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Science 320 (5883): 1651-1654

Copyright © 2008 by the American Association for the Advancement of Science

Ankyrin Repeat Proteins Comprise a Diverse Family of Bacterial Type IV Effectors

Xiaoxiao Pan,1 Anja Lührmann,1* Ayano Satoh,2* Michelle A. Laskowski-Arce,1{dagger} Craig R. Roy1{ddagger}

Abstract: Specialized secretion systems are used by many bacteria to deliver effector proteins into host cells that can either mimic or disrupt the function of eukaryotic factors. We found that the intracellular pathogens Legionella pneumophila and Coxiella burnetii use a type IV secretion system to deliver into eukaryotic cells a large number of different bacterial proteins containing ankyrin repeat homology domains called Anks. The L. pneumophila AnkX protein prevented microtubule-dependent vesicular transport to interfere with fusion of the L. pneumophila-containing vacuole with late endosomes after infection of macrophages, which demonstrates that Ank proteins have effector functions important for bacterial infection of eukaryotic host cells.

1 Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.
2 Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.

* These authors contributed equally to this work.

{dagger} Present address: University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, NA5.124, Dallas, TX 75390, USA.

{ddagger} To whom correspondence should be addressed. E-mail: craig.roy{at}yale.edu

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