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Science 303 (5659): 836-839

Copyright © 2004 by the American Association for the Advancement of Science

Localized Stabilization of Microtubules by Integrin- and FAK-Facilitated Rho Signaling

Alexander F. Palazzo,1 Christina H. Eng,1,2 David D. Schlaepfer,4 Eugene E. Marcantonio,1,3 Gregg G. Gundersen1,3*

Abstract: Microtubule (MT) stabilization is regulated by the small guanosine triphosphate (GTP)–binding protein Rho and its effector, mammalian homolog of Diaphanous (mDia), in migrating cells, but factors responsible for localized stabilization at the leading edge are unknown. We report that integrin-mediated activation of focal adhesion kinase (FAK) at the leading edge is required for MT stabilization by the Rho-mDia signaling pathway in mouse fibroblasts. MT stabilization also involved FAK-regulated localization of a lipid raft marker, ganglioside GM1, to the leading edge. The integrin-FAK signaling pathway may facilitate Rho-mDia signaling through GM1, or through a specialized membrane domain containing GM1, to stabilize MTs in the leading edge of migrating cells.

1 Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA.
2 Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University, New York, NY 10032, USA.
3 Department of Pathology, Columbia University, New York, NY 10032, USA.
4 Department of Immunology, Scripps Research Institute, La Jolla, CA 92307, USA.

* To whom correspondence should be addressed. E-mail: ggg1{at}columbia.edu

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