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Science 303 (5661): 1179-1181

Copyright © 2004 by the American Association for the Advancement of Science

Regulation of Cell Migration by the C2 Domain of the Tumor Suppressor PTEN

Myrto Raftopoulou, Sandrine Etienne-Manneville, Annette Self, Sarah Nicholls, Alan Hall*

Abstract: PTEN is a tumor suppressor protein that dephosphorylates phosphatidylinositol 3,4,5 trisphosphate and antagonizes the phosphatidylinositol-3 kinase signaling pathway. We show here that PTEN can also inhibit cell migration through its C2 domain, independent of its lipid phosphatase activity. This activity depends on the protein phosphatase activity of PTEN and on dephosphorylation at a single residue, threonine383. The ability of PTEN to control cell migration through its C2 domain is likely to be an important feature of its tumor suppressor activity.

Medical Research Council Laboratory for Molecular Cell Biology and Cell Biology Unit, Cancer Research UK Oncogene and Signal Transduction Group, and Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK.

* To whom correspondence should be addressed. E-mail: alan.hall{at}ucl.ac.uk

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