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Science 312 (5779): 1520-1523

Copyright © 2006 by the American Association for the Advancement of Science

TOPLESS Regulates Apical Embryonic Fate in Arabidopsis

Jeff A. Long,1* Carolyn Ohno,2 Zachery R. Smith,1 Elliot M. Meyerowitz2

Abstract: The embryos of seed plants develop with an apical shoot pole and a basal root pole. In Arabidopsis, the topless-1 (tpl-1) mutation transforms the shoot pole into a second root pole. Here, we show that TPL resembles known transcriptional corepressors and that tpl-1 acts as a dominant negative mutation for multiple TPL-related proteins. Mutations in the putative coactivator HISTONE ACETYLTRANSFERASE GNAT SUPERFAMILY1 suppress the tpl-1 phenotype. Mutations in HISTONE DEACETYLASE19, a putative corepressor, increase the penetrance of tpl-1 and display similar apical defects. These data point to a transcriptional repression mechanism that prevents root formation in the shoot pole during Arabidopsis embryogenesis.

1 Plant Biology Laboratory, The Salk Institute for Biological Sciences, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
2 Division of Biology, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.

* To whom correspondence should be addressed. E-mail: long{at}salk.edu


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