle regulated and is required for DNA damage–induced checkpoint control during the transition from G2 to M phase of the cell cycle. Further, we show that two other BRCT domains interact with their respective physiological partners in a phosphorylation-dependent manner. Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control.

1 Department of Oncology, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
2 Department of Medical Genetics, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
3 Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, MN 55905, USA.

* To whom correspondence should be addressed. E-mail: chen.junjie{at}mayo.edu

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Evidence for a Structural Relationship between BRCT Domains and the Helicase Domains of the Replication Initiators Encoded by the Polyomaviridae and Papillomaviridae Families of DNA Tumor Viruses.
A. Kumar, W. S. Joo, G. Meinke, S. Moine, E. N. Naumova, and P. A. Bullock (2008)
J. Virol. 82, 8849-8862
   Abstract »    Full Text »    PDF »
Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair.
M. L. Kilkenny, A. S. Dore, S. M. Roe, K. Nestoras, J. C.Y. Ho, F. Z. Watts, and L. H. Pearl (2008)
Genes & Dev. 22, 2034-2047
   Abstract »    Full Text »    PDF »
A Novel Breast Cancer-Associated BRIP1 (FANCJ/BACH1) Germ-line Mutation Impairs Protein Stability and Function.
A. De Nicolo, M. Tancredi, G. Lombardi, C. C. Flemma, S. Barbuti, C. Di Cristofano, B. Sobhian, G. Bevilacqua, R. Drapkin, and M. A. Caligo (2008)
Clin. Cancer Res. 14, 4672-4680
   Abstract »    Full Text »    PDF »
RAP80 Responds to DNA Damage Induced by Both Ionizing Radiation and UV Irradiation and Is Phosphorylated at Ser205.
J. Yan, X.-P. Yang, Y.-S. Kim, and A. M. Jetten (2008)
Cancer Res. 68, 4269-4276
   Abstract »    Full Text »    PDF »
PepCyber:P~PEP: a database of human protein protein interactions mediated by phosphoprotein-binding domains.
W. Gong, D. Zhou, Y. Ren, Y. Wang, Z. Zuo, Y. Shen, F. Xiao, Q. Zhu, A. Hong, X. Zhou, et al. (2008)
Nucleic Acids Res. 36, D679-D683
   Abstract »    Full Text »    PDF »
Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage.
B. Wang and S. J. Elledge (2007)
PNAS 104, 20759-20763
   Abstract »    Full Text »    PDF »
NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.
K. C. Manthey, S. Opiyo, J. G. Glanzer, D. Dimitrova, J. Elliott, and G. G. Oakley (2007)
J. Cell Sci. 120, 4221-4229
   Abstract »    Full Text »    PDF »
MCPH1 Functions in an H2AX-dependent but MDC1-independent Pathway in Response to DNA Damage.
J. L. Wood, N. Singh, G. Mer, and J. Chen (2007)
J. Biol. Chem. 282, 35416-35423
   Abstract »    Full Text »    PDF »
Structural Requirements for the BARD1 Tumor Suppressor in Chromosomal Stability and Homology-directed DNA Repair.
M. Laufer, S. V. Nandula, A. P. Modi, S. Wang, M. Jasin, V. V. V. S. Murty, T. Ludwig, and R. Baer (2007)
J. Biol. Chem. 282, 34325-34333
   Abstract »    Full Text »    PDF »
The DNA Damage Response Mediator MDC1 Directly Interacts with the Anaphase-promoting Complex/Cyclosome.
G. Coster, Z. Hayouka, L. Argaman, C. Strauss, A. Friedler, M. Brandeis, and M. Goldberg (2007)
J. Biol. Chem. 282, 32053-32064
   Abstract »    Full Text »    PDF »
Microcephalin coordinates mitosis in the syncytial Drosophila embryo.
K. Brunk, B. Vernay, E. Griffith, N. L. Reynolds, D. Strutt, P. W. Ingham, and A. P. Jackson (2007)
J. Cell Sci. 120, 3578-3588
   Abstract »    Full Text »    PDF »
Stability of Checkpoint Kinase 2 Is Regulated via Phosphorylation at Serine 456.
