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Science 306 (5693): 124-129

Copyright © 2004 by the American Association for the Advancement of Science

Src Mediates a Switch from Microtubule- to Actin-Based Motility of Vaccinia Virus

Timothy P. Newsome, Niki Scaplehorn, Michael Way*

Abstract: The cascade of events that leads to vaccinia-induced actin polymerization requires Src-dependent tyrosine phosphorylation of the viral membrane protein A36R. We found that a localized outside-in signaling cascade induced by the viral membrane protein B5R is required to potently activate Src and induce A36R phosphorylation at the plasma membrane. In addition, Src-mediated phosphorylation of A36R regulated the ability of virus particles to recruit and release conventional kinesin. Thus, Src activity regulates the transition between cytoplasmic microtubule transport and actin-based motility at the plasma membrane.

Cell Motility Laboratory, Room 529, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.

* To whom correspondence should be addressed. E-mail: michael.way{at}cancer.org.uk

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