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Science 313 (5783): 68-71

Copyright © 2006 by the American Association for the Advancement of Science

Hierarchical Action and Inhibition of Plant Dicer-Like Proteins in Antiviral Defense

Angélique Deleris,1 Javier Gallego-Bartolome,1 Jinsong Bao,2 Kristin D. Kasschau,2 James C. Carrington,2 Olivier Voinnet1*

Abstract: The mechanisms underlying induction and suppression of RNA silencing in the ongoing plant-virus arms race are poorly understood. We show here that virus-derived small RNAs produced by Arabidopsis Dicer-like 4 (DCL4) program an effector complex conferring antiviral immunity. Inhibition of DCL4 by a viral-encoded suppressor revealed the subordinate antiviral activity of DCL2. Accordingly, inactivating both DCL2 and DCL4 was necessary and sufficient to restore systemic infection of a suppressor-deficient virus. The effects of DCL2 were overcome by increasing viral dosage in inoculated leaves, but this could not surmount additional, non–cell autonomous effects of DCL4 specifically preventing viral unloading from the vasculature. These findings define a molecular framework for studying antiviral silencing and defense in plants.

1 Institut de Biologie Moléculaire des Plantes, CNRS Unité Propre de Recherche (UPR) 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France.
2 Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331, USA.

* To whom correspondence should be addressed. E-mail: olivier.voinnet{at}

Novel functional small RNAs are selectively loaded onto mammalian Ago1.
N. Yamakawa, K. Okuyama, J. Ogata, A. Kanai, A. Helwak, M. Takamatsu, K.-i. Imadome, K. Takakura, B. Chanda, N. Kurosaki, et al. (2014)
Nucleic Acids Res.
   Abstract »    Full Text »    PDF »
Arabidopsis Double-Stranded RNA Binding Protein DRB3 Participates in Methylation-Mediated Defense against Geminiviruses.
P. Raja, J. N. Jackel, S. Li, I. M. Heard, and D. M. Bisaro (2014)
J. Virol. 88, 2611-2622
   Abstract »    Full Text »    PDF »
Using a Viral Vector to Reveal the Role of MicroRNA159 in Disease Symptom Induction by a Severe Strain of Cucumber mosaic virus.
Z. Du, A. Chen, W. Chen, J. H. Westwood, D. C. Baulcombe, and J. P. Carr (2014)
Plant Physiology 164, 1378-1388
   Abstract »    Full Text »    PDF »
Distinct substrate specificities of Arabidopsis DCL3 and DCL4.
H. Nagano, A. Fukudome, A. Hiraguri, H. Moriyama, and T. Fukuhara (2014)
Nucleic Acids Res. 42, 1845-1856
   Abstract »    Full Text »    PDF »
Binding and processing of small dsRNA molecules by the class 1 RNase III protein encoded by sweet potato chlorotic stunt virus.
I. Weinheimer, K. Boonrod, M. Moser, M. Wassenegger, G. Krczal, S. J. Butcher, and J. P. T. Valkonen (2014)
J. Gen. Virol. 95, 486-495
   Abstract »    Full Text »    PDF »
Global and local perturbation of the tomato microRNA pathway by a trans-activated DICER-LIKE 1 mutant.
M. Kravchik, R. Sunkar, S. Damodharan, R. Stav, M. Zohar, T. Isaacson, and T. Arazi (2014)
J. Exp. Bot. 65, 725-739
   Abstract »    Full Text »    PDF »
RNA Interference Functions as an Antiviral Immunity Mechanism in Mammals.
Y. Li, J. Lu, Y. Han, X. Fan, and S.-W. Ding (2013)
Science 342, 231-234
   Abstract »    Full Text »    PDF »
Antiviral RNA Interference in Mammalian Cells.
P. V. Maillard, C. Ciaudo, A. Marchais, Y. Li, F. Jay, S. W. Ding, and O. Voinnet (2013)
Science 342, 235-238
   Abstract »    Full Text »    PDF »
Homologous RIG-I-like helicase proteins direct RNAi-mediated antiviral immunity in C. elegans by distinct mechanisms.
X. Guo, R. Zhang, J. Wang, S.-W. Ding, and R. Lu (2013)
PNAS 110, 16085-16090
   Abstract »    Full Text »    PDF »
Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs.
