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Science 318 (5850): 645-648

Copyright © 2007 by the American Association for the Advancement of Science

Plant Pathogen Recognition Mediated by Promoter Activation of the Pepper Bs3 Resistance Gene

Patrick Römer, Simone Hahn, Tina Jordan,* Tina Strauß, Ulla Bonas, Thomas Lahaye{dagger}

Abstract: Plant disease resistance (R) proteins recognize matching pathogen avirulence proteins. Alleles of the pepper R gene Bs3 mediate recognition of the Xanthomonas campestris pv. vesicatoria (Xcv) type III effector protein AvrBs3 and its deletion derivative AvrBs3{Delta}rep16. Pepper Bs3 and its allelic variant Bs3-E encode flavin monooxygenases with a previously unknown structure and are transcriptionally activated by the Xcv effector proteins AvrBs3 and AvrBs3{Delta}rep16, respectively. We found that recognition specificity resides in the Bs3 and Bs3-E promoters and is determined by binding of AvrBs3 or AvrBs3{Delta}rep16 to a defined promoter region. Our data suggest a recognition mechanism in which the Avr protein binds and activates the promoter of the cognate R gene.

Institute of Biology, Department of Genetics, Martin-Luther-University Halle-Wittenberg, D-06099 Halle (Saale), Germany.

* Present address: Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, 8008 Zürich, Switzerland.

{dagger} To whom correspondence should be addressed. E-mail: lahaye{at}genetik.uni-halle.de

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