Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

Science 313 (5783): 108-111

Copyright © 2006 by the American Association for the Advancement of Science

Polo-Like Kinase Cdc5 Controls the Local Activation of Rho1 to Promote Cytokinesis

Satoshi Yoshida,1 Keiko Kono,1,2 Drew M. Lowery,3 Sara Bartolini,1 Michael B. Yaffe,3 Yoshikazu Ohya,2 David Pellman1*

Abstract: The links between the cell cycle machinery and the cytoskeletal proteins controlling cytokinesis are poorly understood. The small guanine nucleotide triphosphate (GTP)–binding protein RhoA stimulates type II myosin contractility and formin-dependent assembly of the cytokinetic actin contractile ring. We found that budding yeast Polo-like kinase Cdc5 controls the targeting and activation of Rho1 (RhoA) at the division site via Rho1 guanine nucleotide exchange factors. This role of Cdc5 (Polo-like kinase) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms.

1 Department of Pediatric Oncology, Dana-Farber Cancer Institute and Division of Hematology/Oncology, Children's Hospital Boston and Harvard Medical School, Boston, MA 02115, USA.
2 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan.
3 Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

* To whom correspondence should be addressed. E-mail: david_pellman{at}

Global Analysis of Cdc14 Dephosphorylation Sites Reveals Essential Regulatory Role in Mitosis and Cytokinesis.
L. Kao, Y.-T. Wang, Y.-C. Chen, S.-F. Tseng, J.-C. Jhang, Y.-J. Chen, and S.-C. Teng (2014)
Mol. Cell. Proteomics 13, 594-605
   Abstract »    Full Text »    PDF »
Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis.
Y.-H. Zhu, Y. Ye, Z. Wu, and J.-Q. Wu (2013)
Mol. Biol. Cell 24, 3187-3204
   Abstract »    Full Text »    PDF »
Inhibition of Cdc42 during mitotic exit is required for cytokinesis.
B. D. Atkins, S. Yoshida, K. Saito, C.-F. Wu, D. J. Lew, and D. Pellman (2013)
J. Cell Biol. 202, 231-240
   Abstract »    Full Text »    PDF »
Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis.
M. Onishi, N. Ko, R. Nishihama, and J. R. Pringle (2013)
J. Cell Biol. 202, 311-329
   Abstract »    Full Text »    PDF »
Timing it right: Precise ON/OFF switches for Rho1 and Cdc42 GTPases in cytokinesis.
M. K. Balasubramanian and E. Y. Tao (2013)
J. Cell Biol. 202, 187-189
   Abstract »    Full Text »    PDF »
Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast.
C. Wloka, E. A. Vallen, L. The, X. Fang, Y. Oh, and E. Bi (2013)
J. Cell Biol. 200, 271-286
   Abstract »    Full Text »    PDF »
Mitotic Exit and Separation of Mother and Daughter Cells.
E. L. Weiss (2012)
Genetics 192, 1165-1202
   Abstract »    Full Text »    PDF »
Regulation of the formin Bnr1 by septins anda MARK/Par1-family septin-associated kinase.
S. M. Buttery, K. Kono, E. Stokasimov, and D. Pellman (2012)
Mol. Biol. Cell 23, 4041-4053
   Abstract »    Full Text »    PDF »
{alpha}-Synuclein disrupts stress signaling by inhibiting polo-like kinase Cdc5/Plk2.
S. Wang, B. Xu, L.-C. Liou, Q. Ren, S. Huang, Y. Luo, Z. Zhang, and S. N. Witt (2012)
PNAS 109, 16119-16124
   Abstract »    Full Text »    PDF »
The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth.
A. Sanchez-Diaz, P. J. Nkosi, S. Murray, and K. Labib (2012)
EMBO J. 31, 3620-3634
   Abstract »    Full Text »    PDF »
Cell Polarization and Cytokinesis in Budding Yeast.
E. Bi and H.-O. Park (2012)
Genetics 191, 347-387
   Abstract »    Full Text »    PDF »
Functional specialisation of yeast Rho1 GTP exchange factors.
S. A. Krause, M. J. Cundell, P. P. Poon, J. McGhie, G. C. Johnston, C. Price, and J. V. Gray (2012)
J. Cell Sci. 125, 2721-2731
   Abstract »    Full Text »    PDF »
Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis.
Y. Ye, I.-J. Lee, K. W. Runge, and J.-Q. Wu (2012)
Mol. Biol. Cell 23, 1181-1195
   Abstract »    Full Text »    PDF »
Morphogenesis and the Cell Cycle.
A. S. Howell and D. J. Lew (2012)
Genetics 190, 51-77
   Abstract »    Full Text »    PDF »
Regulation of Cell Wall Biogenesis in Saccharomyces cerevisiae: The Cell Wall Integrity Signaling Pathway.
D. E. Levin (2011)
Genetics 189, 1145-1175
   Abstract »    Full Text »    PDF »
Independent modulation of the kinase and polo-box activities of Cdc5 protein unravels unique roles in the maintenance of genome stability.
H. Ratsima, A.-M. Ladouceur, M. Pascariu, V. Sauve, Z. Salloum, P. S. Maddox, and D. D'Amours (2011)
PNAS 108, E914-E923
   Abstract »    Full Text »    PDF »