K. Rogers and X. Chen (2013)
PLANT CELL 25, 2383-2399
   Abstract »    Full Text »    PDF »
WRKY8 transcription factor functions in the TMV-cg defense response by mediating both abscisic acid and ethylene signaling in Arabidopsis.
L. Chen, L. Zhang, D. Li, F. Wang, and D. Yu (2013)
PNAS 110, E1963-E1971
   Abstract »    Full Text »    PDF »
AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system.
J. Schuck, T. Gursinsky, V. Pantaleo, J. Burgyan, and S.-E. Behrens (2013)
Nucleic Acids Res. 41, 5090-5103
   Abstract »    Full Text »    PDF »
Evolution of Animal and Plant Dicers: Early Parallel Duplications and Recurrent Adaptation of Antiviral RNA Binding in Plants.
K. Mukherjee, H. Campos, and B. Kolaczkowski (2013)
Mol. Biol. Evol. 30, 627-641
   Abstract »    Full Text »    PDF »
Effects of Defective Interfering RNA on Symptom Induction by, and Replication of, a Novel Partitivirus from a Phytopathogenic Fungus, Rosellinia necatrix.
S. Chiba, Y.-H. Lin, H. Kondo, S. Kanematsu, and N. Suzuki (2013)
J. Virol. 87, 2330-2341
   Abstract »    Full Text »    PDF »
A deletion polymorphism in the Caenorhabditis elegans RIG-I homolog disables viral RNA dicing and antiviral immunity.
A. Ashe, T. Belicard, J. Le Pen, P. Sarkies, L. Frezal, N. J. Lehrbach, M.-A. Felix, and E. A. Miska (2013)
eLife Sci 2, e00994
   Abstract »    Full Text »    PDF »
DICER-LIKE3 Activity in Physcomitrella patens DICER-LIKE4 Mutants Causes Severe Developmental Dysfunction and Sterility.
M. A. Arif, I. Fattash, Z. Ma, S. H. Cho, A. K. Beike, R. Reski, M. J. Axtell, and W. Frank (2012)
Mol Plant 5, 1281-1294
   Abstract »    Full Text »    PDF »
Roles of DICER-LIKE and ARGONAUTE Proteins in TAS-Derived Small Interfering RNA-Triggered DNA Methylation.
L. Wu, L. Mao, and Y. Qi (2012)
Plant Physiology 160, 990-999
   Abstract »    Full Text »    PDF »
Mutations in the Arabidopsis H3K4me2/3 Demethylase JMJ14 Suppress Posttranscriptional Gene Silencing by Decreasing Transgene Transcription.
I. Le Masson, V. Jauvion, N. Bouteiller, M. Rivard, T. Elmayan, and H. Vaucheret (2012)
PLANT CELL 24, 3603-3612
   Abstract »    Full Text »    PDF »
Failure of the Tomato Trans-Acting Short Interfering RNA Program to Regulate AUXIN RESPONSE FACTOR3 and ARF4 Underlies the Wiry Leaf Syndrome.
T. Yifhar, I. Pekker, D. Peled, G. Friedlander, A. Pistunov, M. Sabban, G. Wachsman, J. P. Alvarez, Z. Amsellem, and Y. Eshed (2012)
PLANT CELL 24, 3575-3589
   Abstract »    Full Text »    PDF »
Widespread dynamic DNA methylation in response to biotic stress.
R. H. Dowen, M. Pelizzola, R. J. Schmitz, R. Lister, J. M. Dowen, J. R. Nery, J. E. Dixon, and J. R. Ecker (2012)
PNAS 109, E2183-E2191
   Abstract »    Full Text »    PDF »
Tobacco calmodulin-like protein provides secondary defense by binding to and directing degradation of virus RNA silencing suppressors.
K. S. Nakahara, C. Masuta, S. Yamada, H. Shimura, Y. Kashihara, T. S. Wada, A. Meguro, K. Goto, K. Tadamura, K. Sueda, et al. (2012)
PNAS 109, 10113-10118
   Abstract »    Full Text »    PDF »
Temperature-Dependent Survival of Turnip Crinkle Virus-Infected Arabidopsis Plants Relies on an RNA Silencing-Based Defense That Requires DCL2, AGO2, and HEN1.
