Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 302 (5651): 1775-1779

Copyright © 2003 by the American Association for the Advancement of Science

Regulation of Cell Polarity and Protrusion Formation by Targeting RhoA for Degradation

Hong-Rui Wang,*,1 Yue Zhang,*,1 Barish Ozdamar,1,2 Abiodun A. Ogunjimi,1 Evguenia Alexandrova,3 Gerald H. Thomsen,3 Jeffrey L. Wrana1,2{dagger}

Abstract: The Rho family of small guanosine triphosphatases regulates actin cytoskeleton dynamics that underlie cellular functions such as cell shape changes, migration, and polarity. We found that Smurf1, a HECT domain E3 ubiquitin ligase, regulated cell polarity and protrusive activity and was required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C zeta (PKC{zeta}), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruited Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

1 Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto M56 1x5, Canada.
2 Department of Medical Genetics and Microbiology, University of Toronto, Toronto M5S 1A8, Canada.
3 Department of Biochemistry and Cell Biology, Center for Developmental Genetics, CMM 348, Stony Brook University, Stony Brook, NY 11794–5215, USA.

Back to Top

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: wrana{at}mshri.on.ca


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Tumor-Stroma Interaction: Revealing Fibroblast-Secreted Exosomes as Potent Regulators of Wnt-Planar Cell Polarity Signaling in Cancer Metastasis.
V. Luga and J. L. Wrana (2013)
Cancer Res. 73, 6843-6847
   Abstract »    Full Text »    PDF »
Bazooka inhibits aPKC to limit antagonism of actomyosin networks during amnioserosa apical constriction.
D. J. V. David, Q. Wang, J. J. Feng, and T. J. C. Harris (2013)
Development 140, 4719-4729
   Abstract »    Full Text »    PDF »
The Rho Family Member RhoE Interacts with Skp2 and Is Degraded at the Proteasome during Cell Cycle Progression.
M. Lonjedo, E. Poch, E. Mocholi, M. Hernandez-Sanchez, C. Ivorra, T. F. Franke, R. M. Guasch, and I. Perez-Roger (2013)
J. Biol. Chem. 288, 30872-30882
   Abstract »    Full Text »    PDF »
Smurf1-Mediated Lys29-Linked Nonproteolytic Polyubiquitination of Axin Negatively Regulates Wnt/{beta}-Catenin Signaling.
C. Fei, Z. Li, C. Li, Y. Chen, Z. Chen, X. He, L. Mao, X. Wang, R. Zeng, and L. Li (2013)
Mol. Cell. Biol. 33, 4095-4105
   Abstract »    Full Text »    PDF »
Ubiquitination of Tumor Necrosis Factor Receptor-associated Factor 4 (TRAF4) by Smad Ubiquitination Regulatory Factor 1 (Smurf1) Regulates Motility of Breast Epithelial and Cancer Cells.
X. Wang, C. Jin, Y. Tang, L.-Y. Tang, and Y. E. Zhang (2013)
J. Biol. Chem. 288, 21784-21792
   Abstract »    Full Text »    PDF »
Autophagy Plays a Critical Role in the Degradation of Active RHOA, the Control of Cell Cytokinesis, and Genomic Stability.
A. Belaid, M. Cerezo, A. Chargui, E. Corcelle-Termeau, F. Pedeutour, S. Giuliano, M. Ilie, I. Rubera, M. Tauc, S. Barale, et al. (2013)
Cancer Res. 73, 4311-4322
   Abstract »    Full Text »    PDF »
Atypical Protein Kinase C Phosphorylates Par6 and Facilitates Transforming Growth Factor {beta}-Induced Epithelial-to-Mesenchymal Transition.
A. Gunaratne, B. L. Thai, and G. M. Di Guglielmo (2013)
Mol. Cell. Biol. 33, 874-886
   Abstract »    Full Text »    PDF »
The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1.
Y.-S. Hwang, H.-S. Lee, T. Kamata, K. Mood, H. J. Cho, E. Winterbottom, Y. J. Ji, A. Singh, and I. O. Daar (2013)
Genes & Dev. 27, 491-503
   Abstract »    Full Text »    PDF »
Atypical Protein Kinase C and Par3 Are Required for Proteoglycan-Induced Axon Growth Inhibition.