E. M. Kass, J. Ahn, T. Tanaka, W. A. Freed-Pastor, S. Keezer, and C. Prives (2007)
J. Biol. Chem. 282, 30311-30321
   Abstract »    Full Text »    PDF »
The ePHD protein SPBP interacts with TopBP1 and together they co-operate to stimulate Ets1-mediated transcription.
E. Sjottem, C. Rekdal, G. Svineng, S. S. Johnsen, H. Klenow, R. D. Uglehus, and T. Johansen (2007)
Nucleic Acids Res. 35, 6648-6662
   Abstract »    Full Text »    PDF »
Activation of BRCA1/BRCA2-Associated Helicase BACH1 Is Required for Timely Progression through S Phase.
E. Kumaraswamy and R. Shiekhattar (2007)
Mol. Cell. Biol. 27, 6733-6741
   Abstract »    Full Text »    PDF »
Phospho-epitope binding by the BRCT domains of hPTIP controls multiple aspects of the cellular response to DNA damage.
I. M. Munoz, P. A. Jowsey, R. Toth, and J. Rouse (2007)
Nucleic Acids Res. 35, 5312-5322
   Abstract »    Full Text »    PDF »
NFBD1/MDC1 Associates with p53 and Regulates Its Function at the Crossroad between Cell Survival and Death in Response to DNA Damage.
M. Nakanishi, T. Ozaki, H. Yamamoto, T. Hanamoto, H. Kikuchi, K. Furuya, M. Asaka, D. Delia, and A. Nakagawara (2007)
J. Biol. Chem. 282, 22993-23004
   Abstract »    Full Text »    PDF »
Tripeptidyl-peptidase II Controls DNA Damage Responses and In vivo {gamma}-Irradiation Resistance of Tumors.
X. Hong, L. Lei, B. Kunert, R. Naredla, S. E. Applequist, A. Grandien, and R. Glas (2007)
Cancer Res. 67, 7165-7174
   Abstract »    Full Text »    PDF »
Phosphorylation of ATR-Interacting Protein on Ser239 Mediates an Interaction with Breast-Ovarian Cancer Susceptibility 1 and Checkpoint Function.
M. Venere, A. Snyder, O. Zgheib, and T. D. Halazonetis (2007)
Cancer Res. 67, 6100-6105
   Abstract »    Full Text »    PDF »
The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1.
S. Delacroix, J. M. Wagner, M. Kobayashi, K.-i. Yamamoto, and L. M. Karnitz (2007)
Genes & Dev. 21, 1472-1477
   Abstract »    Full Text »    PDF »
Abraxas and RAP80 Form a BRCA1 Protein Complex Required for the DNA Damage Response.
B. Wang, S. Matsuoka, B. A. Ballif, D. Zhang, A. Smogorzewska, S. P. Gygi, and S. J. Elledge (2007)
Science 316, 1194-1198
   Abstract »    Full Text »    PDF »
Light Chain 1 of Microtubule-associated Protein 1B Can Negatively Regulate the Action of Pes1.
A. F. Lerch-Gaggl, K. Sun, and S. A. Duncan (2007)
J. Biol. Chem. 282, 11308-11316
   Abstract »    Full Text »    PDF »
BRCA1 Contributes to Cell Cycle Arrest and Chemoresistance in Response to the Anticancer Agent Irofulven.
T. Wiltshire, J. Senft, Y. Wang, G. W. Konat, S. L. Wenger, E. Reed, and W. Wang (2007)
Mol. Pharmacol. 71, 1051-1060
   Abstract »    Full Text »    PDF »
Ataxia Telangiectasia Mutated (ATM) Is Essential for DNA-PKcs Phosphorylations at the Thr-2609 Cluster upon DNA Double Strand Break.
B. P. C. Chen, N. Uematsu, J. Kobayashi, Y. Lerenthal, A. Krempler, H. Yajima, M. Lobrich, Y. Shiloh, and D. J. Chen (2007)
J. Biol. Chem. 282, 6582-6587
   Abstract »    Full Text »    PDF »
Determination of Cancer Risk Associated with Germ Line BRCA1 Missense Variants by Functional Analysis.
M. A. Carvalho, S. M. Marsillac, R. Karchin, S. Manoukian, S. Grist, R. F. Swaby, T. P. Urmenyi, E. Rondinelli, R. Silva, L. Gayol, et al. (2007)
Cancer Res. 67, 1494-1501
   Abstract »    Full Text »    PDF »
Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation.
