Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 309 (5735): 774-777

Copyright © 2005 by the American Association for the Advancement of Science

Recognition of Host Immune Activation by Pseudomonas aeruginosa

Licheng Wu,1 Oscar Estrada,1 Olga Zaborina,1 Manjeet Bains,4 Le Shen,2 Jonathan E. Kohler,1 Nachiket Patel,1 Mark W. Musch,3 Eugene B. Chang,3 Yang-Xin Fu,2 Michael A. Jacobs,5 Michael I. Nishimura,1 Robert E. W. Hancock,4 Jerrold R. Turner,2 John C. Alverdy1*

Abstract: It is generally reasoned that lethal infections caused by opportunistic pathogens develop permissively by invading a host that is both physiologically stressed and immunologically compromised. However, an alternative hypothesis might be that opportunistic pathogens actively sense alterations in host immune function and respond by enhancing their virulence phenotype. We demonstrate that interferon-{gamma} binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin. These observations provide details of the mechanisms by which prokaryotic organisms are directly signaled by immune activation in their eukaryotic host.

1 Department of Surgery, University of Chicago, Pritzker School of Medicine, Chicago, IL 60637, USA.
2 Department of Pathology, University of Chicago, Pritzker School of Medicine, Chicago, IL 60637, USA.
3 Department of Medicine, University of Chicago, Pritzker School of Medicine, Chicago, IL 60637, USA.
4 Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T1Z4, Canada.
5 Department of Genome Sciences and Medicine, University of Washington, Seattle, WA 98195, USA.

* To whom correspondence should be addressed. E-mail: jalverdy{at}surgery.bsd.uchicago.edu