S.-i. Lee, W. Zhang, M. Ravi, M. Weschenfelder, M. Bastmeyer, and J. M. Levine (2013)
J. Neurosci. 33, 2541-2554
   Abstract »    Full Text »    PDF »
Deubiquitinase FAM/USP9X Interacts with the E3 Ubiquitin Ligase SMURF1 Protein and Protects It from Ligase Activity-dependent Self-degradation.
Y. Xie, M. Avello, M. Schirle, E. McWhinnie, Y. Feng, E. Bric-Furlong, C. Wilson, R. Nathans, J. Zhang, M. W. Kirschner, et al. (2013)
J. Biol. Chem. 288, 2976-2985
   Abstract »    Full Text »    PDF »
Overexpression of CD99 Increases the Migration and Invasiveness of Human Malignant Glioma Cells.
H. J. Seol, J. H. Chang, J. Yamamoto, R. Romagnuolo, Y. Suh, A. Weeks, S. Agnihotri, C. A. Smith, and J. T. Rutka (2013)
Genes & Cancer
   Abstract »    Full Text »    PDF »
CKIP-1 couples Smurf1 ubiquitin ligase with Rpt6 subunit of proteasome to promote substrate degradation.
Y. Wang, J. Nie, Y. Wang, L. Zhang, K. Lu, G. Xing, P. Xie, F. He, and L. Zhang (2012)
EMBO Rep. 13, 1004-1011
   Abstract »    Full Text »    PDF »
The E3 ligase Cdh1-anaphase promoting complex operates upstream of the E3 ligase Smurf1 in the control of axon growth.
M. Kannan, S.-J. Lee, N. Schwedhelm-Domeyer, and J. Stegmuller (2012)
Development 139, 3600-3612
   Abstract »    Full Text »    PDF »
Cyclic AMP regulates formation of mammary epithelial acini in vitro.
P. I. Nedvetsky, S.-H. Kwon, J. Debnath, and K. E. Mostov (2012)
Mol. Biol. Cell 23, 2973-2981
   Abstract »    Full Text »    PDF »
Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts.
M. Nethe, B.-J. de Kreuk, D. V. F. Tauriello, E. C. Anthony, B. Snoek, T. Stumpel, P. C. Salinas, M. M. Maurice, D. Geerts, A. M. Deelder, et al. (2012)
J. Cell Sci. 125, 3430-3442
   Abstract »    Full Text »    PDF »
Signaling Pathways in Cell Polarity.
L. M. McCaffrey and I. G. Macara (2012)
Cold Spring Harb Perspect Biol 4, a009654
   Abstract »    Full Text »    PDF »
Smurf1 Protein Negatively Regulates Interferon-{gamma} Signaling through Promoting STAT1 Protein Ubiquitination and Degradation.
C. Yuan, J. Qi, X. Zhao, and C. Gao (2012)
J. Biol. Chem. 287, 17006-17015
   Abstract »    Full Text »    PDF »
Negative regulation of RhoA translation and signaling by hnRNP-Q1 affects cellular morphogenesis.
L. Xing, X. Yao, K. R. Williams, and G. J. Bassell (2012)
Mol. Biol. Cell 23, 1500-1509
   Abstract »    Full Text »    PDF »
Transcriptional Induction of Salt-inducible Kinase 1 by Transforming Growth Factor {beta} Leads to Negative Regulation of Type I Receptor Signaling in Cooperation with the Smurf2 Ubiquitin Ligase.
P. Lonn, M. Vanlandewijck, E. Raja, M. Kowanetz, Y. Watanabe, K. Kowanetz, E. Vasilaki, C.-H. Heldin, and A. Moustakas (2012)
J. Biol. Chem. 287, 12867-12878
   Abstract »    Full Text »    PDF »
Wnt signalling antagonizes stress granule assembly through a Dishevelled-dependent mechanism.
P. K. Sahoo, P. Murawala, P. T. Sawale, M. R. Sahoo, M. M. Tripathi, S. R. Gaikwad, V. Seshadri, and J. Joseph (2012)
Biology Open 1, 109-119
   Abstract »    Full Text »    PDF »
Rescue of tropomyosin deficiency in Drosophila and human cancer cells by synaptopodin reveals a role of tropomyosin {alpha} in RhoA stabilization.