C. DelloRusso, P. L. Welcsh, W. Wang, R. L. Garcia, M.-C. King, and E. M. Swisher (2007)
Mol. Cancer Res. 5, 35-45
   Abstract »    Full Text »    PDF »
The Tandem BRCT Domain of 53BP1 Is Not Required for Its Repair Function.
I. Ward, J.-E. Kim, K. Minn, C. C. Chini, G. Mer, and J. Chen (2006)
J. Biol. Chem. 281, 38472-38477
   Abstract »    Full Text »    PDF »
The phosphorylated C-terminal domain of Xenopus Cut5 directly mediates ATR-dependent activation of Chk1..
Y. Hashimoto, T. Tsujimura, A. Sugino, and H. Takisawa (2006)
Genes Cells 11, 993-1007
   Abstract »    Full Text »    PDF »
BRCA1 ubiquitinates its phosphorylation-dependent binding partner CtIP..
X. Yu, S. Fu, M. Lai, R. Baer, and J. Chen (2006)
Genes & Dev. 20, 1721-1726
   Abstract »    Full Text »    PDF »
Histone modification-dependent and -independent pathways for recruitment of checkpoint protein Crb2 to double-strand breaks..
L.-L. Du, T. M. Nakamura, and P. Russell (2006)
Genes & Dev. 20, 1583-1596
   Abstract »    Full Text »    PDF »
BRCA1 at the crossroad of multiple cellular pathways: approaches for therapeutic interventions..
R. I. Yarden and M. Z. Papa (2006)
Mol. Cancer Ther. 5, 1396-1404
   Abstract »    Full Text »    PDF »
Detection of a tandem BRCT in Nbs1 and Xrs2 with functional implications in the DNA damage response.
E. Becker, V. Meyer, H. Madaoui, and R. Guerois (2006)
Bioinformatics 22, 1289-1292
   Abstract »    Full Text »    PDF »
A Dbf4p BRCA1 C-Terminal-Like Domain Required for the Response to Replication Fork Arrest in Budding Yeast.
C. Gabrielse, C. T. Miller, K. H. McConnell, A. DeWard, C. A. Fox, and M. Weinreich (2006)
Genetics 173, 541-555
   Abstract »    Full Text »    PDF »
Molecular pathogenesis of Fanconi anemia: recent progress.
T. Taniguchi and A. D. D'Andrea (2006)
Blood 107, 4223-4233
   Abstract »    Full Text »    PDF »
BRCA1 Promotes Induction of ssDNA by Ionizing Radiation..
B. P. Schlegel, F. M. Jodelka, and R. Nunez (2006)
Cancer Res. 66, 5181-5189
   Abstract »    Full Text »    PDF »
DNA Damage-Induced BARD1 Phosphorylation Is Critical for the Inhibition of Messenger RNA Processing by BRCA1/BARD1 Complex..
H.-S. Kim, H. Li, M. Cevher, A. Parmelee, D. Fonseca, F. E. Kleiman, and S. B. Lee (2006)
Cancer Res. 66, 4561-4565
   Abstract »    Full Text »    PDF »
Characterization of the DNA Binding and Structural Properties of the BRCT Region of Human Replication Factor C p140 Subunit.
M. Kobayashi, F. Figaroa, N. Meeuwenoord, L. E. T. Jansen, and G. Siegal (2006)
J. Biol. Chem. 281, 4308-4317
   Abstract »    Full Text »    PDF »
BRCA1 DNA-Binding Activity Is Stimulated by BARD1.
A. M. Simons, A. A. Horwitz, L. M. Starita, K. Griffin, R. S. Williams, J.N. M. Glover, and J. D. Parvin (2006)
Cancer Res. 66, 2012-2018
   Abstract »    Full Text »    PDF »
BRCA1 Affects Lipid Synthesis through Its Interaction with Acetyl-CoA Carboxylase.
K. Moreau, E. Dizin, H. Ray, C. Luquain, E. Lefai, F. Foufelle, M. Billaud, G. M. Lenoir, and N. D. Venezia (2006)
J. Biol. Chem. 281, 3172-3181
   Abstract »    Full Text »    PDF »
Multifactorial contributions to an acute DNA damage response by BRCA1/BARD1-containing complexes.
