Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 322 (5901): 590-594

Copyright © 2008 by the American Association for the Advancement of Science

TMEM16A, A Membrane Protein Associated with Calcium-Dependent Chloride Channel Activity

Antonella Caputo,1 Emanuela Caci,1 Loretta Ferrera,1 Nicoletta Pedemonte,2 Cristina Barsanti,1 Elvira Sondo,1 Ulrich Pfeffer,3 Roberto Ravazzolo,1 Olga Zegarra-Moran,1 Luis J. V. Galietta1,2*

Abstract: Calcium-dependent chloride channels are required for normal electrolyte and fluid secretion, olfactory perception, and neuronal and smooth muscle excitability. The molecular identity of these membrane proteins is still unclear. Treatment of bronchial epithelial cells with interleukin-4 (IL-4) causes increased calcium-dependent chloride channel activity, presumably by regulating expression of the corresponding genes. We performed a global gene expression analysis to identify membrane proteins that are regulated by IL-4. Transfection of epithelial cells with specific small interfering RNA against each of these proteins shows that TMEM16A, a member of a family of putative plasma membrane proteins with unknown function, is associated with calcium-dependent chloride current, as measured with halide-sensitive fluorescent proteins, short-circuit current, and patch-clamp techniques. Our results indicate that TMEM16A is an intrinsic constituent of the calcium-dependent chloride channel. Identification of a previously unknown family of membrane proteins associated with chloride channel function will improve our understanding of chloride transport physiopathology and allow for the development of pharmacological tools useful for basic research and drug development.

1 Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova 16148, Italy.
2 Centro di Biotecnologie Avanzate, Genova 16132, Italy.
3 National Cancer Research Institute, Genova 16132, Italy.

* To whom correspondence should be addressed. E-mail: galietta{at}unige.it


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Small Molecule-facilitated Degradation of ANO1 Protein: A NEW TARGETING APPROACH FOR ANTICANCER THERAPEUTICS.
A. Bill, M. L. Hall, J. Borawski, C. Hodgson, J. Jenkins, P. Piechon, O. Popa, C. Rothwell, P. Tranter, S. Tria, et al. (2014)
J. Biol. Chem. 289, 11029-11041
   Abstract »    Full Text »    PDF »
Computational modeling of anoctamin 1 calcium-activated chloride channels as pacemaker channels in interstitial cells of Cajal.
R. Lees-Green, S. J. Gibbons, G. Farrugia, J. Sneyd, and L. K. Cheng (2014)
Am J Physiol Gastrointest Liver Physiol 306, G711-G727
   Abstract »    Full Text »    PDF »
Structure and Function of TMEM16 Proteins (Anoctamins).
N. Pedemonte and L. J. V. Galietta (2014)
Physiol Rev 94, 419-459
   Abstract »    Full Text »    PDF »
Glucose stimulates calcium-activated chloride secretion in small intestinal cells.
L. Yin, P. Vijaygopal, G. G. MacGregor, R. Menon, P. Ranganathan, S. Prabhakaran, L. Zhang, M. Zhang, H. J. Binder, P. Okunieff, et al. (2014)
Am J Physiol Cell Physiol 306, C687-C696
   Abstract »    Full Text »    PDF »
Expression and function of a T-type Ca2+ conductance in interstitial cells of Cajal of the murine small intestine.
H. Zheng, K. S. Park, S. D. Koh, and K. M. Sanders (2014)
Am J Physiol Cell Physiol 306, C705-C713
   Abstract »    Full Text »    PDF »
Role of anoctamins in cancer and apoptosis.
P. Wanitchakool, L. Wolf, G. E. Koehl, L. Sirianant, R. Schreiber, S. Kulkarni, U. Duvvuri, and K. Kunzelmann (2014)
Phil Trans R Soc B 369, 20130096
   Abstract »    Full Text »    PDF »
Functional Swapping between Transmembrane Proteins TMEM16A and TMEM16F.
T. Suzuki, J. Suzuki, and S. Nagata (2014)
J. Biol. Chem. 289, 7438-7447
   Abstract »    Full Text »    PDF »
Kv7 and Kv11 channels in myometrial regulation.
I. A. Greenwood and R. M. Tribe (2014)
Exp Physiol 99, 503-509
   Abstract »    Full Text »    PDF »
Activation of the Ano1 (TMEM16A) chloride channel by calcium is not mediated by calmodulin.