J. S. Wong, E. Iorns, M. N. Rheault, T. M. Ward, P. Rashmi, U. Weber, M. E. Lippman, C. Faul, M. Mlodzik, and P. Mundel (2012)
EMBO J. 31, 1028-1040
   Abstract »    Full Text »    PDF »
Off the beaten paths: alternative and crosstalk regulation of Rho GTPases.
E. Boulter, S. Estrach, R. Garcia-Mata, and C. C. Feral (2012)
FASEB J 26, 469-479
   Abstract »    Full Text »    PDF »
IAPs regulate the plasticity of cell migration by directly targeting Rac1 for degradation.
T. K. Oberoi, T. Dogan, J. C. Hocking, R.-P. Scholz, J. Mooz, C. L. Anderson, C. Karreman, D. Meyer zu Heringdorf, G. Schmidt, M. Ruonala, et al. (2012)
EMBO J. 31, 14-28
   Abstract »    Full Text »    PDF »
SCFFBXL15 regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1.
Y. Cui, S. He, C. Xing, K. Lu, J. Wang, G. Xing, A. Meng, S. Jia, F. He, and L. Zhang (2011)
EMBO J. 30, 2675-2689
   Abstract »    Full Text »    PDF »
The E3 Ligase Smurf1 Regulates Wolfram Syndrome Protein Stability at the Endoplasmic Reticulum.
X. Guo, S. Shen, S. Song, S. He, Y. Cui, G. Xing, J. Wang, Y. Yin, L. Fan, F. He, et al. (2011)
J. Biol. Chem. 286, 18037-18047
   Abstract »    Full Text »    PDF »
Pivotal Role of the C2 Domain of the Smurf1 Ubiquitin Ligase in Substrate Selection.
K. Lu, P. Li, M. Zhang, G. Xing, X. Li, W. Zhou, M. Bartlam, L. Zhang, Z. Rao, and F. He (2011)
J. Biol. Chem. 286, 16861-16870
   Abstract »    Full Text »    PDF »
Phospholipase C{delta}3 Regulates RhoA/Rho Kinase Signaling and Neurite Outgrowth.
Z. Kouchi, T. Igarashi, N. Shibayama, S. Inanobe, K. Sakurai, H. Yamaguchi, T. Fukuda, S. Yanagi, Y. Nakamura, and K. Fukami (2011)
J. Biol. Chem. 286, 8459-8471
   Abstract »    Full Text »    PDF »
RhoA is dispensable for skin development, but crucial for contraction and directed migration of keratinocytes.
B. Jackson, K. Peyrollier, E. Pedersen, A. Basse, R. Karlsson, Z. Wang, T. Lefever, A. M. Ochsenbein, G. Schmidt, K. Aktories, et al. (2011)
Mol. Biol. Cell 22, 593-605
   Abstract »    Full Text »    PDF »
Genetic Evidence for Antagonism Between Pak Protein Kinase and Rho1 Small GTPase Signaling in Regulation of the Actin Cytoskeleton During Drosophila Oogenesis.
S. Vlachos and N. Harden (2011)
Genetics 187, 501-512
   Abstract »    Full Text »    PDF »
The role of ubiquitylation and degradation in RhoGTPase signalling.
M. Nethe and P. L. Hordijk (2010)
J. Cell Sci. 123, 4011-4018
   Abstract »    Full Text »    PDF »
RhoA Phosphorylation Induces Rac1 Release from Guanine Dissociation Inhibitor {alpha} and Stimulation of Vascular Smooth Muscle Cell Migration.
M. Rolli-Derkinderen, G. Toumaniantz, P. Pacaud, and G. Loirand (2010)
Mol. Cell. Biol. 30, 4786-4796
   Abstract »    Full Text »    PDF »
The Anaphase-Promoting Complex/Cyclosome Activator Cdh1 Modulates Rho GTPase by Targeting p190 RhoGAP for Degradation.
H. Naoe, K. Araki, O. Nagano, Y. Kobayashi, J. Ishizawa, T. Chiyoda, T. Shimizu, K.-i. Yamamura, Y. Sasaki, H. Saya, et al. (2010)
Mol. Cell. Biol. 30, 3994-4005
   Abstract »    Full Text »    PDF »
Conserved role for autophagy in Rho1-mediated cortical remodeling and blood cell recruitment.