R. A. Greenberg, B. Sobhian, S. Pathania, S. B. Cantor, Y. Nakatani, and D. M. Livingston (2006)
Genes & Dev. 20, 34-46
   Abstract »    Full Text »    PDF »
DNA damage checkpoints in mammals.
H. Niida and M. Nakanishi (2006)
Mutagenesis 21, 3-9
   Abstract »    Full Text »    PDF »
The Fanconi Anemia/BRCA pathway: new faces in the crowd.
R. D. Kennedy and A. D. D'Andrea (2005)
Genes & Dev. 19, 2925-2940
   Abstract »    Full Text »    PDF »
Fcp1 directly recognizes the C-terminal domain (CTD) and interacts with a site on RNA polymerase II distinct from the CTD.
M.-H. Suh, P. Ye, M. Zhang, S. Hausmann, S. Shuman, A. L. Gnatt, and J. Fu (2005)
PNAS 102, 17314-17319
   Abstract »    Full Text »    PDF »
Inhibition of Aurora-B kinase activity by poly(ADP-ribosyl)ation in response to DNA damage.
L. Monaco, U. Kolthur-Seetharam, R. Loury, J. M.-d. Murcia, G. de Murcia, and P. Sassone-Corsi (2005)
PNAS 102, 14244-14248
   Abstract »    Full Text »    PDF »
Structure of the BRCT Repeat Domain of MDC1 and Its Specificity for the Free COOH-terminal End of the {gamma}-H2AX Histone Tail.
M. S. Lee, R. A. Edwards, G. L. Thede, and J. N. M. Glover (2005)
J. Biol. Chem. 280, 32053-32056
   Abstract »    Full Text »    PDF »
Specificity of Protein Interactions Mediated by BRCT Domains of the XRCC1 DNA Repair Protein.
P. T. Beernink, M. Hwang, M. Ramirez, M. B. Murphy, S. A. Doyle, and M. P. Thelen (2005)
J. Biol. Chem. 280, 30206-30213
   Abstract »    Full Text »    PDF »
A Family of Mammalian E3 Ubiquitin Ligases That Contain the UBR Box Motif and Recognize N-Degrons.
T. Tasaki, L. C. F. Mulder, A. Iwamatsu, M. J. Lee, I. V. Davydov, A. Varshavsky, M. Muesing, and Y. T. Kwon (2005)
Mol. Cell. Biol. 25, 7120-7136
   Abstract »    Full Text »    PDF »
Identification of TopBP1 as a c-Abl-interacting Protein and a Repressor for c-Abl Expression.
L. Zeng, Y. Hu, and B. Li (2005)
J. Biol. Chem. 280, 29374-29380
   Abstract »    Full Text »    PDF »
Dynamic assembly and sustained retention of 53BP1 at the sites of DNA damage are controlled by Mdc1/NFBD1.
S. Bekker-Jensen, C. Lukas, F. Melander, J. Bartek, and J. Lukas (2005)
J. Cell Biol. 170, 201-211
   Abstract »    Full Text »    PDF »
Analysis of the DNA Substrate Specificity of the Human BACH1 Helicase Associated with Breast Cancer.
R. Gupta, S. Sharma, J. A. Sommers, Z. Jin, S. B. Cantor, and R. M. Brosh Jr. (2005)
J. Biol. Chem. 280, 25450-25460
   Abstract »    Full Text »    PDF »
Depletion of BRCA1 impairs differentiation but enhances proliferation of mammary epithelial cells.
S. Furuta, X. Jiang, B. Gu, E. Cheng, P.-L. Chen, and W.-H. Lee (2005)
PNAS 102, 9176-9181
   Abstract »    Full Text »    PDF »
A structural perspective of CTD function.
A. Meinhart, T. Kamenski, S. Hoeppner, S. Baumli, and P. Cramer (2005)
Genes & Dev. 19, 1401-1415
   Abstract »    Full Text »    PDF »
53BP1 exchanges slowly at the sites of DNA damage and appears to require RNA for its association with chromatin.
F. Pryde, S. Khalili, K. Robertson, J. Selfridge, A.-M. Ritchie, D. W. Melton, D. Jullien, and Y. Adachi (2005)
J. Cell Sci. 118, 2043-2055
   Abstract »    Full Text »    PDF »
Cell Cycle Dependence of DNA-dependent Protein Kinase Phosphorylation in Response to DNA Double Strand Breaks.