K. Yu, J. Zhu, Z. Qu, Y.-Y. Cui, and H. C. Hartzell (2014)
J. Gen. Physiol. 143, 253-267
   Abstract »    Full Text »    PDF »
Phylogenetic, expression, and functional analyses of anoctamin homologs in Caenorhabditis elegans.
Y. Wang, T. Alam, K. Hill-Harfe, A. J. Lopez, C. K. Leung, D. Iribarne, B. Bruggeman, M. M. Miyamoto, B. D. Harfe, and K. P. Choe (2013)
Am J Physiol Regulatory Integrative Comp Physiol 305, R1376-R1389
   Abstract »    Full Text »    PDF »
Functional reconstitution of a chloride channel bares its soul.
H. C. Hartzell and C. C. Ruppersburg (2013)
PNAS 110, 19185-19186
   Full Text »    PDF »
Purified TMEM16A is sufficient to form Ca2+-activated Cl- channels.
H. Terashima, A. Picollo, and A. Accardi (2013)
PNAS 110, 19354-19359
   Abstract »    Full Text »    PDF »
Subdued, a TMEM16 family Ca2+-activated Cl- channel in Drosophila melanogaster with an unexpected role in host defense.
X. M. Wong, S. Younger, C. J. Peters, Y. N. Jan, and L. Y. Jan (2013)
eLife Sci 2, e00862
   Abstract »    Full Text »    PDF »
MONNA, a Potent and Selective Blocker for Transmembrane Protein with Unknown Function 16/Anoctamin-1.
S.-J. Oh, S. J. Hwang, J. Jung, K. Yu, J. Kim, J. Y. Choi, H. C. Hartzell, E. J. Roh, and C. J. Lee (2013)
Mol. Pharmacol. 84, 726-735
   Abstract »    Full Text »    PDF »
Functional expression of the TMEM16 family of calcium-activated chloride channels in airway smooth muscle.
G. Gallos, K. E. Remy, J. Danielsson, H. Funayama, X. W. Fu, H.-Y. S. Chang, P. Yim, D. Xu, and C. W. Emala Sr (2013)
Am J Physiol Lung Cell Mol Physiol 305, L625-L634
   Abstract »    Full Text »    PDF »
Cigarette smoke and CFTR: implications in the pathogenesis of COPD.
A. Rab, S. M. Rowe, S. V. Raju, Z. Bebok, S. Matalon, and J. F. Collawn (2013)
Am J Physiol Lung Cell Mol Physiol 305, L530-L541
   Abstract »    Full Text »    PDF »
Calmodulin-dependent activation and inactivation of anoctamin calcium-gated chloride channels.
K. Vocke, K. Dauner, A. Hahn, A. Ulbrich, J. Broecker, S. Keller, S. Frings, and F. Mohrlen (2013)
J. Gen. Physiol. 142, 381-404
   Abstract »    Full Text »    PDF »
Activation of the Cl- Channel ANO1 by Localized Calcium Signals in Nociceptive Sensory Neurons Requires Coupling with the IP3 Receptor.
X. Jin, S. Shah, Y. Liu, H. Zhang, M. Lees, Z. Fu, J. D. Lippiat, D. J. Beech, A. Sivaprasadarao, S. A. Baldwin, et al. (2013)
Science Signaling 6, ra73
   Abstract »    Full Text »    PDF »
Pore directions for the expression of a Ca2+-activated chloride channel.
B. D. Schultz (2013)
J. Physiol. 591, 3453-3454
   Full Text »    PDF »
Putative pore-loops of TMEM16/anoctamin channels affect channel density in cell membranes.
A. Adomaviciene, K. J. Smith, H. Garnett, and P. Tammaro (2013)
J. Physiol. 591, 3487-3505
   Abstract »    Full Text »    PDF »
The genetics of dystonia: new twists in an old tale.
G. Charlesworth, K. P. Bhatia, and N. W. Wood (2013)
Brain 136, 2017-2037
   Abstract »    Full Text »    PDF »
DOG1 Regulates Growth and IGFBP5 in Gastrointestinal Stromal Tumors.
S. Simon, F. Grabellus, L. Ferrera, L. Galietta, B. Schwindenhammer, T. Muhlenberg, G. Taeger, G. Eilers, J. Treckmann, F. Breitenbuecher, et al. (2013)
Cancer Res. 73, 3661-3670
   Abstract »    Full Text »    PDF »
Channel properties of the splicing isoforms of the olfactory calcium-activated chloride channel Anoctamin 2.