P. Kadandale, J. D. Stender, C. K. Glass, and A. A. Kiger (2010)
PNAS 107, 10502-10507
   Abstract »    Full Text »    PDF »
Focal-adhesion targeting links caveolin-1 to a Rac1-degradation pathway.
M. Nethe, E. C. Anthony, M. Fernandez-Borja, R. Dee, D. Geerts, P. J. Hensbergen, A. M. Deelder, G. Schmidt, and P. L. Hordijk (2010)
J. Cell Sci. 123, 1948-1958
   Abstract »    Full Text »    PDF »
The PAR complex regulates pulsed actomyosin contractions during amnioserosa apical constriction in Drosophila.
D. J. V. David, A. Tishkina, and T. J. C. Harris (2010)
Development 137, 1645-1655
   Abstract »    Full Text »    PDF »
PRKC-{zeta} Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention.
S. Yao, A. Bee, D. Brewer, A. Dodson, C. Beesley, Y. Ke, L. Ambroisine, G. Fisher, H. Moller, T. Dickinson, et al. (2010)
Genes & Cancer 1, 444-464
   Abstract »    Full Text »    PDF »
The Ubiquitin Binding Region of the Smurf HECT Domain Facilitates Polyubiquitylation and Binding of Ubiquitylated Substrates.
A. A. Ogunjimi, S. Wiesner, D. J. Briant, X. Varelas, F. Sicheri, J. Forman-Kay, and J. L. Wrana (2010)
J. Biol. Chem. 285, 6308-6315
   Abstract »    Full Text »    PDF »
Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension.
S. J. Warner and G. D. Longmore (2009)
J. Cell Biol. 187, 119-133
   Abstract »    Full Text »    PDF »
Stepwise Maturation of Apicobasal Polarity of the Neuroepithelium Is Essential for Vertebrate Neurulation.
X. Yang, J. Zou, D. R. Hyde, L. A. Davidson, and X. Wei (2009)
J. Neurosci. 29, 11426-11440
   Abstract »    Full Text »    PDF »
Tumor Necrosis Factor-Receptor-associated Factor-4 Is a Positive Regulator of Transforming Growth Factor-{beta} Signaling That Affects Neural Crest Formation.
T. Kalkan, Y. Iwasaki, C. Y. Park, and G. H. Thomsen (2009)
Mol. Biol. Cell 20, 3436-3450
   Abstract »    Full Text »    PDF »
Oncosome Formation in Prostate Cancer: Association with a Region of Frequent Chromosomal Deletion in Metastatic Disease.
D. Di Vizio, J. Kim, M. H. Hager, M. Morello, W. Yang, C. J. Lafargue, L. D. True, M. A. Rubin, R. M. Adam, R. Beroukhim, et al. (2009)
Cancer Res. 69, 5601-5609
   Abstract »    Full Text »    PDF »
Cerebral Cavernous Malformation 2 Protein Promotes Smad Ubiquitin Regulatory Factor 1-mediated RhoA Degradation in Endothelial Cells.
L. E. S. Crose, T. L. Hilder, N. Sciaky, and G. L. Johnson (2009)
J. Biol. Chem. 284, 13301-13305
   Abstract »    Full Text »    PDF »
Protease-dependent versus -independent cancer cell invasion programs: three-dimensional amoeboid movement revisited.
F. Sabeh, R. Shimizu-Hirota, and S. J. Weiss (2009)
J. Cell Biol. 185, 11-19
   Abstract »    Full Text »    PDF »
Smad Ubiquitination Regulatory Factor 2 Promotes Metastasis of Breast Cancer Cells by Enhancing Migration and Invasiveness.
C. Jin, Y.-a. Yang, M. R. Anver, N. Morris, X. Wang, and Y. E. Zhang (2009)
Cancer Res. 69, 735-740
   Abstract »    Full Text »    PDF »
Smurf2 Induces Ubiquitin-dependent Degradation of Smurf1 to Prevent Migration of Breast Cancer Cells.
