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Science 310 (5753): 1492-1495

Copyright © 2005 by the American Association for the Advancement of Science

A Role for the Phagosome in Cytokine Secretion

Rachael Z. Murray, Jason G. Kay, Daniele G. Sangermani, Jennifer L. Stow*

Abstract: Membrane traffic in activated macrophages is required for two critical events in innate immunity: proinflammatory cytokine secretion and phagocytosis of pathogens. We found a joint trafficking pathway linking both actions, which may economize membrane transport and augment the immune response. Tumor necrosis factor {alpha} (TNF{alpha}) is trafficked from the Golgi to the recycling endosome (RE), where vesicle-associated membrane protein 3 mediates its delivery to the cell surface at the site of phagocytic cup formation. Fusion of the RE at the cup simultaneously allows rapid release of TNF{alpha} and expands the membrane for phagocytosis.

Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia.

* To whom correspondence should be addressed. E-mail: j.stow{at}

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