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Science 302 (5648): 1208-1212

Copyright © 2003 by the American Association for the Advancement of Science

Control of Nutrient-Sensitive Transcription Programs by the Unconventional Prefoldin URI

Matthias Gstaiger,1* Brian Luke,2 Daniel Hess,1 Edward J. Oakeley,1 Christiane Wirbelauer,1 Marc Blondel,2{dagger} Marc Vigneron,3 Matthias Peter,2 Wilhelm Krek1*{ddagger}

Abstract: Prefoldins (PFDs) are members of a recently identified, small–molecular weight protein family able to assemble into molecular chaperone complexes. Here we describe an unusually large member of this family, termed URI, that forms complexes with other small–molecular weight PFDs and with RPB5, a shared subunit of all three RNA polymerases. Functional analysis of the yeast and human orthologs of URI revealed that both are targets of nutrient signaling and participate in gene expression controlled by the TOR kinase. Thus, URI is a component of a signaling pathway that coordinates nutrient availability with gene expression.

1 Friedrich Miescher Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
2 Eidgenössische Technische Hochschule (ETH)–Hönggerberg, Institute of Biochemistry, CH-8093 Zurich, Switzerland.
3 Unité Mixte de Recherche 7100 CNRS–Université Louis Pasteur, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, Boite postale 10413, 67412 Illkirch Cedex.

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* Present address: ETH-Hönggerberg, Institute of Cell Biology, CH-8093 Zurich, Switzerland.

{dagger} Present address: Cell Cycle Group, Station Biologique du CNRS, Place Georges Teissier, F-29680 Roscoff, France.

{ddagger} To whom correspondence should be addressed. E-mail: wilhelm.krek{at}cell.biol.ethz.ch

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