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Science 315 (5819): 1712-1716

Copyright © 2007 by the American Association for the Advancement of Science

A G Protein-Coupled Receptor Is a Plasma Membrane Receptor for the Plant Hormone Abscisic Acid

Xigang Liu,1,2 Yanling Yue,1 Bin Li,3 Yanli Nie,1 Wei Li,2 Wei-Hua Wu,3 Ligeng Ma1,2*

Abstract: The plant hormone abscisic acid (ABA) regulates many physiological and developmental processes in plants. The mechanism of ABA perception at the cell surface is not understood. Here, we report that a G protein–coupled receptor genetically and physically interacts with the G protein {alpha} subunit GPA1 to mediate all known ABA responses in Arabidopsis. Overexpressing this receptor results in an ABA-hypersensitive phenotype. This receptor binds ABA with high affinity at physiological concentration with expected kinetics and stereospecificity. The binding of ABA to the receptor leads to the dissociation of the receptor-GPA1 complex in yeast. Our results demonstrate that this G protein–coupled receptor is a plasma membrane ABA receptor.

1 National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China.
2 Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, Hebei 050016, China.
3 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.

* To whom correspondence should be addressed. E-mail: maligeng{at}nibs.ac.cn


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