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Science 307 (5706): 130-133

Copyright © 2005 by the American Association for the Advancement of Science

The Centromeric Protein Sgo1 Is Required to Sense Lack of Tension on Mitotic Chromosomes

Vahan B. Indjeian, Bodo M. Stern,* Andrew W. Murray{dagger}

Abstract: Chromosome alignment on the mitotic spindle is monitored by the spindle checkpoint. We identify Sgo1, a protein involved in meiotic chromosome cohesion, as a spindle checkpoint component. Budding yeast cells with mutations in SGO1 respond normally to microtubule depolymerization but not to lack of tension at the kinetochore, and they have difficulty attaching sister chromatids to opposite poles of the spindle. Sgo1 is thus required for sensing tension between sister chromatids during mitosis, and its degradation when they separate may prevent cell cycle arrest and chromosome loss in anaphase, a time when sister chromatids are no longer under tension.

Department of Molecular and Cellular Biology, Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.

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* Present address: Cell Press, 1100 Massachusetts Avenue, Cambridge, MA 02138, USA.

{dagger} To whom correspondence should be addressed. E-mail: amurray{at}mcb.harvard.edu

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