The Rho1 GTPase Acts Together With a Vacuolar Glutathione S-Conjugate Transporter to Protect Yeast Cells From Oxidative Stress.
M. E. Lee, K. Singh, J. Snider, A. Shenoy, C. M. Paumi, I. Stagljar, and H.-O. Park (2011)
Genetics 188, 859-870
   Abstract »    Full Text »    PDF »
Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis.
F. Meitinger, M. E. Boehm, A. Hofmann, B. Hub, H. Zentgraf, W. D. Lehmann, and G. Pereira (2011)
Genes & Dev. 25, 875-888
   Abstract »    Full Text »    PDF »
Global Analysis of Cdc14 Phosphatase Reveals Diverse Roles in Mitotic Processes.
J. Bloom, I. M. Cristea, A. L. Procko, V. Lubkov, B. T. Chait, M. Snyder, and F. R. Cross (2011)
J. Biol. Chem. 286, 5434-5445
   Abstract »    Full Text »    PDF »
Dbf4 Regulates the Cdc5 Polo-like Kinase through a Distinct Non-canonical Binding Interaction.
Y.-C. Chen and M. Weinreich (2010)
J. Biol. Chem. 285, 41244-41254
   Abstract »    Full Text »    PDF »
Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast.
F. Meitinger, B. Petrova, I. M. Lombardi, D. T. Bertazzi, B. Hub, H. Zentgraf, and G. Pereira (2010)
J. Cell Sci. 123, 1851-1861
   Abstract »    Full Text »    PDF »
ABC Transporters in Saccharomyces cerevisiae and Their Interactors: New Technology Advances the Biology of the ABCC (MRP) Subfamily.
C. M. Paumi, M. Chuk, J. Snider, I. Stagljar, and S. Michaelis (2009)
Microbiol. Mol. Biol. Rev. 73, 577-593
   Abstract »    Full Text »    PDF »
Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae.
R. Nishihama, J. H. Schreiter, M. Onishi, E. A. Vallen, J. Hanna, K. Moravcevic, M. F. Lippincott, H. Han, M. A. Lemmon, J. R. Pringle, et al. (2009)
J. Cell Biol. 185, 995-1012
   Abstract »    Full Text »    PDF »
Single cells (put a ring on it).
B. A. Wolfe and M. Glotzer (2009)
Genes & Dev. 23, 896-901
   Abstract »    Full Text »    PDF »
Mechanisms for concentrating Rho1 during cytokinesis.
S. Yoshida, S. Bartolini, and D. Pellman (2009)
Genes & Dev. 23, 810-823
   Abstract »    Full Text »    PDF »
Predicting Protein Post-translational Modifications Using Meta-analysis of Proteome Scale Data Sets.
D. Schwartz, M. F. Chou, and G. M. Church (2009)
Mol. Cell. Proteomics 8, 365-379
   Abstract »    Full Text »    PDF »
The Anaphase-promoting Complex Promotes Actomyosin-Ring Disassembly during Cytokinesis in Yeast.
G. H. Tully, R. Nishihama, J. R. Pringle, and D. O. Morgan (2009)
Mol. Biol. Cell 20, 1201-1212
   Abstract »    Full Text »    PDF »
Stepping into the ring: the SIN takes on contractile ring assembly.
R. H. Roberts-Galbraith and K. L. Gould (2008)
Genes & Dev. 22, 3082-3088
   Abstract »    Full Text »    PDF »
The Synthetic Genetic Network around PKC1 Identifies Novel Modulators and Components of Protein Kinase C Signaling in Saccharomyces cerevisiae.
S. A. Krause, H. Xu, and J. V. Gray (2008)
Eukaryot. Cell 7, 1880-1887
   Abstract »    Full Text »    PDF »
Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in Maintaining TOR Complex 2 Integrity and Coupling to Downstream Signaling.
H.-L. Ho, H.-Y. Lee, H.-C. Liao, and M.-Y. Chen (2008)
Eukaryot. Cell 7, 1328-1343
   Abstract »    Full Text »    PDF »
G1/S Cyclin-dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.
K. Kono, S. Nogami, M. Abe, M. Nishizawa, S. Morishita, D. Pellman, and Y. Ohya (2008)
Mol. Biol. Cell 19, 1763-1771
   Abstract »    Full Text »    PDF »
Polo-like Kinase 1 Is Involved in Invasion through Extracellular Matrix.
A. Rizki, J. D. Mott, and M. J. Bissell (2007)
Cancer Res. 67, 11106-11110
   Abstract »    Full Text »    PDF »
Activation of the Cdc42p GTPase by cyclin-dependent protein kinases in budding yeast.
R. Sopko, D. Huang, J. C. Smith, D. Figeys, and B. J. Andrews (2007)
EMBO J. 26, 4487-4500
   Abstract »    Full Text »    PDF »
Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate.
D. M. Lowery, K. R. Clauser, M. Hjerrild, D. Lim, J. Alexander, K. Kishi, S.-E. Ong, S. Gammeltoft, S. A. Carr, and M. B. Yaffe (2007)
EMBO J. 26, 2262-2273
   Abstract »    Full Text »    PDF »
Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables.
S. M. Buttery, S. Yoshida, and D. Pellman (2007)
Mol. Biol. Cell 18, 1826-1838
   Abstract »    Full Text »    PDF »
Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells.
M. E. Burkard, C. L. Randall, S. Larochelle, C. Zhang, K. M. Shokat, R. P. Fisher, and P. V. Jallepalli (2007)
PNAS 104, 4383-4388
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882