X. Zhang, X. Zhang, J. Singh, D. Li, and F. Qu (2012)
J. Virol. 86, 6847-6854
   Abstract »    Full Text »    PDF »
Differential effects of viral silencing suppressors on siRNA and miRNA loading support the existence of two distinct cellular pools of ARGONAUTE1.
G. Schott, A. Mari-Ordonez, C. Himber, A. Alioua, O. Voinnet, and P. Dunoyer (2012)
EMBO J. 31, 2553-2565
   Abstract »    Full Text »    PDF »
Targeting of Dicer-2 and RNA by a Viral RNA Silencing Suppressor in Drosophila Cells.
N. Qi, L. Zhang, Y. Qiu, Z. Wang, J. Si, Y. Liu, X. Xiang, J. Xie, C.-F. Qin, X. Zhou, et al. (2012)
J. Virol. 86, 5763-5773
   Abstract »    Full Text »    PDF »
Enhanced Susceptibility of Ago1/3 Double-Null Mice to Influenza A Virus Infection.
M. Van Stry, T. H. Oguin III, S. Cheloufi, P. Vogel, M. Watanabe, M. R. Pillai, P. Dash, P. G. Thomas, G. J. Hannon, and M. Bix (2012)
J. Virol. 86, 4151-4157
   Abstract »    Full Text »    PDF »
Deep Sequencing from hen1 Mutants to Identify Small RNA 3' Modifications.
J. Zhai and B. C. Meyers (2012)
Cold Spring Harb Symp Quant Biol 77, 213-219
   Abstract »    Full Text »    PDF »
Satellite RNA-Derived Small Interfering RNA satsiR-12 Targeting the 3' Untranslated Region of Cucumber Mosaic Virus Triggers Viral RNAs for Degradation.
H. Zhu, C.-G. Duan, W.-N. Hou, Q.-S. Du, D.-Q. Lv, R.-X. Fang, and H.-S. Guo (2011)
J. Virol. 85, 13384-13397
   Abstract »    Full Text »    PDF »
RNA-Based Immunity Terminates Viral Infection in Adult Drosophila in the Absence of Viral Suppression of RNA Interference: Characterization of Viral Small Interfering RNA Populations in Wild-Type and Mutant Flies.
Y.-H. Han, Y.-J. Luo, Q. Wu, J. Jovel, X.-H. Wang, R. Aliyari, C. Han, W.-X. Li, and S.-W. Ding (2011)
J. Virol. 85, 13153-13163
   Abstract »    Full Text »    PDF »
The miRNA pathway limits AGO1 availability during siRNA-mediated PTGS defense against exogenous RNA.
A. E. Martinez de Alba, V. Jauvion, A. C. Mallory, N. Bouteiller, and H. Vaucheret (2011)
Nucleic Acids Res. 39, 9339-9344
   Abstract »    Full Text »    PDF »
Intercellular and systemic movement of RNA silencing signals.
C. W. Melnyk, A. Molnar, and D. C. Baulcombe (2011)
EMBO J. 30, 3553-3563
   Abstract »    Full Text »    PDF »
Massive production of small RNAs from a non-coding region of Cauliflower mosaic virus in plant defense and viral counter-defense.
T. Blevins, R. Rajeswaran, M. Aregger, B. K. Borah, M. Schepetilnikov, L. Baerlocher, L. Farinelli, F. Meins Jr, T. Hohn, and M. M. Pooggin (2011)
Nucleic Acids Res. 39, 5003-5014
   Abstract »    Full Text »    PDF »
ARGONAUTE2 Mediates RNA-Silencing Antiviral Defenses against Potato virus X in Arabidopsis.
M. Jaubert, S. Bhattacharjee, A. F. S. Mello, K. L. Perry, and P. Moffett (2011)
Plant Physiology 156, 1556-1564
   Abstract »    Full Text »    PDF »
The specific binding to 21-nt double-stranded RNAs is crucial for the anti-silencing activity of Cucumber vein yellowing virus P1b and perturbs endogenous small RNA populations.