S. Ponissery Saidu, A. B. Stephan, A. K. Talaga, H. Zhao, and J. Reisert (2013)
J. Gen. Physiol. 141, 691-703
   Abstract »    Full Text »    PDF »
New Pulmonary Therapies Directed at Targets Other than CFTR.
S. H. Donaldson and L. Galietta (2013)
Cold Spring Harb Perspect Med 3, a009787
   Abstract »    Full Text »    PDF »
Calcium-dependent Phospholipid Scramblase Activity of TMEM16 Protein Family Members.
J. Suzuki, T. Fujii, T. Imao, K. Ishihara, H. Kuba, and S. Nagata (2013)
J. Biol. Chem. 288, 13305-13316
   Abstract »    Full Text »    PDF »
Targeted Expression of Anoctamin Calcium-Activated Chloride Channels in Rod Photoreceptor Terminals of the Rodent Retina.
K. Dauner, C. Mobus, S. Frings, and F. Mohrlen (2013)
Invest. Ophthalmol. Vis. Sci. 54, 3126-3136
   Abstract »    Full Text »    PDF »
The Cystic Fibrosis of Exocrine Pancreas.
M. Wilschanski and I. Novak (2013)
Cold Spring Harb Perspect Med 3, a009746
   Abstract »    Full Text »    PDF »
TMEM16F (Anoctamin 6), an anion channel of delayed Ca2+ activation.
S. Grubb, K. A. Poulsen, C. A. Juul, T. Kyed, T. K. Klausen, E. H. Larsen, and E. K. Hoffmann (2013)
J. Gen. Physiol. 141, 585-600
   Abstract »    Full Text »    PDF »
TMEM16F is a component of a Ca2+-activated Cl- channel but not a volume-sensitive outwardly rectifying Cl- channel.
T. Shimizu, T. Iehara, K. Sato, T. Fujii, H. Sakai, and Y. Okada (2013)
Am J Physiol Cell Physiol 304, C748-C759
   Abstract »    Full Text »    PDF »
Purinergic regulation of CFTR and Ca2+-activated Cl- channels and K+ channels in human pancreatic duct epithelium.
J. Wang, K. A. Haanes, and I. Novak (2013)
Am J Physiol Cell Physiol 304, C673-C684
   Abstract »    Full Text »    PDF »
Calcium-activated chloride channel ANO1 promotes breast cancer progression by activating EGFR and CAMK signaling.
A. Britschgi, A. Bill, H. Brinkhaus, C. Rothwell, I. Clay, S. Duss, M. Rebhan, P. Raman, C. T. Guy, K. Wetzel, et al. (2013)
PNAS 110, E1026-E1034
   Abstract »    Full Text »    PDF »
Dynamic modulation of ANO1/TMEM16A HCO3- permeability by Ca2+/calmodulin.
J. Jung, J. H. Nam, H. W. Park, U. Oh, J.-H. Yoon, and M. G. Lee (2013)
PNAS 110, 360-365
   Abstract »    Full Text »    PDF »
Mechanosensitive Cl- secretion in biliary epithelium mediated through TMEM16A.
A. K. Dutta, K. Woo, A.-k. Khimji, C. Kresge, and A. P. Feranchak (2013)
Am J Physiol Gastrointest Liver Physiol 304, G87-G98
   Abstract »    Full Text »    PDF »
Functional Regulation of ClC-3 in the Migration of Vascular Smooth Muscle Cells.
S. B. Ganapathi, S.-G. Wei, A. Zaremba, F. S. Lamb, and S. B. Shears (2013)
Hypertension 61, 174-179
   Abstract »    Full Text »    PDF »
Increased TMEM16A-encoded calcium-activated chloride channel activity is associated with pulmonary hypertension.
A. S. Forrest, T. C. Joyce, M. L. Huebner, R. J. Ayon, M. Wiwchar, J. Joyce, N. Freitas, A. J. Davis, L. Ye, D. D. Duan, et al. (2012)
Am J Physiol Cell Physiol 303, C1229-C1243
   Abstract »    Full Text »    PDF »
Association of TMEM16A chloride channel overexpression with airway goblet cell metaplasia.
P. Scudieri, E. Caci, S. Bruno, L. Ferrera, M. Schiavon, E. Sondo, V. Tomati, A. Gianotti, O. Zegarra-Moran, N. Pedemonte, et al. (2012)
J. Physiol. 590, 6141-6155
   Abstract »    Full Text »    PDF »
Anoctamins are a family of Ca2+-activated Cl- channels.
