Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 320 (5881): 1349-1352

Copyright © 2008 by the American Association for the Advancement of Science

The Transcription/Migration Interface in Heart Precursors of Ciona intestinalis

Lionel Christiaen,1* Brad Davidson,1{dagger} Takeshi Kawashima,2 Weston Powell,1 Hector Nolla,3 Karen Vranizan,4 Michael Levine1*

Abstract: Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.

1 Department of Molecular and Cell Biology, Division of Genetics, Genomics and Development, Center for Integrative Genomics, University of California, Berkeley, CA 94720, USA.
2 Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA.
3 Cancer Research Laboratory, University of California, Berkeley, CA 94720, USA.
4 Functional Genomics Laboratory, University of California, Berkeley, CA 94720, USA.

{dagger} Present address: Department of Molecular and Cellular Biology, Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ 85724, USA.

* To whom correspondence should be addressed. E-mail: lionelchristiaen{at}berkeley.edu (L.C.); mlevine{at}berkeley.edu (M.L.)


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Heart genetics in a small package, exploiting the condensed genome of Ciona intestinalis.
C. D. Cota, F. Segade, and B. Davidson (2014)
Briefings in Functional Genomics 13, 3-14
   Abstract »    Full Text »    PDF »
Tbx2/3 is an essential mediator within the Brachyury gene network during Ciona notochord development.
D. S. Jose-Edwards, I. Oda-Ishii, Y. Nibu, and A. Di Gregorio (2013)
Development 140, 2422-2433
   Abstract »    Full Text »    PDF »
Matrix adhesion polarizes heart progenitor induction in the invertebrate chordate Ciona intestinalis.
J. Norton, J. Cooley, A. F. M. T. Islam, C. D. Cota, and B. Davidson (2013)
Development 140, 1301-1311
   Abstract »    Full Text »    PDF »
Functional transcriptomics of a migrating cell in Caenorhabditis elegans.
E. M. Schwarz, M. Kato, and P. W. Sternberg (2012)
PNAS 109, 16246-16251
   Abstract »    Full Text »    PDF »
Genetic and Genomic Toolbox of the Chordate Ciona intestinalis.
A. Stolfi and L. Christiaen (2012)
Genetics 192, 55-66
   Abstract »    Full Text »    PDF »
The Aplnr GPCR regulates myocardial progenitor development via a novel cell-non-autonomous, G{alpha}i/o protein-independent pathway.
S. Paskaradevan and I. C. Scott (2012)
Biology Open 1, 275-285
   Abstract »    Full Text »    PDF »
Smarcd3b and Gata5 promote a cardiac progenitor fate in the zebrafish embryo.
X. Lou, A. R. Deshwar, J. G. Crump, and I. C. Scott (2011)
Development 138, 3113-3123
   Abstract »    Full Text »    PDF »
Evolutionary crossroads in developmental biology: the tunicates.
P. Lemaire (2011)
Development 138, 2143-2152
   Abstract »    Full Text »    PDF »
FGF/MAPK/Ets signaling renders pigment cell precursors competent to respond to Wnt signal by directly controlling Ci-Tcf transcription.
P. Squarzoni, F. Parveen, L. Zanetti, F. Ristoratore, and A. Spagnuolo (2011)
Development 138, 1421-1432
   Abstract »    Full Text »    PDF »
Cardiopoietic Factors: Extracellular Signals for Cardiac Lineage Commitment.
M. Noseda, T. Peterkin, F. C. Simoes, R. Patient, and M. D. Schneider (2011)
Circ. Res. 108, 129-152
   Abstract »    Full Text »    PDF »
Mesp1: A Key Regulator of Cardiovascular Lineage Commitment.
A. Bondue and C. Blanpain (2010)
Circ. Res. 107, 1414-1427
   Abstract »    Full Text »    PDF »
The ANISEED database: Digital representation, formalization, and elucidation of a chordate developmental program.
O. Tassy, D. Dauga, F. Daian, D. Sobral, F. Robin, P. Khoueiry, D. Salgado, V. Fox, D. Caillol, R. Schiappa, et al. (2010)
Genome Res. 20, 1459-1468
   Abstract »    Full Text »    PDF »
Early Chordate Origins of the Vertebrate Second Heart Field.
A. Stolfi, T. B. Gainous, J. J. Young, A. Mori, M. Levine, and L. Christiaen (2010)
Science 329, 565-568
   Abstract »    Full Text »    PDF »
The FoxF/FoxC factor LET-381 directly regulates both cell fate specification and cell differentiation in C. elegans mesoderm development.
N. M. Amin, H. Shi, and J. Liu (2010)
Development 137, 1451-1460
   Abstract »    Full Text »    PDF »
Direct activation of Shroom3 transcription by Pitx proteins drives epithelial morphogenesis in the developing gut.
M.-I Chung, N. M. Nascone-Yoder, S. A. Grover, T. A. Drysdale, and J. B. Wallingford (2010)
Development 137, 1339-1349
   Abstract »    Full Text »    PDF »
An Integrated View of Precambrian Eumetazoan Evolution.
E. H. Davidson and D. H. Erwin (2010)
Cold Spring Harb Symp Quant Biol
   Abstract »    PDF »
The Sea Squirt Ciona intestinalis.
L. Christiaen, E. Wagner, W. Shi, and M. Levine (2009)
Cold Spring Harb Protoc 2009, pdb.emo138
   Abstract »    Full Text »    PDF »
Gene-regulatory networks in the Ciona embryos.
A. Kubo, K. S. Imai, and Y. Satou (2009)
Briefings in Functional Genomics 8, 250-255
   Abstract »    Full Text »    PDF »
Pushing the frontiers of development.
Y. Bellaiche and E. Munro (2009)
Development 136, 173-177
   Abstract »    Full Text »    PDF »
FGF3 in the floor plate directs notochord convergent extension in the Ciona tadpole.
W. Shi, S. M. Peyrot, E. Munro, and M. Levine (2009)
Development 136, 23-28
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882