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Science 315 (5817): 1405-1408

Copyright © 2007 by the American Association for the Advancement of Science

Dynamics of Replication-Independent Histone Turnover in Budding Yeast

Michael F. Dion,1*{dagger} Tommy Kaplan,2,3* Minkyu Kim,4 Stephen Buratowski,4 Nir Friedman,2 Oliver J. Rando1{dagger}{ddagger}

Abstract: Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% of the genome, and at lower (~265 base pair) resolution over the entire genome. We find that nucleosomes at promoters are replaced more rapidly than at coding regions and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.

1 Faculty of Arts and Sciences, Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.
2 School of Computer Science and Engineering, The Hebrew University, Jerusalem 91904, Israel.
3 Department of Molecular Genetics and Biotechnology, Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel.
4 Department of Biological Chemistry and Molecular Pharmacology, Harvard University, 240 Longwood Avenue, Boston, MA 02115, USA.

* These authors contributed equally to this work.

{dagger} Present address: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.

{ddagger} To whom correspondence should be addressed. E-mail: Oliver.Rando{at}umassmed.edu

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