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Science 313 (5786): 533-536

Copyright © 2006 by the American Association for the Advancement of Science

Activated Signal Transduction Kinases Frequently Occupy Target Genes

Dmitry K. Pokholok,1* Julia Zeitlinger,1* Nancy M. Hannett,1 David B. Reynolds,1 Richard A. Young1,2{dagger}

Abstract: Cellular signal transduction pathways modify gene expression programs in response to changes in the environment, but the mechanisms by which these pathways regulate populations of genes under their control are not entirely understood. We present evidence that most mitogen-activated protein kinases and protein kinase A subunits become physically associated with the genes that they regulate in the yeast (Saccharomyces cerevisiae) genome. The ability to detect this interaction of signaling kinases with target genes can be used to more precisely and comprehensively map the regulatory circuitry that eukaryotic cells use to respond to their environment.

1 Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
2 Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: young{at}

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