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Science 309 (5731): 140-142

Copyright © 2005 by the American Association for the Advancement of Science

tRNA Actively Shuttles Between the Nucleus and Cytosol in Yeast

Akira Takano,1 Toshiya Endo,1,3,4 Tohru Yoshihisa1,2*

Abstract: Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription. Import requires energy but not the Ran cycle. These results indicate that tRNAs shuttle between the nucleus and cytosol.

1 Department of Chemistry, Graduate School of Science, Japan Science and Technology Corporation, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
2 Research Center for Materials Science, Japan Science and Technology Corporation, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
3 Institute for Advanced Research, Japan Science and Technology Corporation, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
4 Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.

* To whom all correspondence should be addressed. E-mail: tyoshihi{at}

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