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Science 315 (5817): 1411-1415

Copyright © 2007 by the American Association for the Advancement of Science

Anaphase Onset Before Complete DNA Replication with Intact Checkpoint Responses

Jordi Torres-Rosell,1*{dagger} Giacomo De Piccoli,1* Violeta Cordon-Preciado,1 Sarah Farmer,1 Adam Jarmuz,1 Felix Machin,1{ddagger} Philippe Pasero,2 Michael Lisby,3 James E. Haber,4 Luis Aragón1§

Abstract: Cellular checkpoints prevent mitosis in the presence of stalled replication forks. Whether checkpoints also ensure the completion of DNA replication before mitosis is unknown. Here, we show that in yeast smc5-smc6 mutants, which are related to cohesin and condensin, replication is delayed, most significantly at natural replication-impeding loci like the ribosomal DNA gene cluster. In the absence of Smc5-Smc6, chromosome nondisjunction occurs as a consequence of mitotic entry with unfinished replication despite intact checkpoint responses. Eliminating processes that obstruct replication fork progression restores the temporal uncoupling between replication and segregation in smc5-smc6 mutants. We propose that the completion of replication is not under the surveillance of known checkpoints.

1 Cell Cycle Group, Medical Research Council (MRC) Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK.
2 Institute of Human Genetics, CNRS Unité Propre de Recherche 1142, 141 Rue de la Cardonille, 34396 Montpellier, France.
3 Department of Genetics, Institute of Molecular Biology and Physiology, University of Copenhagen, Øster Farimagsgade 2A, DK-1353 Copenhagen, Denmark.
4 Rosenstiel Center, Brandeis University, 415 South Street, Mail Stop 029, Waltham, MA 02454–9110, USA.

* These authors contributed equally to this work.

{dagger} Present address: Departament Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Montserrat Roig 2, 25008 Lleida, Spain.

{ddagger} Present address: Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Carretera del Rosario sin número, 38010, Santa Cruz de Tenerife, Spain.

§ To whom correspondence should be addressed. E-mail: luis.aragon{at}

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Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells.
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   Abstract »    Full Text »    PDF »
MRX protects fork integrity at protein-DNA barriers, and its absence causes checkpoint activation dependent on chromatin context.
I. B. Bentsen, I. Nielsen, M. Lisby, H. B. Nielsen, S. S. Gupta, K. Mundbjerg, A. H. Andersen, and L. Bjergbaek (2013)
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   Abstract »    Full Text »    PDF »
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H. Hua, M. Namdar, O. Ganier, J. Gregan, M. Mechali, and S. E. Kearsey (2013)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
Cohesin-SA1 deficiency drives aneuploidy and tumourigenesis in mice due to impaired replication of telomeres.
S. Remeseiro, A. Cuadrado, M. Carretero, P. Martinez, W. C. Drosopoulos, M. Canamero, C. L. Schildkraut, M. A. Blasco, and A. Losada (2012)
EMBO J. 31, 2076-2089
   Abstract »    Full Text »    PDF »
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L. J. Reha-Krantz, M. S. P. Siddique, K. Murphy, A. Tam, M. O'Carroll, S. Lou, A. Schultz, and C. Boone (2011)
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   Abstract »    Full Text »    PDF »
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S. Sofueva, F. Osman, A. Lorenz, R. Steinacher, S. Castagnetti, J. Ledesma, and M. C. Whitby (2011)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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J. A. Vaisica, A. Baryshnikova, M. Costanzo, C. Boone, and G. W. Brown (2011)
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   Abstract »    Full Text »    PDF »
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R. Rai, S. P. M. V. Varma, N. Shinde, S. Ghosh, S. P. Kumaran, G. Skariah, and S. Laloraya (2011)
J. Biol. Chem. 286, 14516-14530
   Abstract »    Full Text »    PDF »
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   Abstract »    PDF »
Roles of Vertebrate Smc5 in Sister Chromatid Cohesion and Homologous Recombinational Repair.
A. K. Stephan, M. Kliszczak, H. Dodson, C. Cooley, and C. G. Morrison (2011)
Mol. Cell. Biol. 31, 1369-1381
   Abstract »    Full Text »    PDF »
The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages.
M. Bermudez-Lopez, A. Ceschia, G. de Piccoli, N. Colomina, P. Pasero, L. Aragon, and J. Torres-Rosell (2010)
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   Abstract »    Full Text »    PDF »
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A. Chavez, V. George, V. Agrawal, and F. B. Johnson (2010)
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   Abstract »    Full Text »    PDF »
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S. Dulev, C. de Renty, R. Mehta, I. Minkov, E. Schwob, and A. Strunnikov (2009)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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M. P. Andersen, Z. W. Nelson, E. D. Hetrick, and D. E. Gottschling (2008)
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   Abstract »    Full Text »    PDF »
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   Full Text »    PDF »
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