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Science 314 (5799): 654-657

Copyright © 2006 by the American Association for the Advancement of Science

A Centrosome-Independent Role for {gamma}-TuRC Proteins in the Spindle Assembly Checkpoint

Hannah Müller, Marie-Laure Fogeron, Verena Lehmann, Hans Lehrach, Bodo M. H. Lange*

Abstract: The spindle assembly checkpoint guards the fidelity of chromosome segregation. It requires the close cooperation of cell cycle regulatory proteins and cytoskeletal elements to sense spindle integrity. The role of the centrosome, the organizing center of the microtubule cytoskeleton, in the spindle checkpoint is unclear. We found that the molecular requirements for a functional spindle checkpoint included components of the large {gamma}-tubulin ring complex ({gamma}-TuRC). However, their localization at the centrosome and centrosome integrity were not essential for this function. Thus, the spindle checkpoint can be activated at the level of microtubule nucleation.

Department of Vertebrate Genomics, Max-Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany.

* To whom correspondence should be addressed. E-mail: lange_b{at}molgen.mpg.de

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