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Science 310 (5745): 121-125

Copyright © 2005 by the American Association for the Advancement of Science

Arabidopsis H+-PPase AVP1 Regulates Auxin-Mediated Organ Development

Jisheng Li,1* Haibing Yang,1* Wendy Ann Peer,3 Gregory Richter,2 Joshua Blakeslee,3 Anindita Bandyopadhyay,3 Boosaree Titapiwantakun,3 Soledad Undurraga,1 Mariya Khodakovskaya,1 Elizabeth L. Richards,3 Beth Krizek,4 Angus S. Murphy,3 Simon Gilroy,2 Roberto Gaxiola1{dagger}

Abstract: The transport of auxin controls developmental events in plants. Here, we report that in addition to maintaining vacuolar pH, the H+-pyrophosphatase, AVP1, controls auxin transport and consequently auxin-dependent development. AVP1 overexpression results in increased cell division at the onset of organ formation, hyperplasia, and increased auxin transport. In contrast, avp1-1 null mutants have severely disrupted root and shoot development and reduced auxin transport. Changes in the expression of AVP1 affect the distribution and abundance of the P–adenosine triphosphatase and Pinformed 1 auxin efflux facilitator, two proteins implicated in auxin distribution. Thus, AVP1 facilitates the auxin fluxes that regulate organogenesis.

1 Department of Plant Science, University of Connecticut, Storrs, CT 06268, USA.
2 Biology Department, Pennsylvania State University, University Park, PA 16802, USA.
3 Horticulture Department, Purdue University, West Lafayette, IN 47907, USA.
4 Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: roberto.gaxiola{at}uconn.edu

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