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Science 306 (5705): 2255-2257

Copyright © 2004 by the American Association for the Advancement of Science

Mouse Brain Organization Revealed Through Direct Genome-Scale TF Expression Analysis

Paul A. Gray,1,3*{dagger} Hui Fu,2,3* Ping Luo,1,3* Qing Zhao,4 Jing Yu,5 Annette Ferrari,3 Toyoaki Tenzen,5 Dong-in Yuk,4 Eric F. Tsung,6{ddagger} Zhaohui Cai,6 John A. Alberta,3 Le-ping Cheng,1,3 Yang Liu,1,3 Jan M. Stenman,5 M. Todd Valerius,5 Nathan Billings,4 Haesun A. Kim,2,3§ Michael E. Greenberg,1,8 Andrew P. McMahon,5 David H. Rowitch,4,7 Charles D. Stiles,2,3|| Qiufu Ma1,3||

Abstract: In the developing brain, transcription factors (TFs) direct the formation of a diverse array of neurons and glia. We identifed 1445 putative TFs in the mouse genome. We used in situ hybridization to map the expression of over 1000 of these TFs and TF-coregulator genes in the brains of developing mice. We found that 349 of these genes showed restricted expression patterns that were adequate to describe the anatomical organization of the brain. We provide a comprehensive inventory of murine TFs and their expression patterns in a searchable brain atlas database.

1 Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
2 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
3 Department of Cancer Biology, Dana-Farber Cancer Institute, 1 Jimmy Fund Way, Boston, MA 02115, USA.
4 Department of Pediatric Oncology, Dana-Farber Cancer Institute, 1 Jimmy Fund Way, Boston, MA 02115, USA.
5 Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
6 Informatics Program, Children's Hospital, Boston, MA 02115, USA.
7 Division of Newborn Medicine, Children's Hospital, Boston, MA 02115, USA.
8 Division of Neuroscience, Children's Hospital, Boston, MA 02115, USA.

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* These authors contributed equally to this work.

{dagger} Present address: Molecular Neurobiology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

{ddagger} Present address: Department of Biology, Boston College, 140 Commonwealth Avenue, Chestnut Hill, MA 02467, USA.

§ Present address: Department of Biological Sciences, Rutgers University, 101 Warren Street, Newark, NJ 07102 USA.

|| To whom correspondence should be addressed. E-mail: Qiufu_Ma{at}dfci.harvard.edu (Q.M.); Charles_Stiles{at}dfci.harvard.edu (C.D.S.)

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