Y. Tian, R. Schreiber, and K. Kunzelmann (2012)
J. Cell Sci. 125, 4991-4998
   Abstract »    Full Text »    PDF »
Rescue of epithelial HCO3- secretion in murine intestine by apical membrane expression of the cystic fibrosis transmembrane conductance regulator mutant F508del.
F. Xiao, J. Li, A. K. Singh, B. Riederer, J. Wang, A. Sultan, H. Park, M. G. Lee, G. Lamprecht, B. J. Scholte, et al. (2012)
J. Physiol. 590, 5317-5334
   Abstract »    Full Text »    PDF »
Proinflammatory cytokine secretion is suppressed by TMEM16A or CFTR channel activity in human cystic fibrosis bronchial epithelia.
G. Veit, F. Bossard, J. Goepp, A. S. Verkman, L. J. V. Galietta, J. W. Hanrahan, and G. L. Lukacs (2012)
Mol. Biol. Cell 23, 4188-4202
   Abstract »    Full Text »    PDF »
Calcium-activated chloride channel TMEM16A modulates mucin secretion and airway smooth muscle contraction.
F. Huang, H. Zhang, M. Wu, H. Yang, M. Kudo, C. J. Peters, P. G. Woodruff, O. D. Solberg, M. L. Donne, X. Huang, et al. (2012)
PNAS 109, 16354-16359
   Abstract »    Full Text »    PDF »
Regional differences in rat conjunctival ion transport activities.
D. Yu, W. R. Thelin, T. D. Rogers, M. J. Stutts, S. H. Randell, B. R. Grubb, and R. C. Boucher (2012)
Am J Physiol Cell Physiol 303, C767-C780
   Abstract »    Full Text »    PDF »
Transepithelial Bicarbonate Secretion: Lessons from the Pancreas.
H. W. Park and M. G. Lee (2012)
Cold Spring Harb Perspect Med 2, a009571
   Abstract »    Full Text »    PDF »
TMEM16A/ANO1 Channels Contribute to the Myogenic Response in Cerebral Arteries.
S. Bulley, Z. P. Neeb, S. K. Burris, J. P. Bannister, C. M. Thomas-Gatewood, W. Jangsangthong, and J. H. Jaggar (2012)
Circ. Res. 111, 1027-1036
   Abstract »    Full Text »    PDF »
Differential effects of extracellular ATP on chloride transport in cortical collecting duct cells.
M. Rajagopal, P. P. Kathpalia, J. H. Widdicombe, and A. C. Pao (2012)
Am J Physiol Renal Physiol 303, F483-F491
   Abstract »    Full Text »    PDF »
Chronic hypoxia-induced upregulation of Ca2+-activated Cl- channel in pulmonary arterial myocytes: a mechanism contributing to enhanced vasoreactivity.
H. Sun, Y. Xia, O. Paudel, X.-R. Yang, and J. S. K. Sham (2012)
J. Physiol. 590, 3507-3521
   Abstract »    Full Text »    PDF »
TMEM16A Induces MAPK and Contributes Directly to Tumorigenesis and Cancer Progression.
U. Duvvuri, D. J. Shiwarski, D. Xiao, C. Bertrand, X. Huang, R. S. Edinger, J. R. Rock, B. D. Harfe, B. J. Henson, K. Kunzelmann, et al. (2012)
Cancer Res. 72, 3270-3281
   Abstract »    Full Text »    PDF »
Anoctamin 1 (Tmem16A) Ca2+-activated chloride channel stoichiometrically interacts with an ezrin-radixin-moesin network.
P. Perez-Cornejo, A. Gokhale, C. Duran, Y. Cui, Q. Xiao, H. C. Hartzell, and V. Faundez (2012)
PNAS 109, 10376-10381
   Abstract »    Full Text »    PDF »
Calcium-activated chloride channels in the apical region of mouse vomeronasal sensory neurons.
M. Dibattista, A. Amjad, D. K. Maurya, C. Sagheddu, G. Montani, R. Tirindelli, and A. Menini (2012)
J. Gen. Physiol. 140, 3-15
   Abstract »    Full Text »    PDF »
Human spermatozoa possess a calcium-dependent chloride channel that may participate in the acrosomal reaction.
G. Orta, G. Ferreira, O. Jose, C. L. Trevino, C. Beltran, and A. Darszon (2012)
J. Physiol. 590, 2659-2675
   Abstract »    Full Text »    PDF »
Physiology of Epithelial Chloride and Fluid Secretion.
