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Science 308 (5719): 252-255

Copyright © 2005 by the American Association for the Advancement of Science

Antigen Recognition Determinants of {gamma}{delta} T Cell Receptors

Sunny Shin,1 Ramy El-Diwany,1,2 Steven Schaffert,1 Erin J. Adams,1,2 K. Christopher Garcia,1,2 Pablo Pereira,3 Yueh-hsiu Chien1,2*

Abstract: The molecular basis of {gamma}{delta} T cell receptor (TCR) recognition is poorly understood. Here, we analyze the TCR sequences of a natural {gamma}{delta} T cell population specific for the major histocompatibility complex class Ib molecule T22. We find that T22 recognition correlates strongly with a somatically recombined TCR{delta} complementarity-determining region 3 (CDR3) motif derived from germ line–encoded residues. Sequence diversity around these residues modulates TCR ligand-binding affinities, whereas V gene usage correlates mainly with tissue origin. These results show how an antigen-specific {gamma}{delta} TCR repertoire can be generated at a high frequency and suggest that {gamma}{delta} T cells recognize a limited number of antigens.

1 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
2 Department of Program in Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
3 Unité du Développement des Lymphocytes, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1961, Institut Pasteur, 75724 Paris, France.

* To whom correspondence should be addressed. E-mail: chien{at}stanford.edu

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