B. P. C. Chen, D. W. Chan, J. Kobayashi, S. Burma, A. Asaithamby, K. Morotomi-Yano, E. Botvinick, J. Qin, and D. J. Chen (2005)
J. Biol. Chem. 280, 14709-14715
   Abstract »    Full Text »    PDF »
The 8-kDa Dynein Light Chain Binds to p53-binding Protein 1 and Mediates DNA Damage-induced p53 Nuclear Accumulation.
K. W.-H. Lo, H.-M. Kan, L.-N. Chan, W.-G. Xu, K.-P. Wang, Z. Wu, M. Sheng, and M. Zhang (2005)
J. Biol. Chem. 280, 8172-8179
   Abstract »    Full Text »    PDF »
Vertebrate DNA damage tolerance requires the C-terminus but not BRCT or transferase domains of REV1.
A.-L. Ross, L. J. Simpson, and J. E. Sale (2005)
Nucleic Acids Res. 33, 1280-1289
   Abstract »    Full Text »    PDF »
Systematic, RNA-Interference-Mediated Identification of mus-101 Modifier Genes in Caenorhabditis elegans.
A. H. Holway, C. Hung, and W. M. Michael (2005)
Genetics 169, 1451-1460
   Abstract »    Full Text »    PDF »
The BRCA1 RING and BRCT Domains Cooperate in Targeting BRCA1 to Ionizing Radiation-induced Nuclear Foci.
W. W. Y. Au and B. R. Henderson (2005)
J. Biol. Chem. 280, 6993-7001
   Abstract »    Full Text »    PDF »
Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis.
F. Oceguera-Yanez, K. Kimura, S. Yasuda, C. Higashida, T. Kitamura, Y. Hiraoka, T. Haraguchi, and S. Narumiya (2005)
J. Cell Biol. 168, 221-232
   Abstract »    Full Text »    PDF »
BRCTx Is a Novel, Highly Conserved RAD18-Interacting Protein.
D. J. Adams, L. van der Weyden, F. V. Gergely, M. J. Arends, B. L. Ng, D. Tannahill, R. Kanaar, A. Markus, B. J. Morris, and A. Bradley (2005)
Mol. Cell. Biol. 25, 779-788
   Abstract »    Full Text »    PDF »
The BRCT domain of mammalian Rev1 is involved in regulating DNA translesion synthesis.
J. G. Jansen, A. Tsaalbi-Shtylik, P. Langerak, F. Calléja, C. M. Meijers, H. Jacobs, and N. de Wind (2005)
Nucleic Acids Res. 33, 356-365
   Abstract »    Full Text »    PDF »
Viral Transport of DNA Damage That Mimics a Stalled Replication Fork.
J. Jurvansuu, K. Raj, A. Stasiak, and P. Beard (2005)
J. Virol. 79, 569-580
   Abstract »    Full Text »    PDF »
A computational method for the analysis and prediction of protein:phosphopeptide-binding sites.
B. A. Joughin, B. Tidor, and M. B. Yaffe (2005)
Protein Sci. 14, 131-139
   Abstract »    Full Text »    PDF »
Functional Analysis of the RING-Type Ubiquitin Ligase Family of Arabidopsis.
S. L. Stone, H. Hauksdottir, A. Troy, J. Herschleb, E. Kraft, and J. Callis (2005)
Plant Physiology 137, 13-30
   Abstract »    Full Text »    PDF »
Human PTIP Facilitates ATM-mediated Activation of p53 and Promotes Cellular Resistance to Ionizing Radiation.
P. A. Jowsey, A. J. Doherty, and J. Rouse (2004)
J. Biol. Chem. 279, 55562-55569
   Abstract »    Full Text »    PDF »
DNA Damage-Induced Cell Cycle Checkpoint Control Requires CtIP, a Phosphorylation-Dependent Binding Partner of BRCA1 C-Terminal Domains.
X. Yu and J. Chen (2004)
Mol. Cell. Biol. 24, 9478-9486
   Abstract »    Full Text »    PDF »
Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases.
