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Science 306 (5703): 1947-1950

Copyright © 2004 by the American Association for the Advancement of Science

The Gs-Linked Receptor GPR3 Maintains Meiotic Arrest in Mammalian Oocytes

Lisa M. Mehlmann,1* Yoshinaga Saeki,2{dagger} Shigeru Tanaka,2{dagger} Thomas J. Brennan,3{ddagger} Alexei V. Evsikov,4 Frank L. Pendola,4 Barbara B. Knowles,4 John J. Eppig,4 Laurinda A. Jaffe1*

Abstract: Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype can be reversed by injection of Gpr3 RNA into the oocytes. Thus, the GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle and is crucial for the regulation of meiosis.

1 Department of Cell Biology, University of Connecticut Health Center (UCHC), Farmington, CT 06032, USA.
2 Massachusetts General Hospital, Charleston, MA 02129, USA.
3 Deltagen Inc., San Carlos, CA 94070, USA.
4 The Jackson Laboratory, Bar Harbor, ME 04609, USA.

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{dagger} Present address: Ohio State University, Columbus, OH 43210, USA.

{ddagger} Present address: Toccata Therapeutics, Menlo Park, CA, 94025, USA.

* To whom correspondence should be addressed. E-mail: lmehlmann{at}neuron.uchc.edu (L.M.M.); ljaffe{at}neuron.uchc.edu (L.A.J.)

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