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Science 307 (5706): 124-127

Copyright © 2005 by the American Association for the Advancement of Science

Vesicle Endocytosis Requires Dynamin-Dependent GTP Hydrolysis at a Fast CNS Synapse

Takayuki Yamashita, Toshihide Hige, Tomoyuki Takahashi*

Abstract: Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as "kiss-and-run" was unrelated to transmitter release. The release-related capacitance change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine 5'-triphosphate (GTP) analog GTPgS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.

Department of Neurophysiology, University of Tokyo Graduate School of Medicine, Tokyo 113-0033, Japan.

* To whom correspondence should be addressed. E-mail: ttakahas-tky{at}umin.ac.jp

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