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pro-inflammatory cytokines can act as intracellular modulators of commensal bacterial virulence.
J. Mahdavi, P.-J. Royer, H. S. Sjolinder, S. Azimi, T. Self, J. Stoof, L. M. Wheldon, K. Brannstrom, R. Wilson, J. Moreton, et al. (2013)
Open Bio 3, 130048
   Abstract »    Full Text »    PDF »
Transcription of the oprF Gene of Pseudomonas aeruginosa Is Dependent Mainly on the SigX Sigma Factor and Is Sucrose Induced.
E. Bouffartigues, G. Gicquel, A. Bazire, M. Bains, O. Maillot, J. Vieillard, M. G. J. Feuilloley, N. Orange, R. E. W. Hancock, A. Dufour, et al. (2012)
J. Bacteriol. 194, 4301-4311
   Abstract »    Full Text »    PDF »
C-type natriuretic peptide modulates quorum sensing molecule and toxin production in Pseudomonas aeruginosa.
A.-S. Blier, W. Veron, A. Bazire, E. Gerault, L. Taupin, J. Vieillard, K. Rehel, A. Dufour, F. Le Derf, N. Orange, et al. (2011)
Microbiology 157, 1929-1944
   Abstract »    Full Text »    PDF »
Full Virulence of Pseudomonas aeruginosa Requires OprF.
L. Fito-Boncompte, A. Chapalain, E. Bouffartigues, H. Chaker, O. Lesouhaitier, G. Gicquel, A. Bazire, A. Madi, N. Connil, W. Veron, et al. (2011)
Infect. Immun. 79, 1176-1186
   Abstract »    Full Text »    PDF »
Host Chemokines Bind to Staphylococcus aureus and Stimulate Protein A Release.
S. C. Yung, D. Parenti, and P. M. Murphy (2011)
J. Biol. Chem. 286, 5069-5077
   Abstract »    Full Text »    PDF »
The outer membrane protein OprQ and adherence of Pseudomonas aeruginosa to human fibronectin.
A. Arhin and C. Boucher (2010)
Microbiology 156, 1415-1423
   Abstract »    Full Text »    PDF »
Oral PEG 15-20 protects the intestine against radiation: role of lipid rafts.
V. Valuckaite, O. Zaborina, J. Long, M. Hauer-Jensen, J. Wang, C. Holbrook, A. Zaborin, K. Drabik, M. Katdare, H. Mauceri, et al. (2009)
Am J Physiol Gastrointest Liver Physiol 297, G1041-G1052
   Abstract »    Full Text »    PDF »
Shielding, a new pathogen defence mechanism against PMNs.
P. Cornelis (2009)
Microbiology 155, 3474-3475
   Full Text »    PDF »
Flagellum-Mediated Biofilm Defense Mechanisms of Pseudomonas aeruginosa against Host-Derived Lactoferrin.
J. G. Leid, M. Kerr, C. Selgado, C. Johnson, G. Moreno, A. Smith, M. E. Shirtliff, G. A. O'Toole, and E. K. Cope (2009)
Infect. Immun. 77, 4559-4566
   Abstract »    Full Text »    PDF »
Proteomic Identification of OprL as a Seromarker for Initial Diagnosis of Pseudomonas aeruginosa Infection of Patients with Cystic Fibrosis.
A. R. Rao, A. Laxova, P. M. Farrell, and J. T. Barbieri (2009)
J. Clin. Microbiol. 47, 2483-2488
   Abstract »    Full Text »    PDF »
Genomewide Identification of Genetic Determinants of Antimicrobial Drug Resistance in Pseudomonas aeruginosa.
A. Dotsch, T. Becker, C. Pommerenke, Z. Magnowska, L. Jansch, and S. Haussler (2009)
Antimicrob. Agents Chemother. 53, 2522-2531
   Abstract »    Full Text »    PDF »
Red death in Caenorhabditis elegans caused by Pseudomonas aeruginosa PAO1.
A. Zaborin, K. Romanowski, S. Gerdes, C. Holbrook, F. Lepine, J. Long, V. Poroyko, S. P. Diggle, A. Wilke, K. Righetti, et al. (2009)
PNAS 106, 6327-6332
   Abstract »    Full Text »    PDF »
Neutrophil Elastase Mediates Innate Host Protection against Pseudomonas aeruginosa.
T. O. Hirche, R. Benabid, G. Deslee, S. Gangloff, S. Achilefu, M. Guenounou, F. Lebargy, R. E. Hancock, and A. Belaaouaj (2008)
J. Immunol. 181, 4945-4954
   Abstract »    Full Text »    PDF »
Role of quorum sensing by Pseudomonas aeruginosa in microbial keratitis and cystic fibrosis.
M. D. P. Willcox, H. Zhu, T. C. R. Conibear, E. B. H. Hume, M. Givskov, S. Kjelleberg, and S. A. Rice (2008)
Microbiology 154, 2184-2194
   Abstract »    Full Text »    PDF »
The re-emerging role of the intestinal microflora in critical illness and inflammation: why the gut hypothesis of sepsis syndrome will not go away.
J. C. Alverdy and E. B. Chang (2008)
J. Leukoc. Biol. 83, 461-466
   Abstract »    Full Text »    PDF »
Quorum sensing, communication and cross-kingdom signalling in the bacterial world.
P. Williams (2007)
Microbiology 153, 3923-3938
   Abstract »    Full Text »    PDF »
Look who's talking: communication and quorum sensing in the bacterial world.
P. Williams, K. Winzer, W. C Chan, and M. Camara (2007)
Phil Trans R Soc B 362, 1119-1134
   Abstract »    Full Text »    PDF »
Environmental Regulation of Pseudomonas aeruginosa PAO1 Las and Rhl Quorum-Sensing Systems.
K. Duan and M. G. Surette (2007)
J. Bacteriol. 189, 4827-4836
   Abstract »    Full Text »    PDF »
Recognition of intestinal epithelial HIF-1{alpha} activation by Pseudomonas aeruginosa.
N. J. Patel, O. Zaborina, L. Wu, Y. Wang, D. J. Wolfgeher, V. Valuckaite, M. J. Ciancio, J. E. Kohler, O. Shevchenko, S. P. Colgan, et al. (2007)
Am J Physiol Gastrointest Liver Physiol 292, G134-G142
   Abstract »    Full Text »    PDF »
Neutrophil Elastase, an Innate Immunity Effector Molecule, Represses Flagellin Transcription in Pseudomonas aeruginosa.
A. Sonawane, J. Jyot, R. During, and R. Ramphal (2006)
Infect. Immun. 74, 6682-6689
   Abstract »    Full Text »    PDF »
Molecular evolution of the major outer-membrane protein gene (oprF) of Pseudomonas..
J. Bodilis and S. Barray (2006)
Microbiology 152, 1075-1088
   Abstract »    Full Text »    PDF »
Pathogens fight back.
D. Secko (2005)
Can. Med. Assoc. J. 173, 864
   Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882