Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 304 (5668): 286-289

Copyright © 2004 by the American Association for the Advancement of Science

Tip20p Prohibits Back-Fusion of COPII Vesicles with the Endoplasmic Reticulum

Faustin Kamena, and Anne Spang*

Abstract: Directionality in intracellular trafficking is essential to ensure the correct localization of proteins along the secretory pathway. Here, we found evidence for an active mechanism that prohibited back-fusion of de novo–generated vesicles with their donor compartment. Tip20p is a peripheral membrane protein implicated in consumption of COPI vesicles at the endoplasmic reticulum. However, a specific mutant of TIP20 did not interfere with COPII vesicle generation but allowed these vesicles to fuse back to the endoplasmic reticulum, a process that does not occur normally in the cell.

Friedrich Miescher Laboratorium der Max Planck Gesellschaft, Spemannstrasse 39, D-72076 Tübingen, Germany.

* To whom correspondence should be addressed. E-mail: anne.spang{at}tuebingen.mpg.de

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Retrograde Traffic from the Golgi to the Endoplasmic Reticulum.
A. Spang (2013)
Cold Spring Harb Perspect Biol 5, a013391
   Abstract »    Full Text »    PDF »
Secretory Protein Biogenesis and Traffic in the Early Secretory Pathway.
C. K. Barlowe and E. A. Miller (2013)
Genetics 193, 383-410
   Abstract »    Full Text »    PDF »
The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae.
M. Diefenbacher, H. Thorsteinsdottir, and A. Spang (2011)
J. Biol. Chem. 286, 25027-25038
   Abstract »    Full Text »    PDF »
What Is Moving in the Secretory Pathway of Plants?.
E. Rojo and J. Denecke (2008)
Plant Physiology 147, 1493-1503
   Full Text »    PDF »
Ypt1p is essential for retrograde Golgi-ER transport and for Golgi maintenance in S. cerevisiae.
F. Kamena, M. Diefenbacher, C. Kilchert, H. Schwarz, and A. Spang (2008)
J. Cell Sci. 121, 1293-1302
   Abstract »    Full Text »    PDF »
RINT-1 Regulates the Localization and Entry of ZW10 to the Syntaxin 18 Complex.
K. Arasaki, M. Taniguchi, K. Tani, and M. Tagaya (2006)
Mol. Biol. Cell 17, 2780-2788
   Abstract »    Full Text »    PDF »
Dsl1p, Tip20p, and the Novel Dsl3(Sec39) Protein Are Required for the Stability of the Q/t-SNARE Complex at the Endoplasmic Reticulum in Yeast.
B. A. Kraynack, A. Chan, E. Rosenthal, M. Essid, B. Umansky, M. G. Waters, and H. D. Schmitt (2005)
Mol. Biol. Cell 16, 3963-3977
   Abstract »    Full Text »    PDF »
Generation of nonidentical compartments in vesicular transport systems.
R. Heinrich and T. A. Rapoport (2005)
J. Cell Biol. 168, 271-280
   Abstract »    Full Text »    PDF »
Get out! And don't come back!.
W. A. Wells (2004)
J. Cell Biol. 165, 165
   Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882