E. Fukunaga, Y. Inoue, S. Komiya, K. Horiguchi, K. Goto, M. Saitoh, K. Miyazawa, D. Koinuma, A. Hanyu, and T. Imamura (2008)
J. Biol. Chem. 283, 35660-35667
   Abstract »    Full Text »    PDF »
Localized Rho GTPase Activation Regulates RNA Dynamics and Compartmentalization in Tumor Cell Protrusions.
H. C. Stuart, Z. Jia, A. Messenberg, B. Joshi, T. M. Underhill, H. Moukhles, and I. R. Nabi (2008)
J. Biol. Chem. 283, 34785-34795
   Abstract »    Full Text »    PDF »
MicroRNA-155 Is Regulated by the Transforming Growth Factor {beta}/Smad Pathway and Contributes to Epithelial Cell Plasticity by Targeting RhoA.
W. Kong, H. Yang, L. He, J.-j. Zhao, D. Coppola, W. S. Dalton, and J. Q. Cheng (2008)
Mol. Cell. Biol. 28, 6773-6784
   Abstract »    Full Text »    PDF »
Rho5p Is Involved in Mediating the Osmotic Stress Response in Saccharomyces cerevisiae, and Its Activity Is Regulated via Msi1p and Npr1p by Phosphorylation and Ubiquitination.
R. B. Annan, C. Wu, D. D. Waller, M. Whiteway, and D. Y. Thomas (2008)
Eukaryot. Cell 7, 1441-1449
   Abstract »    Full Text »    PDF »
The Rho GDI Rdi1 Regulates Rho GTPases by Distinct Mechanisms.
C. Tiedje, I. Sakwa, U. Just, and T. Hofken (2008)
Mol. Biol. Cell 19, 2885-2896
   Abstract »    Full Text »    PDF »
Transforming Growth Factor-{beta}-stimulated Endocardial Cell Transformation Is Dependent on Par6c Regulation of RhoA.
T. A. Townsend, J. L. Wrana, G. E. Davis, and J. V. Barnett (2008)
J. Biol. Chem. 283, 13834-13841
   Abstract »    Full Text »    PDF »
Smad ubiquitination regulatory factor-2 in the fibrotic kidney: regulation, target specificity, and functional implication.
R. Tan, W. He, X. Lin, L. P. Kiss, and Y. Liu (2008)
Am J Physiol Renal Physiol 294, F1076-F1083
   Abstract »    Full Text »    PDF »
The Synthetic Triterpenoid 2-Cyano-3,12-dioxooleana-1,9-dien-28-oic Acid-Imidazolide Alters Transforming Growth Factor {beta}-dependent Signaling and Cell Migration by Affecting the Cytoskeleton and the Polarity Complex.
C. To, S. Kulkarni, T. Pawson, T. Honda, G. W. Gribble, M. B. Sporn, J. L. Wrana, and G. M. Di Guglielmo (2008)
J. Biol. Chem. 283, 11700-11713
   Abstract »    Full Text »    PDF »
Fbx8 Makes Arf6 Refractory to Function via Ubiquitination.
H. Yano, I. Kobayashi, Y. Onodera, F. Luton, M. Franco, Y. Mazaki, S. Hashimoto, K. Iwai, Z. Ronai, and H. Sabe (2008)
Mol. Biol. Cell 19, 822-832
   Abstract »    Full Text »    PDF »
Instructive role of aPKC{zeta} subcellular localization in the assembly of adherens junctions in neural progenitors.
S. Ghosh, T. Marquardt, J. P. Thaler, N. Carter, S. E. Andrews, S. L. Pfaff, and T. Hunter (2008)
PNAS 105, 335-340
   Abstract »    Full Text »    PDF »
LARG and mDia1 Link G{alpha}12/13 to Cell Polarity and Microtubule Dynamics.
P. Goulimari, H. Knieling, U. Engel, and R. Grosse (2008)
Mol. Biol. Cell 19, 30-40
   Abstract »    Full Text »    PDF »
The Interaction of mPar3 with the Ubiquitin Ligase Smurf2 Is Required for the Establishment of Neuronal Polarity.
J. C. Schwamborn, M. R. Khazaei, and A. W. Puschel (2007)
J. Biol. Chem. 282, 35259-35268
   Abstract »    Full Text »    PDF »
Sheeppox Virus Kelch-Like Gene SPPV-019 Affects Virus Virulence.