H. Koiwa, S. Hausmann, W. Y. Bang, A. Ueda, N. Kondo, A. Hiraguri, T. Fukuhara, J. D. Bahk, D.-J. Yun, R. A. Bressan, et al. (2004)
PNAS 101, 14539-14544
   Abstract »    Full Text »    PDF »
Homo-oligomerization Is the Essential Function of the Tandem BRCT Domains in the Checkpoint Protein Crb2.
L.-L. Du, B. A. Moser, and P. Russell (2004)
J. Biol. Chem. 279, 38409-38414
   Abstract »    Full Text »    PDF »
Phosphorylation and Regulation of DNA Ligase IV Stability by DNA-dependent Protein Kinase.
Y.-G. Wang, C. Nnakwe, W. S. Lane, M. Modesti, and K. M. Frank (2004)
J. Biol. Chem. 279, 37282-37290
   Abstract »    Full Text »    PDF »
BRCA1 Can Modulate RNA Polymerase II Carboxy-Terminal Domain Phosphorylation Levels.
A. Moisan, C. Larochelle, B. Guillemette, and L. Gaudreau (2004)
Mol. Cell. Biol. 24, 6947-6956
   Abstract »    Full Text »    PDF »
Microcephalin Is a DNA Damage Response Protein Involved in Regulation of CHK1 and BRCA1.
X. Xu, J. Lee, and D. F. Stern (2004)
J. Biol. Chem. 279, 34091-34094
   Abstract »    Full Text »    PDF »
Replication Protein A and the Mre11{middle dot}Rad50{middle dot}Nbs1 Complex Co-localize and Interact at Sites of Stalled Replication Forks.
J. G. Robison, J. Elliott, K. Dixon, and G. G. Oakley (2004)
J. Biol. Chem. 279, 34802-34810
   Abstract »    Full Text »    PDF »
Ubiquitination and Proteasomal Degradation of the BRCA1 Tumor Suppressor Is Regulated during Cell Cycle Progression.
A. D. Choudhury, H. Xu, and R. Baer (2004)
J. Biol. Chem. 279, 33909-33918
   Abstract »    Full Text »    PDF »
Histone H2A Phosphorylation Controls Crb2 Recruitment at DNA Breaks, Maintains Checkpoint Arrest, and Influences DNA Repair in Fission Yeast.
T. M. Nakamura, L.-L. Du, C. Redon, and P. Russell (2004)
Mol. Cell. Biol. 24, 6215-6230
   Abstract »    Full Text »    PDF »
BRCA1 Interacts with and Is Required for Paclitaxel-Induced Activation of Mitogen-Activated Protein Kinase Kinase Kinase 3.
P. M. Gilmore, N. McCabe, J. E. Quinn, R. D. Kennedy, J. J. Gorski, H. N. Andrews, S. McWilliams, M. Carty, P. B. Mullan, W. P. Duprex, et al. (2004)
Cancer Res. 64, 4148-4154
   Abstract »    Full Text »    PDF »
Interaction of hREV1 with three human Y-family DNA polymerases.
E. Ohashi, Y. Murakumo, N. Kanjo, J.-i. Akagi, C. Masutani, F. Hanaoka, and H. Ohmori (2004)
Genes Cells 9, 523-531
   Abstract »    Full Text »    PDF »
Chk1 activation requires Rad9 S/TQ-site phosphorylation to promote association with C-terminal BRCT domains of Rad4TOPBP1.
K. Furuya, M. Poitelea, L. Guo, T. Caspari, and A. M. Carr (2004)
Genes & Dev. 18, 1154-1164
   Abstract »    Full Text »    PDF »
Mutational Analysis of Terminal Deoxynucleotidyltransferase- Mediated N-Nucleotide Addition in V(D)J Recombination.
J. A. E. Repasky, E. Corbett, C. Boboila, and D. G. Schatz (2004)
J. Immunol. 172, 5478-5488
   Abstract »    Full Text »    PDF »
Schizosaccharomyces pombe Carboxyl-terminal Domain (CTD) Phosphatase Fcp1: DISTRIBUTIVE MECHANISM, MINIMAL CTD SUBSTRATE, AND ACTIVE SITE MAPPING.
S. Hausmann, H. Erdjument-Bromage, and S. Shuman (2004)
J. Biol. Chem. 279, 10892-10900
   Abstract »    Full Text »    PDF »