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Science 316 (5832): 1753-1756

Copyright © 2007 by the American Association for the Advancement of Science

Quantitative Morphological Signatures Define Local Signaling Networks Regulating Cell Morphology

Chris Bakal,1,2,3*{dagger} John Aach,1* George Church,1 Norbert Perrimon1,2

Abstract: Although classical genetic and biochemical approaches have identified hundreds of proteins that function in the dynamic remodeling of cell shape in response to upstream signals, there is currently little systems-level understanding of the organization and composition of signaling networks that regulate cell morphology. We have developed quantitative morphological profiling methods to systematically investigate the role of individual genes in the regulation of cell morphology in a fast, robust, and cost-efficient manner. We analyzed a compendium of quantitative morphological signatures and described the existence of local signaling networks that act to regulate cell protrusion, adhesion, and tension.

1 Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
2 Howard Hughes Medical Institute, Boston, MA 02215, USA.
3 MIT Computer Science and Technology Laboratory, Cambridge, MA 02139, USA.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed.

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