Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 321 (5888): 557-560

Copyright © 2008 by the American Association for the Advancement of Science

BSKs Mediate Signal Transduction from the Receptor Kinase BRI1 in Arabidopsis

Wenqiang Tang,1 Tae-Wuk Kim,1 Juan A. Oses-Prieto,2 Yu Sun,1 Zhiping Deng,1 Shengwei Zhu,1,3 Ruiju Wang,1,4 Alma L. Burlingame,2 Zhi-Yong Wang1*

Abstract: Brassinosteroids (BRs) bind to the extracellular domain of the receptor kinase BRI1 to activate a signal transduction cascade that regulates nuclear gene expression and plant development. Many components of the BR signaling pathway have been identified and studied in detail. However, the substrate of BRI1 kinase that transduces the signal to downstream components remains unknown. Proteomic studies of plasma membrane proteins lead to the identification of three homologous BR-signaling kinases (BSK1, BSK2, and BSK3). The BSKs are phosphorylated by BRI1 in vitro and interact with BRI1 in vivo. Genetic and transgenic studies demonstrate that the BSKs represent a small family of kinases that activate BR signaling downstream of BRI1. These results demonstrate that BSKs are the substrates of BRI1 kinase that activate downstream BR signal transduction.

1 Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305, USA.
2 Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA.
3 Key Laboratory of Photosynthesis and Environmental Molecular Biology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
4 Institute for Molecular Biology, College of Life Science, Nankai University, Tianjin 300071, China.

