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Science 316 (5823): 404-405

Copyright © 2007 by the American Association for the Advancement of Science

Male Germline Stem Cells: From Mice to Men

Ralph L. Brinster

Abstract: The production of functional male gametes is dependent on the continuous activity of germline stem cells. The availability of a transplantation assay system to unequivocally identify male germline stem cells has allowed their in vitro culture, cryopreservation, and genetic modification. Moreover, the system has enabled the identification of conditions and factors involved in stem cell self-renewal, the foundation of spermatogenesis, and the production of spermatozoa. The increased knowledge about these cells is also of great potential practical value, for example, for the possible cryopreservation of stem cells from boys undergoing treatment for cancer to safeguard their germ line.

Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Purification of Oogonial Stem Cells From Adult Mouse and Human Ovaries: An Assessment of the Literature and a View Toward the Future.
D. C. Woods, Y. A. R. White, and J. L. Tilly (2013)
Reproductive Sciences 20, 7-15
   Abstract »    Full Text »    PDF »
Fertile offspring derived from mouse spermatogonial stem cells cryopreserved for more than 14 years.
X. Wu, S. M. Goodyear, L. K. Abramowitz, M. S. Bartolomei, J. W. Tobias, M. R. Avarbock, and R. L. Brinster (2012)
Hum. Reprod. 27, 1249-1259
   Abstract »    Full Text »    PDF »
The Germline Stem Cell Niche Unit in Mammalian Testes.
J. M. Oatley and R. L. Brinster (2012)
Physiol Rev 92, 577-595
   Abstract »    Full Text »    PDF »
Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions.
B. P. Hermann, M. Sukhwani, J. Salati, Y. Sheng, T. Chu, and K. E. Orwig (2011)
Hum. Reprod. 26, 3222-3231
   Abstract »    Full Text »    PDF »
Spermatogonial Stem Cell Self-Renewal Requires ETV5-Mediated Downstream Activation of Brachyury in Mice.
X. Wu, S. M. Goodyear, J. W. Tobias, M. R. Avarbock, and R. L. Brinster (2011)
Biol Reprod 85, 1114-1123
   Abstract »    Full Text »    PDF »
Sperm storage for cancer patients in the UK: a review of current practice.
V. Sharma (2011)
Hum. Reprod. 26, 2935-2943
   Abstract »    Full Text »    PDF »
MicroRNA-21 regulates the self-renewal of mouse spermatogonial stem cells.
Z. Niu, S. M. Goodyear, S. Rao, X. Wu, J. W. Tobias, M. R. Avarbock, and R. L. Brinster (2011)
PNAS 108, 12740-12745
   Abstract »    Full Text »    PDF »
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
H. Kubota, X. Wu, S. M. Goodyear, M. R. Avarbock, and R. L. Brinster (2011)
FASEB J 25, 2604-2614
   Abstract »    Full Text »    PDF »
Improved In Vitro Culture Conditions to Enhance the Survival, Mitotic Activity, and Transplantability of Rainbow Trout Type A Spermatogonia.
S. Shikina and G. Yoshizaki (2010)
Biol Reprod 83, 268-276
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Loss of protein phosphatase 1c{gamma} (PPP1CC) leads to impaired spermatogenesis associated with defects in chromatin condensation and acrosome development: an ultrastructural analysis.
N. Forgione, A. W. Vogl, and S. Varmuza (2010)
Reproduction 139, 1021-1029
   Abstract »    Full Text »    PDF »
NANOS: A Germline Stem Cell's Guardian Angel.
R. Shen and T. Xie (2010)
J Mol Cell Biol 2, 76-77
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Spermatogonial stem cells in higher primates: are there differences from those in rodents?.
B. P Hermann, M. Sukhwani, M. C Hansel, and K. E Orwig (2010)
Reproduction 139, 479-493
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An experimental protocol for fertility preservation in prepubertal boys recently diagnosed with cancer: a report of acceptability and safety.
J.P. Ginsberg, C.A. Carlson, K. Lin, W.L. Hobbie, E. Wigo, X. Wu, R.L. Brinster, and T.F. Kolon (2010)
Hum. Reprod. 25, 37-41
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Recent developments in testis tissue xenografting.
J. R Rodriguez-Sosa and I. Dobrinski (2009)
Reproduction 138, 187-194
   Abstract »    Full Text »    PDF »
Spermatogonial Stem Cells Derived from Infertile Wv/Wv Mice Self-Renew In Vitro and Generate Progeny Following Transplantation.
H. Kubota, M. R. Avarbock, J. A. Schmidt, and R. L. Brinster (2009)
Biol Reprod 81, 293-301
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Molecular dissection of the male germ cell lineage identifies putative spermatogonial stem cells in rhesus macaques.
B. P. Hermann, M. Sukhwani, D. R. Simorangkir, T. Chu, T. M. Plant, and K. E. Orwig (2009)
Hum. Reprod. 24, 1704-1716
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The Molecular Signature of Spermatogonial Stem/Progenitor Cells in the 6-Day-Old Mouse Testis.
M. Kokkinaki, T.-L. Lee, Z. He, J. Jiang, N. Golestaneh, M.-C. Hofmann, W.-Y. Chan, and M. Dym (2009)
Biol Reprod 80, 707-717
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Autologous spermatogonial stem cell transplantation in man: current obstacles for a future clinical application.
M. Geens, E. Goossens, G. De Block, L. Ning, D. Van Saen, and H. Tournaye (2008)
Hum. Reprod. Update 14, 121-130
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Cryopreservation of the Germplasm of Animals Used in Biological and Medical Research: Importance, Impact, Status, and Future Directions.
P. Mazur, S.P Leibo, and G. E Seidel Jr. (2008)
Biol Reprod 78, 2-12
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Review Article: Stem Cells in Human Reproduction.
C. E. Gargett (2007)
Reproductive Sciences 14, 405-424
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Male and Female Drosophila Germline Stem Cells: Two Versions of Immortality.
M. T. Fuller and A. C. Spradling (2007)
Science 316, 402-404
   Abstract »    Full Text »    PDF »

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