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Science 313 (5788): 851-853

Copyright © 2006 by the American Association for the Advancement of Science

Tequila, a Neurotrypsin Ortholog, Regulates Long-Term Memory Formation in Drosophila

Gérard Didelot,1,2* Florence Molinari,3 Paul Tchénio,4 Daniel Comas,2{dagger} Elodie Milhiet,4 Arnold Munnich,3 Laurence Colleaux,3 Thomas Preat1,2{ddagger}§

Abstract: Mutations in the human neurotrypsin gene are associated with autosomal recessive mental retardation. To further understand the pathophysiological consequences of the lack of this serine protease, we studied Tequila (Teq), the Drosophila neurotrypsin ortholog, using associative memory as a behavioral readout. We found that teq inactivation resulted in a long-term memory (LTM)–specific defect. After LTM conditioning of wild-type flies, teq expression transiently increased in the mushroom bodies. Moreover, specific inhibition of teq expression in adult mushroom bodies resulted in a reversible LTM defect. Hence, the Teq pathway is essential for information processing in Drosophila.

1 Gènes et Dynamique des Systèmes de Mémoire, UMR CNRS 7637, Ecole Supérieure de Physique et de Chimie Industrielles, 10 Rue Vauquelin 75005 Paris, France.
2 Développement, Evolution et Plasticité du Système Nerveux, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
3 Département de Génétique et Unité de Recherche sur les Handicaps Génétiques de l'Enfant, INSERM U781, Hôpital Necker-Enfants Malades, 149 Rue de Sèvres, 75743 Paris Cedex 15, France.
4 Laboratoire Aimé Cotton, UPR CNRS 3321, Bâtiment 505, 91405 Orsay Cedex, France.

* Present address: Center for Integrative Genomics, University of Lausanne, CH-1015 Lausanne, Switzerland.

{dagger} Present address: Glaxo Smith Kline R&D, Biology Department, 25 Avenue du Québec, 91195 Les Ulis, France.

{ddagger} Present address: Gènes et Dynamique des Systèmes de Mémoire, UMR CNRS 7637, ESPCI, 10 Rue Vauquelin 75005 Paris, France.

§ To whom correspondence should be addressed. E-mail: thomas.preat{at}

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