A. Valli, J. C. Oliveros, A. Molnar, D. Baulcombe, and J. A. Garcia (2011)
RNA 17, 1148-1158
   Abstract »    Full Text »    PDF »
The Selaginella Genome Identifies Genetic Changes Associated with the Evolution of Vascular Plants.
J. A. Banks, T. Nishiyama, M. Hasebe, J. L. Bowman, M. Gribskov, C. dePamphilis, V. A. Albert, N. Aono, T. Aoyama, B. A. Ambrose, et al. (2011)
Science 332, 960-963
   Abstract »    Full Text »    PDF »
Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4.
A. Fukudome, A. Kanaya, M. Egami, Y. Nakazawa, A. Hiraguri, H. Moriyama, and T. Fukuhara (2011)
RNA 17, 750-760
   Abstract »    Full Text »    PDF »
The 21-Nucleotide, but Not 22-Nucleotide, Viral Secondary Small Interfering RNAs Direct Potent Antiviral Defense by Two Cooperative Argonautes in Arabidopsis thaliana.
X.-B. Wang, J. Jovel, P. Udomporn, Y. Wang, Q. Wu, W.-X. Li, V. Gasciolli, H. Vaucheret, and S.-W. Ding (2011)
PLANT CELL 23, 1625-1638
   Abstract »    Full Text »    PDF »
Optimal viral strategies for bypassing RNA silencing.
G. Rodrigo, J. Carrera, A. Jaramillo, and S. F. Elena (2011)
J R Soc Interface 8, 257-268
   Abstract »    Full Text »    PDF »
Evolution and Functional Diversification of MIRNA Genes.
J. T. Cuperus, N. Fahlgren, and J. C. Carrington (2011)
PLANT CELL 23, 431-442
   Abstract »    Full Text »    PDF »
Multiple Functions of Rice Dwarf Phytoreovirus Pns10 in Suppressing Systemic RNA Silencing.
B. Ren, Y. Guo, F. Gao, P. Zhou, F. Wu, Z. Meng, C. Wei, and Y. Li (2010)
J. Virol. 84, 12914-12923
   Abstract »    Full Text »    PDF »
RNAi-mediated immunity provides strong protection against the negative-strand RNA vesicular stomatitis virus in Drosophila.
S. Mueller, V. Gausson, N. Vodovar, S. Deddouche, L. Troxler, J. Perot, S. Pfeffer, J. A. Hoffmann, M.-C. Saleh, and J.-L. Imler (2010)
PNAS 107, 19390-19395
   Abstract »    Full Text »    PDF »
Diverging Affinity of Tospovirus RNA Silencing Suppressor Proteins, NSs, for Various RNA Duplex Molecules.
E. Schnettler, H. Hemmes, R. Huismann, R. Goldbach, M. Prins, and R. Kormelink (2010)
J. Virol. 84, 11542-11554
   Abstract »    Full Text »    PDF »
Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome.
S. Laubinger, G. Zeller, S. R. Henz, S. Buechel, T. Sachsenberg, J.-W. Wang, G. Ratsch, and D. Weigel (2010)
PNAS 107, 17466-17473
   Abstract »    Full Text »    PDF »
RNA silencing amplification in plants: Size matters.
R. Schwab and O. Voinnet (2010)
PNAS 107, 14945-14946
   Full Text »    PDF »
22-nucleotide RNAs trigger secondary siRNA biogenesis in plants.
H.-M. Chen, L.-T. Chen, K. Patel, Y.-H. Li, D. C. Baulcombe, and S.-H. Wu (2010)
PNAS 107, 15269-15274
   Abstract »    Full Text »    PDF »
The Capsid Protein of Turnip Crinkle Virus Overcomes Two Separate Defense Barriers To Facilitate Systemic Movement of the Virus in Arabidopsis.
M. Cao, X. Ye, K. Willie, J. Lin, X. Zhang, M. G. Redinbaugh, A. E. Simon, T. J. Morris, and F. Qu (2010)
J. Virol. 84, 7793-7802
   Abstract »    Full Text »    PDF »
The Conserved RNA Trafficking Proteins HPR1 and TEX1 Are Involved in the Production of Endogenous and Exogenous Small Interfering RNA in Arabidopsis.
V. Jauvion, T. Elmayan, and H. Vaucheret (2010)
PLANT CELL 22, 2697-2709
   Abstract »    Full Text »    PDF »
An endogenous, systemic RNAi pathway in plants.
