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Science 306 (5693): 114-117

Copyright © 2004 by the American Association for the Advancement of Science

Zebrafish Dpr2 Inhibits Mesoderm Induction by Promoting Degradation of Nodal Receptors

Lixia Zhang,1* Hu Zhou,2* Ying Su,1* Zhihui Sun,1 Haiwen Zhang,1 Long Zhang,2 Yu Zhang,1 Yuanheng Ning,2 Ye-Guang Chen,2{dagger} Anming Meng1{dagger}

Abstract: Nodal proteins, members of the transforming growth factor–ß (TGFß) superfamily, have been identified as key endogenous mesoderm inducers in vertebrates. Precise control of Nodal signaling is essential for normal development of embryos. Here, we report that zebrafish dapper2 (dpr2) is expressed in mesoderm precursors during early embryogenesis and is positively regulated by Nodal signals. In vivo functional studies in zebrafish suggest that Dpr2 suppresses mesoderm induction activities of Nodal signaling. Dpr2 is localized in late endosomes, binds to the TGFß receptors ALK5 and ALK4, and accelerates lysosomal degradation of these receptors.

1 Laboratory of Developmental Biology,
2 Laboratory of Molecular Cell Biology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Protein Sciences Laboratory of the Ministry of Education (MOE), Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.

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* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: mengam{at} (A.M.) and ygchen{at} (Y.-G.C.)

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