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Science 315 (5808): 101-104

Copyright © 2007 by the American Association for the Advancement of Science

A Cytokinin Perception Mutant Colonized by Rhizobium in the Absence of Nodule Organogenesis

Jeremy D. Murray,1 Bogumil J. Karas,1 Shusei Sato,2 Satoshi Tabata,2 Lisa Amyot,1 Krzysztof Szczyglowski1*

Abstract: In legumes, Nod-factor signaling by rhizobia initiates the development of the nitrogen-fixing nodule symbiosis, but the direct cell division stimulus that brings about nodule primordia inception in the root cortex remains obscure. We showed that Lotus japonicus plants homozygous for a mutation in the HYPERINFECTED 1 (HIT1) locus exhibit abundant infection-thread formation but fail to initiate timely cortical cell divisions in response to rhizobial signaling. We demonstrated that the corresponding gene encodes a cytokinin receptor that is required for the activation of the nodule inception regulator Nin and nodule organogenesis.

1 Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford Street, London, Ontario N5V 4T3, Canada.
2 Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0812, Japan.

* To whom correspondence should be addressed. E-mail: szczyglowskik{at}agr.gc.ca

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