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Molecular Architecture of the "Stressosome," a Signal Integration and Transduction Hub
Gijs van Duinen,2
Susan J. Firbank,1
Peter J. Lewis,3
James W. Murray,1
Joseph A. Newman,1
Maureen B. Quin,1
Paul R. Race,1
Marin van Heel,2¶
Richard J. Lewis1¶
A commonly used strategy by microorganisms to survive multiplestresses involves a signal transduction cascade that increasesthe expression of stress-responsive genes. Stress signals canbe integrated by a multiprotein signaling hub that respondsto various signals to effect a single outcome. We obtained amedium-resolution cryo–electron microscopy reconstructionof the 1.8-megadalton "stressosome" from Bacillus subtilis.Fitting known crystal structures of components into this reconstructiongave a pseudoatomic structure, which had a virus capsid–likecore with sensory extensions. We suggest that the differentsensory extensions respond to different signals, whereas theconserved domains in the core integrate the varied signals.The architecture of the stressosome provides the potential forcooperativity, suggesting that the response could be tuned dependenton the magnitude of chemophysical insult.
1 Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle-upon-Tyne NE2 4HH, UK. 2 Faculty of Natural Sciences, Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK. 3 School of Environmental and Life Sciences, University of Newcastle, Collaghan, Newcastle, NSW 2308, Australia.
* These authors contributed equally to this work.
Present address: Laboratoire de Génétique Microbienne,Domaine de Vilvert, Institut de la Recherche Agronomique INRA,78350 Jouy-en-Josas, France.