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Science 307 (5709): 600-604

Copyright © 2005 by the American Association for the Advancement of Science

Illumination of the Melanopsin Signaling Pathway

Satchidananda Panda,*{dagger}{ddagger} Surendra K. Nayak,* Brice Campo, John R. Walker, John B. Hogenesch,§ Tim Jegla{dagger}

Abstract: In mammals, a small population of intrinsically photosensitive retinal ganglion cells (ipRGCs) plays a key role in the regulation of nonvisual photic responses, such as behavioral responses to light, pineal melatonin synthesis, pupillary light reflex, and sleep latency. These ipRGCs also express melanopsin (Opn4), a putative opsin-family photopigment that has been shown to play a role in mediating these nonvisual photic responses. Melanopsin is required for the function of this inner retinal pathway, but its precise role in generating photic responses has not yet been determined. We found that expression of melanopsin in Xenopus oocytes results in light-dependent activation of membrane currents through the G{alpha}q/G{alpha}11 G protein pathway, with an action spectrum closely matching that of melanopsin-expressing ipRGCs and of behavioral responses to light in mice lacking rods and cones. When coexpressed with arrestins, melanopsin could use all-trans-retinaldehyde as a chromophore, which suggests that it may function as a bireactive opsin. We also found that melanopsin could activate the cation channel TRPC3, a mammalian homolog of the Drosophila phototransduction channels TRP and TRPL. Melanopsin therefore signals more like an invertebrate opsin than like a classical vertebrate rod-and-cone opsin.

Genomics Institute of Novartis Research Foundation, 10675 John J. Hopkins Drive, San Diego, CA 92121, USA.

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Note added in proof: Similar findings have recently been reported (30, 31).

* These authors contributed equally to this work.

{ddagger} Present address: Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

§ Present address: Scripps Florida, FAU-Building MC-17, 5353 Parkside Drive, Jupiter, FL 33458, USA.

{dagger} To whom correspondence should be addressed. E-mail: satchin{at}, tjegla{at}

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