Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
A Genetic Screen in Drosophila for Metastatic Behavior
Raymond A. Pagliarini and
Tian Xu*
A genetic screen was designed in Drosophila to interrogate itsgenome for mutations sufficient to cause noninvasive tumorsof the eye disc to invade neighboring or distant tissues. Wefound that cooperation between oncogenic RasV12 expression andinactivation of any one of a number of genes affecting cellpolarity leads to metastatic behavior, including basement membranedegradation, loss of E-cadherin expression, migration, invasion,and secondary tumor formation. Inactivation of these cell polaritygenes cannot drive metastatic behavior alone or in combinationwith other tumor-initiating alterations. These findings suggestthat the oncogenic background of tissues makes a distinct contributiontoward metastatic development.
Howard Hughes Medical Institute, Department of Genetics, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.
* To whom correspondence should be addressed. E-mail: tian.xu{at}yale.edu
Palmitoylation of ERBIN is required for its plasma membrane localization..
I. Izawa, M. Nishizawa, Y. Hayashi, and M. Inagaki (2008)
Genes Cells
13, 691-701
|Abstract »|Full Text »|PDF »
hScrib, a human homologue of Drosophila neoplastic tumor suppressor, is a novel death substrate targeted by caspase during the process of apoptosis..
K. Sone, S. Nakagawa, K. Nakagawa, S. Takizawa, Y. Matsumoto, K. Nagasaka, T. Tsuruga, H. Hiraike, O. Hiraike-Wada, Y. Miyamoto, et al. (2008)
Genes Cells
13, 771-785
|Abstract »|Full Text »|PDF »
Basolateral Junctions Utilize Warts Signaling to Control Epithelial-Mesenchymal Transition and Proliferation Crucial For Migration and Invasion of Drosophila Ovarian Epithelial Cells.
M. Zhao, P. Szafranski, C. A. Hall, and S. Goode (2008)
Genetics
178, 1947-1971
|Abstract »|Full Text »|PDF »
Differing Src Signaling Levels Have Distinct Outcomes in Drosophila.
M. Vidal, S. Warner, R. Read, and R. L. Cagan (2007)
Cancer Res.
67, 10278-10285
|Abstract »|Full Text »|PDF »
A Mosaic Genetic Screen for Drosophila Neoplastic Tumor Suppressor Genes Based on Defective Pupation.
L. Menut, T. Vaccari, H. Dionne, J. Hill, G. Wu, and D. Bilder (2007)
Genetics
177, 1667-1677
|Abstract »|Full Text »|PDF »
Suppression of oncogenic properties of c-Myc by LKB1-controlled epithelial organization.
J. I. Partanen, A. I. Nieminen, T. P. Makela, and J. Klefstrom (2007)
PNAS
104, 14694-14699
|Abstract »|Full Text »|PDF »
Basement membrane remodeling is essential for Drosophila disc eversion and tumor invasion.
A. Srivastava, J. C. Pastor-Pareja, T. Igaki, R. Pagliarini, and T. Xu (2007)
PNAS
104, 2721-2726
|Abstract »|Full Text »|PDF »
A Drosophila model of the rhabdomyosarcoma initiator PAX7-FKHR.
R. L. Galindo, J. A. Allport, and E. N. Olson (2006)
PNAS
103, 13439-13444
|Abstract »|Full Text »|PDF »
Characterization of Drosophila mini-me, a Gene Required for Cell Proliferation and Survival.
Mucosal prolapse in the pathogenesis of Peutz-Jeghers polyposis..
M Jansen, W W J de Leng, A F Baas, H Myoshi, L Mathus-Vliegen, M M Taketo, H Clevers, F M Giardiello, and G J A Offerhaus (2006)
Gut
55, 1-5
|Abstract »|Full Text »|PDF »
The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin.
Y. Qin, C. Capaldo, B. M. Gumbiner, and I. G. Macara (2005)
J. Cell Biol.
171, 1061-1071
|Abstract »|Full Text »|PDF »
A hybrid machine-learning approach for segmentation of protein localization data.
P. M. Kasson, J. B. Huppa, M. M. Davis, and A. T. Brunger (2005)
Bioinformatics
21, 3778-3786
|Abstract »|Full Text »|PDF »
Non-cell-autonomous induction of tissue overgrowth by JNK/Ras cooperation in a Drosophila tumor model.
Atypical PKC{iota} contributes to poor prognosis through loss of apical-basal polarity and Cyclin E overexpression in ovarian cancer.
A. M. Eder, X. Sui, D. G. Rosen, L. K Nolden, K. W. Cheng, J. P. Lahad, M. Kango-Singh, K. H. Lu, C. L. Warneke, E. N. Atkinson, et al. (2005)
PNAS
102, 12519-12524
|Abstract »|Full Text »|PDF »
Zebrafish penner/lethal giant larvae 2 functions in hemidesmosome formation, maintenance of cellular morphology and growth regulation in the developing basal epidermis.
M. Sonawane, Y. Carpio, R. Geisler, H. Schwarz, H.-M. Maischein, and C. Nuesslein-Volhard (2005)
Development
132, 3255-3265
|Abstract »|Full Text »|PDF »
The Calpain System Is Involved in the Constitutive Regulation of {beta}-Catenin Signaling Functions.
R. Benetti, T. Copetti, S. Dell'Orso, E. Melloni, C. Brancolini, M. Monte, and C. Schneider (2005)
J. Biol. Chem.
280, 22070-22080
|Abstract »|Full Text »|PDF »
Domains controlling cell polarity and proliferation in the Drosophila tumor suppressor Scribble.
J. Zeitler, C. P. Hsu, H. Dionne, and D. Bilder (2004)
J. Cell Biol.
167, 1137-1146
|Abstract »|Full Text »|PDF »
Epithelial polarity and proliferation control: links from the Drosophila neoplastic tumor suppressors.