C. A. Balinsky, G. Delhon, C. L. Afonso, G. R. Risatti, M. V. Borca, R. A. French, E. R. Tulman, S. J. Geary, and D. L. Rock (2007)
J. Virol. 81, 11392-11401
   Abstract »    Full Text »    PDF »
Increased Expression of the E3 Ubiquitin Ligase RNF5 Is Associated with Decreased Survival in Breast Cancer.
K. D. Bromberg, H. M. Kluger, A. Delaunay, S. Abbas, K. A. DiVito, S. Krajewski, and Z. Ronai (2007)
Cancer Res. 67, 8172-8179
   Abstract »    Full Text »    PDF »
Protein Kinase C{zeta} and Glycogen Synthase Kinase-3{beta} Control Neuronal Polarity in Developing Rodent Enteric Neurons, whereas SMAD Specific E3 Ubiquitin Protein Ligase 1 Promotes Neurite Growth But Does Not Influence Polarity.
B. P. S. Vohra, M. Fu, and R. O. Heuckeroth (2007)
J. Neurosci. 27, 9458-9468
   Abstract »    Full Text »    PDF »
A Novel Regulatory Mechanism of the Bone Morphogenetic Protein (BMP) Signaling Pathway Involving the Carboxyl-Terminal Tail Domain of BMP Type II Receptor.
M. C. Chan, P. H. Nguyen, B. N. Davis, N. Ohoka, H. Hayashi, K. Du, G. Lagna, and A. Hata (2007)
Mol. Cell. Biol. 27, 5776-5789
   Abstract »    Full Text »    PDF »
The NHERF1 PDZ2 Domain Regulates PKA-RhoA-p38-mediated NHE1 Activation and Invasion in Breast Tumor Cells.
R. A. Cardone, A. Bellizzi, G. Busco, E. J. Weinman, M. E. Dell'Aquila, V. Casavola, A. Azzariti, A. Mangia, A. Paradiso, and S. J. Reshkin (2007)
Mol. Biol. Cell 18, 1768-1780
   Abstract »    Full Text »    PDF »
The Myosin IXb Motor Activity Targets the Myosin IXb RhoGAP Domain as Cargo to Sites of Actin Polymerization.
F. van den Boom, H. Dussmann, K. Uhlenbrock, M. Abouhamed, and M. Bahler (2007)
Mol. Biol. Cell 18, 1507-1518
   Abstract »    Full Text »    PDF »
Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity.
J. C. Schwamborn, M. Muller, A. H. Becker, and A. W. Puschel (2007)
EMBO J. 26, 1410-1422
   Abstract »    Full Text »    PDF »
Transglutaminase-dependent RhoA Activation and Depletion by Serotonin in Vascular Smooth Muscle Cells.
C. Guilluy, M. Rolli-Derkinderen, P.-L. Tharaux, G. Melino, P. Pacaud, and G. Loirand (2007)
J. Biol. Chem. 282, 2918-2928
   Abstract »    Full Text »    PDF »
Smurf1 regulates tumor cell plasticity and motility through degradation of RhoA leading to localized inhibition of contractility.
E. Sahai, R. Garcia-Medina, J. Pouyssegur, and E. Vial (2007)
J. Cell Biol. 176, 35-42
   Abstract »    Full Text »    PDF »
Defective Chemokine-Directed Lymphocyte Migration and Development in the Absence of Rho Guanosine Diphosphate-Dissociation Inhibitors {alpha} and {beta}.
H. Ishizaki, A. Togawa, M. Tanaka-Okamoto, K. Hori, M. Nishimura, A. Hamaguchi, T. Imai, Y. Takai, and J. Miyoshi (2006)
J. Immunol. 177, 8512-8521
   Abstract »    Full Text »    PDF »
The Cdc34/SCF Ubiquitination Complex Mediates Saccharomyces cerevisiae Cell Wall Integrity.
X. Varelas, D. Stuart, M. J. Ellison, and C. Ptak (2006)
Genetics 174, 1825-1839
   Abstract »    Full Text »    PDF »
Roles of the Ubiquitin/Proteasome Pathway in Pollen Tube Growth with Emphasis on MG132-Induced Alterations in Ultrastructure, Cytoskeleton, and Cell Wall Components.