* To whom correspondence should be addressed. E-mail: zywang24{at}stanford.edu

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis.
P. Liu, Z.-S. Xu, P.-P. Lu, D. Hu, M. Chen, L.-C. Li, and Y.-Z. Ma (2013)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Brassinosteroid biosynthesis and signalling in Petunia hybrida.
N. Verhoef, T. Yokota, K. Shibata, G.-J. de Boer, T. Gerats, M. Vandenbussche, R. Koes, and E. Souer (2013)
J. Exp. Bot. 64, 2435-2448
   Abstract »    Full Text »    PDF »
What Causes Opposing Actions of Brassinosteroids on Stomatal Development?.
L. Serna (2013)
Plant Physiology 162, 3-8
   Full Text »    PDF »
Brassinosteroid signalling.
J.-Y. Zhu, J. Sae-Seaw, and Z.-Y. Wang (2013)
Development 140, 1615-1620
   Abstract »    Full Text »    PDF »
BR Signal Influences Arabidopsis Ovule and Seed Number through Regulating Related Genes Expression by BZR1.
H.-Y. Huang, W.-B. Jiang, Y.-W. Hu, P. Wu, J.-Y. Zhu, W.-Q. Liang, Z.-Y. Wang, and W.-H. Lin (2013)
Mol Plant 6, 456-469
   Abstract »    Full Text »    PDF »
BR-SIGNALING KINASE1 Physically Associates with FLAGELLIN SENSING2 and Regulates Plant Innate Immunity in Arabidopsis.
H. Shi, Q. Shen, Y. Qi, H. Yan, H. Nie, Y. Chen, T. Zhao, F. Katagiri, and D. Tang (2013)
PLANT CELL 25, 1143-1157
   Abstract »    Full Text »    PDF »
Sensing the environment: key roles of membrane-localized kinases in plant perception and response to abiotic stress.
Y. Osakabe, K. Yamaguchi-Shinozaki, K. Shinozaki, and L.-S. P. Tran (2013)
J. Exp. Bot. 64, 445-458
   Abstract »    Full Text »    PDF »
MYBL2 is a substrate of GSK3-like kinase BIN2 and acts as a corepressor of BES1 in brassinosteroid signaling pathway in Arabidopsis.
H. Ye, L. Li, H. Guo, and Y. Yin (2012)
PNAS 109, 20142-20147
   Abstract »    Full Text »    PDF »
A Triple Helix-Loop-Helix/Basic Helix-Loop-Helix Cascade Controls Cell Elongation Downstream of Multiple Hormonal and Environmental Signaling Pathways in Arabidopsis.
M.-Y. Bai, M. Fan, E. Oh, and Z.-Y. Wang (2012)
PLANT CELL 24, 4917-4929
   Abstract »    Full Text »    PDF »
An Interaction Between BZR1 and DELLAs Mediates Direct Signaling Crosstalk Between Brassinosteroids and Gibberellins in Arabidopsis.
Q.-F. Li, C. Wang, L. Jiang, S. Li, S. S. M. Sun, and J.-X. He (2012)
Science Signaling 5, ra72
   Abstract »    Full Text »    PDF »
Arabidopsis MICROTUBULE DESTABILIZING PROTEIN40 Is Involved in Brassinosteroid Regulation of Hypocotyl Elongation.
X. Wang, J. Zhang, M. Yuan, D. W. Ehrhardt, Z. Wang, and T. Mao (2012)
PLANT CELL 24, 4012-4025
   Abstract »    Full Text »    PDF »
GSK3-Like Kinases Integrate Brassinosteroid Signaling and Stomatal Development.
S. A. Casson and A. M. Hetherington (2012)
Science Signaling 5, pe30
   Abstract »    Full Text »    PDF »
Brassinosteroid action in flowering plants: a Darwinian perspective.
U. Kutschera and Z.-Y. Wang (2012)
J. Exp. Bot. 63, 3511-3522
   Abstract »    Full Text »    PDF »
A Plasma Membrane Receptor Kinase, GHR1, Mediates Abscisic Acid- and Hydrogen Peroxide-Regulated Stomatal Movement in Arabidopsis.
D. Hua, C. Wang, J. He, H. Liao, Y. Duan, Z. Zhu, Y. Guo, Z. Chen, and Z. Gong (2012)
PLANT CELL 24, 2546-2561
   Abstract »    Full Text »    PDF »
DWARF AND LOW-TILLERING Acts as a Direct Downstream Target of a GSK3/SHAGGY-Like Kinase to Mediate Brassinosteroid Responses in Rice.
H. Tong, L. Liu, Y. Jin, L. Du, Y. Yin, Q. Qian, L. Zhu, and C. Chu (2012)
PLANT CELL 24, 2562-2577
   Abstract »    Full Text »    PDF »
Brassinosteroids Antagonize Gibberellin- and Salicylate-Mediated Root Immunity in Rice.
D. De Vleesschauwer, E. Van Buyten, K. Satoh, J. Balidion, R. Mauleon, I.-R. Choi, C. Vera-Cruz, S. Kikuchi, and M. Hofte (2012)
Plant Physiology 158, 1833-1846
   Abstract »    Full Text »    PDF »
Deactivation of the Arabidopsis BRASSINOSTEROID INSENSITIVE 1 (BRI1) receptor kinase by autophosphorylation within the glycine-rich loop.
M.-H. Oh, X. Wang, S. D. Clouse, and S. C. Huber (2012)
PNAS 109, 327-332
   Abstract »    Full Text »    PDF »
Quantification of the Brassinosteroid Insensitive1 Receptor in Planta.
G. Wilma van Esse, A. H. Westphal, R. P. Surendran, C. Albrecht, B. van Veen, J. W. Borst, and S. C. de Vries (2011)
Plant Physiology 156, 1691-1700
   Abstract »    Full Text »    PDF »
The Mechanisms of Brassinosteroids' Action: From Signal Transduction to Plant Development.
C.-J. Yang, C. Zhang, Y.-N. Lu, J.-Q. Jin, and X.-L. Wang (2011)
Mol Plant 4, 588-600
   Abstract »    Full Text »    PDF »
PP2A Phosphatases: The "On-Off" Regulatory Switches of Brassinosteroid Signaling.
S. Di Rubbo, N. G. Irani,, and E. Russinova (2011)
Science Signaling 4, pe25
   Abstract »    Full Text »    PDF »
Methylation of a Phosphatase Specifies Dephosphorylation and Degradation of Activated Brassinosteroid Receptors.
G. Wu, X. Wang, X. Li, Y. Kamiya, M. S. Otegui, and J. Chory (2011)
Science Signaling 4, ra29
   Abstract »    Full Text »    PDF »
Brassinosteroid Signal Transduction: From Receptor Kinase Activation to Transcriptional Networks Regulating Plant Development.
S. D. Clouse (2011)
PLANT CELL 23, 1219-1230