P. Dunoyer, C. A. Brosnan, G. Schott, Y. Wang, F. Jay, A. Alioua, C. Himber, and O. Voinnet (2010)
EMBO J. 29, 1699-1712
   Abstract »    Full Text »    PDF »
Small Silencing RNAs in Plants Are Mobile and Direct Epigenetic Modification in Recipient Cells.
A. Molnar, C. W. Melnyk, A. Bassett, T. J. Hardcastle, R. Dunn, and D. C. Baulcombe (2010)
Science 328, 872-875
   Abstract »    Full Text »    PDF »
Small RNA Duplexes Function as Mobile Silencing Signals Between Plant Cells.
P. Dunoyer, G. Schott, C. Himber, D. Meyer, A. Takeda, J. C. Carrington, and O. Voinnet (2010)
Science 328, 912-916
   Abstract »    Full Text »    PDF »
A viral suppressor protein inhibits host RNA silencing by hooking up with Argonautes.
H. Jin and J. K. Zhu (2010)
Genes & Dev. 24, 853-856
   Abstract »    Full Text »    PDF »
Argonaute quenching and global changes in Dicer homeostasis caused by a pathogen-encoded GW repeat protein.
J. Azevedo, D. Garcia, D. Pontier, S. Ohnesorge, A. Yu, S. Garcia, L. Braun, M. Bergdoll, M. A. Hakimi, T. Lagrange, et al. (2010)
Genes & Dev. 24, 904-915
   Abstract »    Full Text »    PDF »
P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity.
A. Kozlowska-Makulska, H. Guilley, M. S. Szyndel, M. Beuve, O. Lemaire, E. Herrbach, and S. Bouzoubaa (2010)
J. Gen. Virol. 91, 1082-1091
   Abstract »    Full Text »    PDF »
miR390, Arabidopsis TAS3 tasiRNAs, and Their AUXIN RESPONSE FACTOR Targets Define an Autoregulatory Network Quantitatively Regulating Lateral Root Growth.
E. Marin, V. Jouannet, A. Herz, A. S. Lokerse, D. Weijers, H. Vaucheret, L. Nussaume, M. D. Crespi, and A. Maizel (2010)
PLANT CELL 22, 1104-1117
   Abstract »    Full Text »    PDF »
RNA-Dependent RNA Polymerase 6 Delays Accumulation and Precludes Meristem Invasion of a Viroid That Replicates in the Nucleus.
F. Di Serio, A.-E. Martinez de Alba, B. Navarro, A. Gisel, and R. Flores (2010)
J. Virol. 84, 2477-2489
   Abstract »    Full Text »    PDF »
Arabidopsis RNA-Dependent RNA Polymerases and Dicer-Like Proteins in Antiviral Defense and Small Interfering RNA Biogenesis during Turnip Mosaic Virus Infection.
H. Garcia-Ruiz, A. Takeda, E. J. Chapman, C. M. Sullivan, N. Fahlgren, K. J. Brempelis, and J. C. Carrington (2010)
PLANT CELL 22, 481-496
   Abstract »    Full Text »    PDF »
RNAi-mediated viral immunity requires amplification of virus-derived siRNAs in Arabidopsis thaliana.
X.-B. Wang, Q. Wu, T. Ito, F. Cillo, W.-X. Li, X. Chen, J.-L. Yu, and S.-W. Ding (2010)
PNAS 107, 484-489
   Abstract »    Full Text »    PDF »
Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants.
M. G. Lewsey and J. P. Carr (2009)
J. Gen. Virol. 90, 3010-3014
   Abstract »    Full Text »    PDF »
The 2b protein of cucumber mosaic virus is essential for viral infection of the shoot apical meristem and for efficient invasion of leaf primordia in infected tobacco plants.
A. Sunpapao, T. Nakai, F. Dong, T. Mochizuki, and S. T. Ohki (2009)
J. Gen. Virol. 90, 3015-3021
   Abstract »    Full Text »    PDF »
The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes.
A. L. Eamens, N. A. Smith, S. J. Curtin, M.-B. Wang, and P. M. Waterhouse (2009)
RNA 15, 2219-2235
   Abstract »    Full Text »    PDF »
Ectopic 5' Splice Sites Inhibit Gene Expression by Engaging RNA Surveillance and Silencing Pathways in Plants.
