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Science 306 (5701): 1508-1509

Copyright © 2004 by the American Association for the Advancement of Science

Pheromone Signaling Mechanisms in Yeast: A Prototypical Sex Machine

Yuqi Wang, and Henrik G. Dohlman*

Abstract: The actions of many extracellular stimuli are elicited by complexes of cell surface receptors, heterotrimeric guanine nucleotide–binding proteins (G proteins), and mitogen-activated protein (MAP) kinase complexes. Analysis of haploid yeast cells and their response to peptide mating pheromones has produced important advances in our understanding of G protein and MAP kinase signaling mechanisms. Many of the components, their interrelationships, and their regulators were first identified in yeast. Current analysis of the pheromone response pathway (see the Connections Maps at Science's Signal Transduction Knowledge Environment) will benefit from new and powerful genomic, proteomic, and computational approaches that will likely reveal additional general principles that are applicable to more complex organisms.

Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599–7260, USA.

* To whom correspondence should be addressed. E-mail: hdohlman{at}

Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling.
V. Yerko, T. Sulea, I. Ekiel, D. Harcus, J. Baardsnes, M. Cygler, M. Whiteway, and C. Wu (2013)
Mol. Biol. Cell 24, 409-420
   Abstract »    Full Text »    PDF »
Combined computational and experimental analysis reveals mitogen-activated protein kinase-mediated feedback phosphorylation as a mechanism for signaling specificity.
N. Hao, N. Yildirim, M. J. Nagiec, S. C. Parnell, B. Errede, H. G. Dohlman, and T. C. Elston (2012)
Mol. Biol. Cell 23, 3899-3910
   Abstract »    Full Text »    PDF »
Pheromone- and RSP5-dependent Ubiquitination of the G Protein {beta} Subunit Ste4 in Yeast.
M. Zhu, M. P. Torres, J. B. Kelley, H. G. Dohlman, and Y. Wang (2011)
J. Biol. Chem. 286, 27147-27155
   Abstract »    Full Text »    PDF »
The Putative Lipid Transporter, Arv1, Is Required for Activating Pheromone-Induced MAP Kinase Signaling in Saccharomyces cerevisiae.
M. L. Villasmil, A. Ansbach, and J. T. Nickels Jr. (2011)
Genetics 187, 455-465
   Abstract »    Full Text »    PDF »
Dynamic Localization of Fus3 Mitogen-Activated Protein Kinase Is Necessary To Evoke Appropriate Responses and Avoid Cytotoxic Effects.
R. E. Chen, J. C. Patterson, L. S. Goupil, and J. Thorner (2010)
Mol. Cell. Biol. 30, 4293-4307
   Abstract »    Full Text »    PDF »
Nucleus-Specific and Cell Cycle-Regulated Degradation of Mitogen-Activated Protein Kinase Scaffold Protein Ste5 Contributes to the Control of Signaling Competence.
L. S. Garrenton, A. Braunwarth, S. Irniger, E. Hurt, M. Kunzler, and J. Thorner (2009)
Mol. Cell. Biol. 29, 582-601
   Abstract »    Full Text »    PDF »
A Scaffold Makes the Switch.
H. G. Dohlman (2008)
Science Signaling 1, pe46
   Abstract »    Full Text »    PDF »
Down-regulation of Pkc1-mediated Signaling by the Deubiquitinating Enzyme Ubp3.
Y. Wang, M. Zhu, M. Ayalew, and J. A. Ruff (2008)
J. Biol. Chem. 283, 1954-1961
   Abstract »    Full Text »    PDF »
Detection of eQTL modules mediated by activity levels of transcription factors.
W. Sun, T. Yu, and K.-C. Li (2007)
Bioinformatics 23, 2290-2297
   Abstract »    Full Text »    PDF »
A role for a complex between activated G protein-coupled receptors in yeast cellular mating.
C. Shi, S. Kaminskyj, S. Caldwell, and M. C. Loewen (2007)
PNAS 104, 5395-5400
   Abstract »    Full Text »    PDF »
Regulation of G Protein and Mitogen-Activated Protein Kinase Signaling by Ubiquitination: Insights From Model Organisms.
Y. Wang and H. G. Dohlman (2006)
Circ. Res. 99, 1305-1314
   Abstract »    Full Text »    PDF »
Pheromone Signaling Pathways in Yeast.
H. G. Dohlman and J. E. Slessareva (2006)
Sci. STKE 2006, cm6
   Abstract »    Full Text »    PDF »
G Protein Signaling in Yeast: New Components, New Connections, New Compartments.
J. E. Slessareva and H. G. Dohlman (2006)
Science 314, 1412-1413
   Abstract »    Full Text »    PDF »
Analysis of Mitogen-Activated Protein Kinase Signaling Specificity in Response to Hyperosmotic Stress: Use of an Analog-Sensitive HOG1 Allele.
P. J. Westfall and J. Thorner (2006)
Eukaryot. Cell 5, 1215-1228
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Function of the MAPK scaffold protein, Ste5, requires a cryptic PH domain.
L. S. Garrenton, S. L. Young, and J. Thorner (2006)
Genes & Dev. 20, 1946-1958
   Abstract »    Full Text »    PDF »
The RA Domain of Ste50 Adaptor Protein Is Required for Delivery of Ste11 to the Plasma Membrane in the Filamentous Growth Signaling Pathway of the Yeast Saccharomyces cerevisiae.
D. M. Truckses, J. E. Bloomekatz, and J. Thorner (2006)
Mol. Cell. Biol. 26, 912-928
   Abstract »    Full Text »    PDF »
Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway.
A. Gruhler, J. V. Olsen, S. Mohammed, P. Mortensen, N. J. Faergeman, M. Mann, and O. N. Jensen (2005)
Mol. Cell. Proteomics 4, 310-327
   Abstract »    Full Text »    PDF »
Jekyll and Hyde in the Microbial World.
D. M. Truckses, L. S. Garrenton, and J. Thorner (2004)
Science 306, 1509-1511
   Abstract »    Full Text »    PDF »

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