   Abstract »    Full Text »    PDF »
Proteomics Analysis Reveals Post-Translational Mechanisms for Cold-Induced Metabolic Changes in Arabidopsis.
T. Li, S.-L. Xu, J. A. Oses-Prieto, S. Putil, P. Xu, R.-J. Wang, K. H. Li, D. A. Maltby, L.-H. An, A. L. Burlingame, et al. (2011)
Mol Plant 4, 361-374
   Abstract »    Full Text »    PDF »
Taking the very first steps: from polarity to axial domains in the early Arabidopsis embryo.
S. Jeong, M. Bayer, and W. Lukowitz (2011)
J. Exp. Bot. 62, 1687-1697
   Abstract »    Full Text »    PDF »
MASCP Gator: An Aggregation Portal for the Visualization of Arabidopsis Proteomics Data.
H. J. Joshi, M. Hirsch-Hoffmann, K. Baerenfaller, W. Gruissem, S. Baginsky, R. Schmidt, W. X. Schulze, Q. Sun, K. J. van Wijk, V. Egelhofer, et al. (2011)
Plant Physiology 155, 259-270
   Abstract »    Full Text »    PDF »
Dramatic Change in Function and Expression Pattern of a Gene Duplicated by Polyploidy Created a Paternal Effect Gene in the Brassicaceae.
S.-L. Liu and K. L. Adams (2010)
Mol. Biol. Evol. 27, 2817-2828
   Abstract »    Full Text »    PDF »
Involvement of brassinosteroid signals in the floral-induction network of Arabidopsis.
J. Li, Y. Li, S. Chen, and L. An (2010)
J. Exp. Bot. 61, 4221-4230
   Abstract »    Full Text »    PDF »
Signaling from the Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes Acclimation to Stress in Arabidopsis.
P. Che, J. D. Bussell, W. Zhou, G. M. Estavillo, B. J. Pogson, and S. M. Smith (2010)
Science Signaling 3, ra69
   Abstract »    Full Text »    PDF »
Plant Immunity Triggered by Microbial Molecular Signatures.
J. Zhang and J.-M. Zhou (2010)
Mol Plant 3, 783-793
   Abstract »    Full Text »    PDF »
Intragenic Suppression of a Trafficking-Defective Brassinosteroid Receptor Mutant in Arabidopsis.
Y. Belkhadir, A. Durbak, M. Wierzba, R. J. Schmitz, A. Aguirre, R. Michel, S. Rowe, S. Fujioka, and F. E. Tax (2010)
Genetics 185, 1283-1296
   Abstract »    Full Text »    PDF »
Monitoring the Outside: Cell Wall-Sensing Mechanisms.
C. Ringli (2010)
Plant Physiology 153, 1445-1452
   Full Text »    PDF »
TCP1 Modulates Brassinosteroid Biosynthesis by Regulating the Expression of the Key Biosynthetic Gene DWARF4 in Arabidopsis thaliana.
Z. Guo, S. Fujioka, E. B. Blancaflor, S. Miao, X. Gou, and J. Li (2010)
PLANT CELL 22, 1161-1173
   Abstract »    Full Text »    PDF »
Arabidopsis IWS1 interacts with transcription factor BES1 and is involved in plant steroid hormone brassinosteroid regulated gene expression.
L. Li, H. Ye, H. Guo, and Y. Yin (2010)
PNAS 107, 3918-3923
   Abstract »    Full Text »    PDF »
A receptor-like cytoplasmic kinase, BIK1, associates with a flagellin receptor complex to initiate plant innate immunity.
D. Lu, S. Wu, X. Gao, Y. Zhang, L. Shan, and P. He (2010)
PNAS 107, 496-501
   Abstract »    Full Text »    PDF »
Activation-Tagged Suppressors of a Weak Brassinosteroid Receptor Mutant.
B. Kang, H. Wang, K. H. Nam, J. Li, and J. Li (2010)
Mol Plant 3, 260-268
   Abstract »    Full Text »    PDF »
Regulation of Arabidopsis Brassinosteroid Signaling by Atypical Basic Helix-Loop-Helix Proteins.
H. Wang, Y. Zhu, S. Fujioka, T. Asami, J. Li, and J. Li (2009)
PLANT CELL 21, 3781-3791
   Abstract »    Full Text »    PDF »
Antagonistic HLH/bHLH Transcription Factors Mediate Brassinosteroid Regulation of Cell Elongation and Plant Development in Rice and Arabidopsis.
L.-Y. Zhang, M.-Y. Bai, J. Wu, J.-Y. Zhu, H. Wang, Z. Zhang, W. Wang, Y. Sun, J. Zhao, X. Sun, et al. (2009)
PLANT CELL 21, 3767-3780
   Abstract »    Full Text »    PDF »
A Leaky Mutation in DWARF4 Reveals an Antagonistic Role of Brassinosteroid in the Inhibition of Root Growth by Jasmonate in Arabidopsis.
C. Ren, C. Han, W. Peng, Y. Huang, Z. Peng, X. Xiong, Q. Zhu, B. Gao, and D. Xie (2009)
Plant Physiology 151, 1412-1420
   Abstract »    Full Text »    PDF »
BRASSINOSTEROID UPREGULATED1, Encoding a Helix-Loop-Helix Protein, Is a Novel Gene Involved in Brassinosteroid Signaling and Controls Bending of the Lamina Joint in Rice.
A. Tanaka, H. Nakagawa, C. Tomita, Z. Shimatani, M. Ohtake, T. Nomura, C.-J. Jiang, J. G. Dubouzet, S. Kikuchi, H. Sekimoto, et al. (2009)
Plant Physiology 151, 669-680
   Abstract »    Full Text »    PDF »
BIN2 Functions Redundantly with Other Arabidopsis GSK3-Like Kinases to Regulate Brassinosteroid Signaling.
Z. Yan, J. Zhao, P. Peng, R. K. Chihara, and J. Li (2009)
Plant Physiology 150, 710-721
   Abstract »    Full Text »    PDF »
Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana.
H. Guo, L. Li, H. Ye, X. Yu, A. Algreen, and Y. Yin (2009)
PNAS 106, 7648-7653
   Abstract »    Full Text »    PDF »
The primary signaling outputs of brassinosteroids are regulated by abscisic acid signaling.
S. Zhang, Z. Cai, and X. Wang (2009)
PNAS 106, 4543-4548
   Abstract »    Full Text »    PDF »
Function of the {alpha} Subunit of Rice Heterotrimeric G Protein in Brassinosteroid Signaling.
K. Oki, N. Inaba, K. Kitagawa, S. Fujioka, H. Kitano, Y. Fujisawa, H. Kato, and Y. Iwasaki (2009)
Plant Cell Physiol. 50, 161-172
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882