K. Wypijewski, C. Hornyik, J. A. Shaw, J. Stephens, R. Goraczniak, S. I. Gunderson, and C. Lacomme (2009)
Plant Physiology 151, 955-965
   Abstract »    Full Text »    PDF »
Clusters and superclusters of phased small RNAs in the developing inflorescence of rice.
C. Johnson, A. Kasprzewska, K. Tennessen, J. Fernandes, G.-L. Nan, V. Walbot, V. Sundaresan, V. Vance, and L. H. Bowman (2009)
Genome Res. 19, 1429-1440
   Abstract »    Full Text »    PDF »
Computational and analytical framework for small RNA profiling by high-throughput sequencing.
N. Fahlgren, C. M. Sullivan, K. D. Kasschau, E. J. Chapman, J. S. Cumbie, T. A. Montgomery, S. D. Gilbert, M. Dasenko, T. W.H. Backman, S. A. Givan, et al. (2009)
RNA 15, 992-1002
   Abstract »    Full Text »    PDF »
ARGONAUTE 1 homeostasis invokes the coordinate action of the microRNA and siRNA pathways.
A. C. Mallory and H. Vaucheret (2009)
EMBO Rep. 10, 521-526
   Abstract »    Full Text »    PDF »
Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection.
H. Senshu, J. Ozeki, K. Komatsu, M. Hashimoto, K. Hatada, M. Aoyama, S. Kagiwada, Y. Yamaji, and S. Namba (2009)
J. Gen. Virol. 90, 1014-1024
   Abstract »    Full Text »    PDF »
Dicing of viral replication intermediates during silencing of latent Drosophila viruses.
A. Flynt, N. Liu, R. Martin, and E. C. Lai (2009)
PNAS 106, 5270-5275
   Abstract »    Full Text »    PDF »
The NS3 protein of rice hoja blanca virus complements the RNAi suppressor function of HIV-1 Tat.
E. Schnettler, W. de Vries, H. Hemmes, J. Haasnoot, R. Kormelink, R. Goldbach, and B. Berkhout (2009)
EMBO Rep. 10, 258-263
   Abstract »    Full Text »    PDF »
Compromised Virus-Induced Gene Silencing in RDR6-Deficient Plants.
F. E. Vaistij and L. Jones (2009)
Plant Physiology 149, 1399-1407
   Abstract »    Full Text »    PDF »
Inhibition of RNA silencing by the coat protein of Pelargonium flower break virus: distinctions from closely related suppressors.
S. Martinez-Turino and C. Hernandez (2009)
J. Gen. Virol. 90, 519-525
   Abstract »    Full Text »    PDF »
The evolution of RNAi as a defence against viruses and transposable elements.
D. J Obbard, K. H.J Gordon, A. H Buck, and F. M Jiggins (2009)
Phil Trans R Soc B 364, 99-115
   Abstract »    Full Text »    PDF »
AGO1-miR173 complex initiates phased siRNA formation in plants.
T. A. Montgomery, S. J. Yoo, N. Fahlgren, S. D. Gilbert, M. D. Howell, C. M. Sullivan, A. Alexander, G. Nguyen, E. Allen, J. H. Ahn, et al. (2008)
PNAS 105, 20055-20062
   Abstract »    Full Text »    PDF »
Artificial MicroRNAs Highly Accessible to Targets Confer Efficient Virus Resistance in Plants.
C.-G. Duan, C.-H. Wang, R.-X. Fang, and H.-S. Guo (2008)
J. Virol. 82, 11084-11095
   Abstract »    Full Text »    PDF »
Evolution of Arabidopsis MIR genes generates novel microRNA classes.
F. Vazquez, T. Blevins, J. Ailhas, T. Boller, and F. Meins Jr (2008)
Nucleic Acids Res. 36, 6429-6438
   Abstract »    Full Text »    PDF »
The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis.
