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A Genetic Screen in Drosophila for Metastatic Behavior
Raymond A. Pagliarini, and
Tian Xu*
Abstract:
A genetic screen was designed in Drosophila to interrogate itsgenome for mutations sufficient to cause noninvasive tumorsof the eye disc to invade neighboring or distant tissues. Wefound that cooperation between oncogenic RasV12 expression andinactivation of any one of a number of genes affecting cellpolarity leads to metastatic behavior, including basement membranedegradation, loss of E-cadherin expression, migration, invasion,and secondary tumor formation. Inactivation of these cell polaritygenes cannot drive metastatic behavior alone or in combinationwith other tumor-initiating alterations. These findings suggestthat the oncogenic background of tissues makes a distinct contributiontoward metastatic development.
Howard Hughes Medical Institute, Department of Genetics, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.
* To whom correspondence should be addressed. E-mail: tian.xu{at}yale.edu
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