Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 320 (5874): 382-385

Copyright © 2008 by the American Association for the Advancement of Science

Divergence of Quaternary Structures Among Bacterial Flagellar Filaments

Vitold E. Galkin,1 Xiong Yu,1 Jakub Bielnicki,1 John Heuser,2 Cheryl P. Ewing,3 Patricia Guerry,3 Edward H. Egelman1*

Abstract: It has been widely assumed that the atomic structure of the flagellar filament from Salmonella typhimurium serves as a model for all bacterial flagellar filaments given the sequence conservation in the coiled-coil regions responsible for polymerization. On the basis of electron microscopic images, we show that the flagellar filaments from Campylobacter jejuni have seven protofilaments rather than the 11 in S. typhimurium. The vertebrate Toll-like receptor 5 (TLR5) recognizes a region of bacterial flagellin that is involved in subunit-subunit assembly in Salmonella and many other pathogenic bacteria, and this short region has diverged in Campylobacter and related bacteria, such as Helicobacter pylori, which are not recognized by TLR5. The driving force in the change of quaternary structure between Salmonella and Campylobacter may have been the evasion of TLR5.

1 Department of Biochemistry and Molecular Genetics, Box 800733, University of Virginia, Charlottesville, VA 22908–0733, USA.
2 Department of Cell Biology, Washington University School of Medicine, St. Louis, MO 63110, USA.
3 Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.

* To whom correspondence should be addressed. E-mail: egelman{at}virginia.edu


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Polysaccharide Capsule of Campylobacter jejuni Modulates the Host Immune Response.
A. C. Maue, K. L. Mohawk, D. K. Giles, F. Poly, C. P. Ewing, Y. Jiao, G. Lee, Z. Ma, M. A. Monteiro, C. L. Hill, et al. (2013)
Infect. Immun. 81, 665-672
   Abstract »    Full Text »    PDF »
Two Genera of Magnetococci with Bean-like Morphology from Intertidal Sediments of the Yellow Sea, China.
W.-Y. Zhang, K. Zhou, H.-M. Pan, H.-D. Yue, M. Jiang, T. Xiao, and L.-F. Wu (2012)
Appl. Envir. Microbiol. 78, 5606-5611
   Abstract »    Full Text »    PDF »
Structural diversity of bacterial flagellar motors.
S. Chen, M. Beeby, G. E. Murphy, J. R. Leadbetter, D. R. Hendrixson, A. Briegel, Z. Li, J. Shi, E. I. Tocheva, A. Muller, et al. (2011)
EMBO J. 30, 2972-2981
   Abstract »    Full Text »    PDF »
Change Is Good: Variations in Common Biological Mechanisms in the Epsilonproteobacterial Genera Campylobacter and Helicobacter.
J. J. Gilbreath, W. L. Cody, D. S. Merrell, and D. R. Hendrixson (2011)
Microbiol. Mol. Biol. Rev. 75, 84-132
   Abstract »    Full Text »    PDF »
Molecular interaction of flagellar export chaperone FliS and cochaperone HP1076 in Helicobacter pylori.
W. W. L. Lam, E. J. Woo, M. Kotaka, W. K. Tam, Y. C. Leung, T. K. W. Ling, and S. W. N. Au (2010)
FASEB J 24, 4020-4032
   Abstract »    Full Text »    PDF »
Force-dependent polymorphism in type IV pili reveals hidden epitopes.
N. Biais, D. L. Higashi, J. Brujic, M. So, and M. P. Sheetz (2010)
PNAS 107, 11358-11363
   Abstract »    Full Text »    PDF »
Reconstitution of a Functional Toll-like Receptor 5 Binding Site in Campylobacter jejuni Flagellin.
M. R. de Zoete, A. M. Keestra, J. A. Wagenaar, and J. P. M. van Putten (2010)
J. Biol. Chem. 285, 12149-12158
   Abstract »    Full Text »    PDF »
Functional Characterization of Flagellin Glycosylation in Campylobacter jejuni 81-176.
C. P. Ewing, E. Andreishcheva, and P. Guerry (2009)
J. Bacteriol. 191, 7086-7093
   Abstract »    Full Text »    PDF »
Effect of FliK mutation on the transcriptional activity of the {sigma}54 sigma factor RpoN in Helicobacter pylori.
F. P. Douillard, K. A. Ryan, J. Hinds, and P. W. O'Toole (2009)
Microbiology 155, 1901-1911
   Abstract »    Full Text »    PDF »
Posttranscriptional Regulation of Flagellin Synthesis in Helicobacter pylori by the RpoN Chaperone HP0958.
F. P. Douillard, K. A. Ryan, D. L. Caly, J. Hinds, A. A. Witney, S. E. Husain, and P. W. O'Toole (2008)
J. Bacteriol. 190, 7975-7984
   Abstract »    Full Text »    PDF »
Role of the Campylobacter jejuni Cj1461 DNA Methyltransferase in Regulating Virulence Characteristics.
J.-S. Kim, J. Li, I. H. A. Barnes, D. A. Baltzegar, M. Pajaniappan, T. W. Cullen, M. S. Trent, C. M. Burns, and S. A. Thompson (2008)
J. Bacteriol. 190, 6524-6529
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882