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Science 318 (5857): 1789-1792

Copyright © 2007 by the American Association for the Advancement of Science

The Arabidopsis Circadian Clock Incorporates a cADPR-Based Feedback Loop

Antony N. Dodd,1 Michael J. Gardner,1 Carlos T. Hotta,1 Katharine E. Hubbard,1 Neil Dalchau,1 John Love,1* Jean-Maurice Assie,1 Fiona C. Robertson,1 Mia Kyed Jakobsen,1{dagger} Jorge Gonçalves,2 Dale Sanders,3 Alex A. R. Webb1{ddagger}

Abstract: Transcriptional feedback loops are a feature of circadian clocks in both animals and plants. We show that the plant circadian clock also incorporates the cytosolic signaling molecule cyclic adenosine diphosphate ribose (cADPR). cADPR modulates the circadian oscillator's transcriptional feedback loops and drives circadian oscillations of Ca2+ release. The effects of antagonists of cADPR signaling, manipulation of cADPR synthesis, and mathematical simulation of the interaction of cADPR with the circadian clock indicate that cADPR forms a feedback loop within the plant circadian clock.

1 Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
2 Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK.
3 Department of Biology, University of York, York YO10 5YW, UK.

* Present address: School of Biosciences, Geoffrey Pope Building, University of Exeter EX4 4QD, UK.

{dagger} Present address: Institute for Plant Biology, Copenhagen University, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark.

{ddagger} To whom correspondence should be addressed. E-mail: alex.webb{at}

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