Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Probing Cellular Chemistry in Biological Systems with Microelectrodes
R. Mark Wightman
Over the past 20 years, the technological impediments to fabricatingelectrodes of micrometer dimensions have been largely overcome.These small electrodes can be readily applied to probe chemicalevents at the surface of tissues or individual biological cells;they can even be used to monitor concentration changes withinintact animals. These measurements can be made on rapid timescales and with minimal perturbation of the system under study.Several recent applications have provided important insightsinto chemical processes at cells and in tissues. Examples includemolecular flux measurements at the surface of single cells andthrough skinwhich can offer insights into oxidative stress,exocytosis, and drug deliveryand real-time brain neurotransmittermonitoring in living rats, which reveals correlations betweenbehavior and molecular events in the brain. Such findings canpromote interdisciplinary collaborations and may lead to a broaderunderstanding of the chemical aspects of biology.
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
The editors suggest the following Related Resources on Science sites:
In Science Magazine
INTRODUCTION TO SPECIAL ISSUE
Nancy Gough, Phil Szuromi, and Jake Yeston (17 March 2006) Science311 (5767), 1565.
[DOI: 10.1126/science.311.5767.1565] |Summary »|PDF »
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Subsecond Dopamine Release in the Nucleus Accumbens Predicts Conditioned Punishment and Its Successful Avoidance.