X. Sheng, Z. Hu, H. Lu, X. Wang, F. Baluska, J. Samaj, and J. Lin (2006)
Plant Physiology 141, 1578-1590
   Abstract »    Full Text »    PDF »
Requirement of dendritic Akt degradation by the ubiquitin-proteasome system for neuronal polarity.
D. Yan, L. Guo, and Y. Wang (2006)
J. Cell Biol. 174, 415-424
   Abstract »    Full Text »    PDF »
LIM Mineralization Protein-1 Potentiates Bone Morphogenetic Protein Responsiveness via a Novel Interaction with Smurf1 Resulting in Decreased Ubiquitination of Smads.
S. Sangadala, S. D. Boden, M. Viggeswarapu, Y. Liu, and L. Titus (2006)
J. Biol. Chem. 281, 17212-17219
   Abstract »    Full Text »    PDF »
CNF1-induced Ubiquitylation and Proteasome Destruction of Activated RhoA Is Impaired in Smurf1-/- Cells.
L. Boyer, L. Turchi, B. Desnues, A. Doye, G. Ponzio, J.-L. Mege, M. Yamashita, Y. E. Zhang, J. Bertoglio, G. Flatau, et al. (2006)
Mol. Biol. Cell 17, 2489-2497
   Abstract »    Full Text »    PDF »
PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR.
K. Nagano, B. C. Bornhauser, G. Warnasuriya, A. Entwistle, R. Cramer, D. Lindholm, and S. Naaby-Hansen (2006)
EMBO J. 25, 1871-1882
   Abstract »    Full Text »    PDF »
Proteasome-mediated Degradation of Rac1-GTP during Epithelial Cell Scattering.
E. A. Lynch, J. Stall, G. Schmidt, P. Chavrier, and C. D'Souza-Schorey (2006)
Mol. Biol. Cell 17, 2236-2242
   Abstract »    Full Text »    PDF »
Role of Ubiquitylation in Cellular Membrane Transport.
O. Staub and D. Rotin (2006)
Physiol Rev 86, 669-707
   Abstract »    Full Text »    PDF »
The PAR-aPKC system: lessons in polarity.
A. Suzuki and S. Ohno (2006)
J. Cell Sci. 119, 979-987
   Abstract »    Full Text »    PDF »
Tumor Necrosis Factor Promotes Runx2 Degradation through Up-regulation of Smurf1 and Smurf2 in Osteoblasts.
H. Kaneki, R. Guo, D. Chen, Z. Yao, E. M. Schwarz, Y. E. Zhang, B. F. Boyce, and L. Xing (2006)
J. Biol. Chem. 281, 4326-4333
   Abstract »    Full Text »    PDF »
ARAP3 is essential for formation of lamellipodia after growth factor stimulation.
S. Krugmann, S. Andrews, L. Stephens, and P. T. Hawkins (2006)
J. Cell Sci. 119, 425-432
   Abstract »    Full Text »    PDF »
G{alpha}12/13 Is Essential for Directed Cell Migration and Localized Rho-Dia1 Function.
P. Goulimari, T. M. Kitzing, H. Knieling, D. T. Brandt, S. Offermanns, and R. Grosse (2005)
J. Biol. Chem. 280, 42242-42251
   Abstract »    Full Text »    PDF »
Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling.
K. Kurokawa and M. Matsuda (2005)
Mol. Biol. Cell 16, 4294-4303
   Abstract »    Full Text »    PDF »
Tumor Cell Pseudopodial Protrusions: LOCALIZED SIGNALING DOMAINS COORDINATING CYTOSKELETON REMODELING, CELL ADHESION, GLYCOLYSIS, RNA TRANSLOCATION, AND PROTEIN TRANSLATION.
Z. Jia, L. Barbier, H. Stuart, M. Amraei, S. Pelech, J. W. Dennis, P. Metalnikov, P. O'Donnell, and I. R. Nabi (2005)
J. Biol. Chem. 280, 30564-30573
   Abstract »    Full Text »    PDF »
Protein Kinase A Gating of a Pseudopodial-located RhoA/ROCK/p38/NHE1 Signal Module Regulates Invasion in Breast Cancer Cell Lines.
