Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 304 (5673): 1010-1014

Copyright © 2004 by the American Association for the Advancement of Science

TAF1 Activates Transcription by Phosphorylation of Serine 33 in Histone H2B

Tobias Maile,1* Simona Kwoczynski,2*{dagger} Rebeccah J. Katzenberger,3 David A. Wassarman,3{ddagger} Frank Sauer1,2{ddagger}

Abstract: Dynamic changes in chromatin structure, induced by posttranslational modification of histones, play a fundamental role in regulating eukaryotic transcription. Here we report that histone H2B is phosphorylated at evolutionarily conserved Ser33 (H2B-S33) by the carboxyl-terminal kinase domain (CTK) of the Drosophila TFIID subunit TAF1. Phosphorylation of H2B-S33 at the promoter of the cell cycle regulatory gene string and the segmentation gene giant coincides with transcriptional activation. Elimination of TAF1 CTK activity in Drosophila cells and embryos reduces transcriptional activation and phosphorylation of H2B-S33. These data reveal that H2B-S33 is a physiological substrate for the TAF1 CTK and that H2B-S33 phosphorylation is essential for transcriptional activation events that promote cell cycle progression and development.

1 Department of Biochemistry, University of California–Riverside, Riverside, CA 95121, USA.
2 Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
3 Department of Pharmacology, University of Wisconsin (UW)–Madison, 1300 University Avenue, Madison, WI 53706, USA.

Back to Top

* These authors contributed equally to this work.

{dagger} Present address: Heidelberger Institut für Pflanzenwis-senschaften, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.

{ddagger} To whom correspondence should be addressed. E-mail: frank.sauer{at}ucr.edu (F.S.), dawassarman{at}wisc.edu (D.A.W.)

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Phosphorylation-Dependent Regulation of Cyclin D1 and Cyclin A Gene Transcription by TFIID Subunits TAF1 and TAF7.
S. L. Kloet, J. L. Whiting, P. Gafken, J. Ranish, and E. H. Wang (2012)
Mol. Cell. Biol. 32, 3358-3369
   Abstract »    Full Text »    PDF »
The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility.
K. Leser, S. Awe, B. Barckmann, R. Renkawitz-Pohl, and C. Rathke (2012)
Biology Open 1, 597-606
   Abstract »    Full Text »    PDF »
Developmental regulation of N-terminal H2B methylation in Drosophila melanogaster.
A. Villar-Garea, I. Forne, I. Vetter, E. Kremmer, A. Thomae, and A. Imhof (2012)
Nucleic Acids Res. 40, 1536-1549
   Abstract »    Full Text »    PDF »
A Peek into the Complex Realm of Histone Phosphorylation.
T. Banerjee and D. Chakravarti (2011)
Mol. Cell. Biol. 31, 4858-4873
   Abstract »    Full Text »    PDF »
Phosphorylation of Histone H2B Serine 32 Is Linked to Cell Transformation.
A. T. Y. Lau, S.-Y. Lee, Y.-M. Xu, D. Zheng, Y.-Y. Cho, F. Zhu, H.-G. Kim, S.-Q. Li, Z. Zhang, A. M. Bode, et al. (2011)
J. Biol. Chem. 286, 26628-26637
   Abstract »    Full Text »    PDF »
{beta}-N-acetylglucosamine (O-GlcNAc) is part of the histone code.
K. Sakabe, Z. Wang, and G. W. Hart (2010)
PNAS 107, 19915-19920
   Abstract »    Full Text »    PDF »
Transforming Growth Factor-{beta} Regulates Basal Transcriptional Regulatory Machinery to Control Cell Proliferation and Differentiation in Cranial Neural Crest-derived Osteoprogenitor Cells.
J.-i. Iwata, R. Hosokawa, P. A. Sanchez-Lara, M. Urata, H. Slavkin, and Y. Chai (2010)
J. Biol. Chem. 285, 4975-4982
   Abstract »    Full Text »    PDF »
ATM and ATR Pathways Signal Alternative Splicing of Drosophila TAF1 Pre-mRNA in Response to DNA Damage.
R. J. Katzenberger, M. S. Marengo, and D. A. Wassarman (2006)
Mol. Cell. Biol. 26, 9256-9267
   Abstract »    Full Text »    PDF »
Noncoding RNAs of Trithorax Response Elements Recruit Drosophila Ash1 to Ultrabithorax..
T. Sanchez-Elsner, D. Gou, E. Kremmer, and F. Sauer (2006)
Science 311, 1118-1123
   Abstract »    Full Text »    PDF »
Small CTD Phosphatases Function in Silencing Neuronal Gene Expression.
M. Yeo, S.-K. Lee, B. Lee, E. C. Ruiz, S. L. Pfaff, and G. N. Gill (2005)
Science 307, 596-600
   Abstract »    Full Text »    PDF »
Molecular evolution of dinoflagellate luciferases, enzymes with three catalytic domains in a single polypeptide.
L. Liu, T. Wilson, and J. W. Hastings (2004)
PNAS 101, 16555-16560
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882