Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 313 (5788): 851-853

Copyright © 2006 by the American Association for the Advancement of Science

Tequila, a Neurotrypsin Ortholog, Regulates Long-Term Memory Formation in Drosophila

Gérard Didelot,1,2* Florence Molinari,3 Paul Tchénio,4 Daniel Comas,2{dagger} Elodie Milhiet,4 Arnold Munnich,3 Laurence Colleaux,3 Thomas Preat1,2{ddagger}§

Abstract: Mutations in the human neurotrypsin gene are associated with autosomal recessive mental retardation. To further understand the pathophysiological consequences of the lack of this serine protease, we studied Tequila (Teq), the Drosophila neurotrypsin ortholog, using associative memory as a behavioral readout. We found that teq inactivation resulted in a long-term memory (LTM)–specific defect. After LTM conditioning of wild-type flies, teq expression transiently increased in the mushroom bodies. Moreover, specific inhibition of teq expression in adult mushroom bodies resulted in a reversible LTM defect. Hence, the Teq pathway is essential for information processing in Drosophila.

1 Gènes et Dynamique des Systèmes de Mémoire, UMR CNRS 7637, Ecole Supérieure de Physique et de Chimie Industrielles, 10 Rue Vauquelin 75005 Paris, France.
2 Développement, Evolution et Plasticité du Système Nerveux, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
3 Département de Génétique et Unité de Recherche sur les Handicaps Génétiques de l'Enfant, INSERM U781, Hôpital Necker-Enfants Malades, 149 Rue de Sèvres, 75743 Paris Cedex 15, France.
4 Laboratoire Aimé Cotton, UPR CNRS 3321, Bâtiment 505, 91405 Orsay Cedex, France.

* Present address: Center for Integrative Genomics, University of Lausanne, CH-1015 Lausanne, Switzerland.

{dagger} Present address: Glaxo Smith Kline R&D, Biology Department, 25 Avenue du Québec, 91195 Les Ulis, France.

{ddagger} Present address: Gènes et Dynamique des Systèmes de Mémoire, UMR CNRS 7637, ESPCI, 10 Rue Vauquelin 75005 Paris, France.

§ To whom correspondence should be addressed. E-mail: thomas.preat{at}espci.fr


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Patterns of Positive Selection in Seven Ant Genomes.
J. Roux, E. Privman, S. Moretti, J. T. Daub, M. Robinson-Rechavi, and L. Keller (2014)
Mol. Biol. Evol. 31, 1661-1685
   Abstract »    Full Text »    PDF »
Presenilins Regulate Neurotrypsin Gene Expression and Neurotrypsin-dependent Agrin Cleavage via Cyclic AMP Response Element-binding Protein (CREB) Modulation.
A. Almenar-Queralt, S. N. Kim, C. Benner, C. M. Herrera, D. E. Kang, I. Garcia-Bassets, and L. S. B. Goldstein (2013)
J. Biol. Chem. 288, 35222-35236
   Abstract »    Full Text »    PDF »
To Favor Survival Under Food Shortage, the Brain Disables Costly Memory.
P.-Y. Placais and T. Preat (2013)
Science 339, 440-442
   Abstract »    Full Text »    PDF »
Identification of Gene Expression Changes Associated With Long-Term Memory of Courtship Rejection in Drosophila Males.
A. Winbush, D. Reed, P. L. Chang, S. V. Nuzhdin, L. C. Lyons, and M. N. Arbeitman (2012)
g3 2, 1437-1445
   Abstract »    Full Text »    PDF »
Deep sequencing the circadian and diurnal transcriptome of Drosophila brain.
M. E. Hughes, G. R. Grant, C. Paquin, J. Qian, and M. N. Nitabach (2012)
Genome Res. 22, 1266-1281
   Abstract »    Full Text »    PDF »
Visualizing Long-Term Memory Formation in Two Neurons of the Drosophila Brain.
C.-C. Chen, J.-K. Wu, H.-W. Lin, T.-P. Pai, T.-F. Fu, C.-L. Wu, T. Tully, and A.-S. Chiang (2012)
Science 335, 678-685
   Abstract »    Full Text »    PDF »
Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants.
Z. Guan, L. K. Buhl, W. G. Quinn, and J. T. Littleton (2011)
Learn. Mem. 18, 191-206
   Abstract »    Full Text »    PDF »
Drosophila Orb2 targets genes involved in neuronal growth, synapse formation, and protein turnover.
T. Mastushita-Sakai, E. White-Grindley, J. Samuelson, C. Seidel, and K. Si (2010)
PNAS 107, 11987-11992
   Abstract »    Full Text »    PDF »
ben Functions with Scamp during Synaptic Transmission and Long-Term Memory Formation in Drosophila.
H. Zhao, X. Zheng, X. Yuan, L. Wang, X. Wang, Y. Zhong, Z. Xie, and T. Tully (2009)
J. Neurosci. 29, 414-424
   Abstract »    Full Text »    PDF »
nemy encodes a cytochrome b561 that is required for Drosophila learning and memory.
K. G. Iliadi, A. Avivi, N. N. Iliadi, D. Knight, A. B. Korol, E. Nevo, P. Taylor, M. F. Moran, N. G. Kamyshev, and G. L. Boulianne (2008)
PNAS 105, 19986-19991
   Abstract »    Full Text »    PDF »
Pleiotropic Effects of Drosophila neuralized on Complex Behaviors and Brain Structure.
S. M. Rollmann, L. Zwarts, A. C. Edwards, A. Yamamoto, P. Callaerts, K. Norga, T. F. C. Mackay, and R. R. H. Anholt (2008)
Genetics 179, 1327-1336
   Abstract »    Full Text »    PDF »
Rapid Consolidation to a radish and Protein Synthesis-Dependent Long-Term Memory after Single-Session Appetitive Olfactory Conditioning in Drosophila.
M. J. Krashes and S. Waddell (2008)
J. Neurosci. 28, 3103-3113
   Abstract »    Full Text »    PDF »
Comment on "Tequila, a Neurotrypsin Ortholog, Regulates Long-Term Memory Formation in Drosophila".
P. Sonderegger and L. Patthy (2007)
Science 316, 1698b
   Abstract »    Full Text »    PDF »
Response to Comment on "Tequila, a Neurotrypsin Ortholog, Regulates Long-Term Memory Formation in Drosophila".
T. Preat, J.-L. Da Lage, L. Colleaux, G. Didelot, F. Molinari, P. Tchenio, E. Milhiet, A. Munnich, and M.-L. Cariou (2007)
Science 316, 1698c
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882