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Science 310 (5756): 1957-1960

Copyright © 2005 by the American Association for the Advancement of Science

fgf20 Is Essential for Initiating Zebrafish Fin Regeneration

Geoffrey G. Whitehead, Shinji Makino, Ching-Ling Lien, Mark T. Keating*

Abstract: Epimorphic regeneration requires the presence or creation of pluripotent cells capable of reproducing lost organs. Zebrafish fin regeneration is mediated by the creation of blastema cells. Here, we characterize the devoid of blastema (dob) mutant that fails fin regeneration during initial steps, forms abnormal regeneration epithelium, and does not form blastema. This mutation has no impact on embryonic survival. Dob results from an fgf20a null mutation, Y148S. Fgf20a is expressed during initiation of fin regeneration at the epithelial-mesenchymal boundary and later overlaps with the blastema marker msxb. Thus, fgf20a has a regeneration-specific requirement, initiating fin regeneration, and controlling blastema formation.

Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Department of Cardiology, Children's Hospital, Boston, MA 02115, USA.

* To whom correspondence should be addressed. E-mail: mark.keating{at}novartis.com

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