R. A. Cardone, A. Bagorda, A. Bellizzi, G. Busco, L. Guerra, A. Paradiso, V. Casavola, M. Zaccolo, and S. J. Reshkin (2005)
Mol. Biol. Cell 16, 3117-3127
   Abstract »    Full Text »    PDF »
Degradation of the Tumor Suppressor Smad4 by WW and HECT Domain Ubiquitin Ligases.
A. Moren, T. Imamura, K. Miyazono, C.-H. Heldin, and A. Moustakas (2005)
J. Biol. Chem. 280, 22115-22123
   Abstract »    Full Text »    PDF »
Phosphorylation of Serine 188 Protects RhoA from Ubiquitin/Proteasome-Mediated Degradation in Vascular Smooth Muscle Cells.
M. Rolli-Derkinderen, V. Sauzeau, L. Boyer, E. Lemichez, C. Baron, D. Henrion, G. Loirand, and P. Pacaud (2005)
Circ. Res. 96, 1152-1160
   Abstract »    Full Text »    PDF »
Raf-1 regulates Rho signaling and cell migration.
K. Ehrenreiter, D. Piazzolla, V. Velamoor, I. Sobczak, J. V. Small, J. Takeda, T. Leung, and M. Baccarini (2005)
J. Cell Biol. 168, 955-964
   Abstract »    Full Text »    PDF »
Regulation of the Polarity Protein Par6 by TGF{beta} Receptors Controls Epithelial Cell Plasticity.
B. Ozdamar, R. Bose, M. Barrios-Rodiles, H.-R. Wang, Y. Zhang, and J. L. Wrana (2005)
Science 307, 1603-1609
   Abstract »    Full Text »    PDF »
Protein interaction mapping: A Drosophila case study.
E. Formstecher, S. Aresta, V. Collura, A. Hamburger, A. Meil, A. Trehin, C. Reverdy, V. Betin, S. Maire, C. Brun, et al. (2005)
Genome Res. 15, 376-384
   Abstract »    Full Text »    PDF »
Cdc42 Mediates Nucleus Movement and MTOC Polarization in Swiss 3T3 Fibroblasts under Mechanical Shear Stress.
J. S.H. Lee, M. I. Chang, Y. Tseng, and D. Wirtz (2005)
Mol. Biol. Cell 16, 871-880
   Abstract »    Full Text »    PDF »
R-Ras Controls Membrane Protrusion and Cell Migration through the Spatial Regulation of Rac and Rho.
M. A. Wozniak, L. Kwong, D. Chodniewicz, R. L. Klemke, and P. J. Keely (2005)
Mol. Biol. Cell 16, 84-96
   Abstract »    Full Text »    PDF »
GEFT, A Rho Family Guanine Nucleotide Exchange Factor, Regulates Neurite Outgrowth and Dendritic Spine Formation.
B. Bryan, V. Kumar, L. J. Stafford, Y. Cai, G. Wu, and M. Liu (2004)
J. Biol. Chem. 279, 45824-45832
   Abstract »    Full Text »    PDF »
The Rac1 C-terminal Polybasic Region Regulates the Nuclear Localization and Protein Degradation of Rac1.
C. C. Lanning, J. L. Daddona, R. Ruiz-Velasco, S. H. Shafer, and C. L. Williams (2004)
J. Biol. Chem. 279, 44197-44210
   Abstract »    Full Text »    PDF »
Epithelial cell spreading induced by hepatocyte growth factor influences paxillin protein synthesis and posttranslational modification.
A. M. Hopkins, M. Bruewer, G. T. Brown, A. A. Pineda, J. J. Ha, L. M. Winfree, S. V. Walsh, B. A. Babbin, and A. Nusrat (2004)
Am J Physiol Gastrointest Liver Physiol 287, G886-G898
   Abstract »    Full Text »    PDF »
Targeted Deletion of Protein Kinase C {lambda} Reveals a Distribution of Functions between the Two Atypical Protein Kinase C Isoforms.
R. S. Soloff, C. Katayama, M. Y. Lin, J. R. Feramisco, and S. M. Hedrick (2004)
J. Immunol. 173, 3250-3260
   Abstract »    Full Text »    PDF »
CELL BIOLOGY: Smurfing at the Leading Edge.
A. B. Jaffe and A. Hall (2003)
Science 302, 1690-1691
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882