P. V. Shivaprasad, R. Rajeswaran, T. Blevins, J. Schoelz, F. Meins Jr, T. Hohn, and M. M. Pooggin (2008)
Nucleic Acids Res. 36, 5896-5909
   Abstract »    Full Text »    PDF »
From Hypo- to Hypersuppression: Effect of Amino Acid Substitutions on the RNA-Silencing Suppressor Activity of the Tobacco etch potyvirus HC-Pro.
C. Torres-Barcelo, S. Martin, J.-A. Daros, and S. F. Elena (2008)
Genetics 180, 1039-1049
   Abstract »    Full Text »    PDF »
Distinct size distribution of endogenous siRNAs in maize: Evidence from deep sequencing in the mop1-1 mutant.
K. Nobuta, C. Lu, R. Shrivastava, M. Pillay, E. De Paoli, M. Accerbi, M. Arteaga-Vazquez, L. Sidorenko, D.-H. Jeong, Y. Yen, et al. (2008)
PNAS 105, 14958-14963
   Abstract »    Full Text »    PDF »
Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1.
F. Qu, X. Ye, and T. J. Morris (2008)
PNAS 105, 14732-14737
   Abstract »    Full Text »    PDF »
Hibiscus chlorotic ringspot virus coat protein inhibits trans-acting small interfering RNA biogenesis in Arabidopsis.
C. Meng, J. Chen, S.-w. Ding, J. Peng, and S.-M. Wong (2008)
J. Gen. Virol. 89, 2349-2358
   Abstract »    Full Text »    PDF »
Nuclear import of CaMV P6 is required for infection and suppression of the RNA silencing factor DRB4.
G. Haas, J. Azevedo, G. Moissiard, A. Geldreich, C. Himber, M. Bureau, T. Fukuhara, M. Keller, and O. Voinnet (2008)
EMBO J. 27, 2102-2112
   Abstract »    Full Text »    PDF »
Conifers have a unique small RNA silencing signature.
E. V. Dolgosheina, R. D. Morin, G. Aksay, S. C. Sahinalp, V. Magrini, E. R. Mardis, J. Mattsson, and P. J. Unrau (2008)
RNA 14, 1508-1515
   Abstract »    Full Text »    PDF »
Virus meets RNAi: Symposium on Antiviral Applications of RNA Interference.
R. P. van Rij (2008)
EMBO Rep. 9, 725-729
   Full Text »    PDF »
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1.
Z. Dong, M.-H. Han, and N. Fedoroff (2008)
PNAS 105, 9970-9975
   Abstract »    Full Text »    PDF »
Inhibition of 3' modification of small RNAs in virus-infected plants require spatial and temporal co-expression of small RNAs and viral silencing-suppressor proteins.
R. Lozsa, T. Csorba, L. Lakatos, and J. Burgyan (2008)
Nucleic Acids Res. 36, 4099-4107
   Abstract »    Full Text »    PDF »
Effects of viral silencing suppressors on tobacco ringspot virus infection in two Nicotiana species.
S. A. Siddiqui, C. Sarmiento, M. Kiisma, S. Koivumaki, A. Lemmetty, E. Truve, and K. Lehto (2008)
J. Gen. Virol. 89, 1502-1508
   Abstract »    Full Text »    PDF »
Structural and Genetic Requirements for the Biogenesis of Tobacco Rattle Virus-Derived Small Interfering RNAs.
L. Donaire, D. Barajas, B. Martinez-Garcia, L. Martinez-Priego, I. Pagan, and C. Llave (2008)
J. Virol. 82, 5167-5177
   Abstract »    Full Text »    PDF »
Hairpin RNAs derived from RNA polymerase II and polymerase III promoter-directed transgenes are processed differently in plants.
M.-B. Wang, C. A. Helliwell, L.-M. Wu, P. M. Waterhouse, W. J. Peacock, and E. S. Dennis (2008)
RNA 14, 903-913
   Abstract »    Full Text »    PDF »
Activity Range of Arabidopsis Small RNAs Derived from Different Biogenesis Pathways.
E. M. Tretter, J. P. Alvarez, Y. Eshed, and J. L. Bowman (2008)
Plant Physiology 147, 58-62
   Full Text »    PDF »
RNAs as extracellular signaling molecules.
M. E Dinger, T. R Mercer, and J. S Mattick (2008)
J. Mol. Endocrinol. 40, 151-159
   Abstract »    Full Text »    PDF »

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