R. A. Frizzell and J. W. Hanrahan (2012)
Cold Spring Harb Perspect Med 2, a009563
   Abstract »    Full Text »    PDF »
Epidermal growth factor chronically upregulates Ca2+-dependent Cl- conductance and TMEM16A expression in intestinal epithelial cells.
M. S. Mroz and S. J. Keely (2012)
J. Physiol. 590, 1907-1920
   Abstract »    Full Text »    PDF »
The voltage dependence of the TMEM16B/anoctamin2 calcium-activated chloride channel is modified by mutations in the first putative intracellular loop.
V. Cenedese, G. Betto, F. Celsi, O. L. Cherian, S. Pifferi, and A. Menini (2012)
J. Gen. Physiol. 139, 285-294
   Abstract »    Full Text »    PDF »
Explaining Calcium-Dependent Gating of Anoctamin-1 Chloride Channels Requires a Revised Topology.
K. Yu, C. Duran, Z. Qu, Y.-Y. Cui, and H. C. Hartzell (2012)
Circ. Res. 110, 990-999
   Abstract »    Full Text »    PDF »
Ca2+-activated Cl- channels at a glance.
J. Berg, H. Yang, and L. Y. Jan (2012)
J. Cell Sci. 125, 1367-1371
   Full Text »    PDF »
Downregulation of TMEM16A Calcium-Activated Chloride Channel Contributes to Cerebrovascular Remodeling During Hypertension by Promoting Basilar Smooth Muscle Cell Proliferation.
M. Wang, H. Yang, L.-Y. Zheng, Z. Zhang, Y.-B. Tang, G.-L. Wang, Y.-H. Du, X.-F. Lv, J. Liu, J.-G. Zhou, et al. (2012)
Circulation 125, 697-707
   Abstract »    Full Text »    PDF »
The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age.
J. P. Winpenny and M. A. Gray (2012)
Exp Physiol 97, 175-176
   Full Text »    PDF »
ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins.
C. Duran, Z. Qu, A. O. Osunkoya, Y. Cui, and H. C. Hartzell (2012)
Am J Physiol Cell Physiol 302, C482-C493
   Abstract »    Full Text »    PDF »
The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels.
P. Scudieri, E. Sondo, L. Ferrera, and L. J. V. Galietta (2012)
Exp Physiol 97, 177-183
   Abstract »    Full Text »    PDF »
Presence of the Ca2+-activated chloride channel anoctamin 1 in the urethra and its role in excitatory neurotransmission.
M. Sancho, A. Garcia-Pascual, and D. Triguero (2012)
Am J Physiol Renal Physiol 302, F390-F400
   Abstract »    Full Text »    PDF »
Expression and function of epithelial anoctamins.
K. Kunzelmann, R. Schreiber, A. Kmit, W. Jantarajit, J. R. Martins, D. Faria, P. Kongsuphol, J. Ousingsawat, and Y. Tian (2012)
Exp Physiol 97, 184-192
   Abstract »    Full Text »    PDF »
Anoctamin 2/TMEM16B: a calcium-activated chloride channel in olfactory transduction.
S. Pifferi, V. Cenedese, and A. Menini (2012)
Exp Physiol 97, 193-199
   Abstract »    Full Text »    PDF »
Anoctamins and gastrointestinal smooth muscle excitability.
K. M. Sanders, M. H. Zhu, F. Britton, S. D. Koh, and S. M. Ward (2012)
Exp Physiol 97, 200-206
   Abstract »    Full Text »    PDF »
Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCOFormula Secretion.
M. G. Lee, E. Ohana, H. W. Park, D. Yang, and S. Muallem (2012)
Physiol Rev 92, 39-74
   Abstract »    Full Text »    PDF »
Targeting ion channels for the treatment of gastrointestinal motility disorders.
A. Beyder and G. Farrugia (2012)
Therapeutic Advances in Gastroenterology 5, 5-21
   Abstract »    PDF »
Hypoxic Pulmonary Vasoconstriction.
J. T. Sylvester, L. A. Shimoda, P. I. Aaronson, and J. P. T. Ward (2012)
Physiol Rev 92, 367-520
   Abstract »    Full Text »    PDF »
International Union of Basic and Clinical Pharmacology. LXXXV: Calcium-Activated Chloride Channels.
F. Huang, X. Wong, and L. Y. Jan (2012)
Pharmacol. Rev. 64, 1-15
   Abstract »    Full Text »    PDF »
Divergent CFTR orthologs respond differently to the channel inhibitors CFTRinh-172, glibenclamide, and GlyH-101.
M. Stahl, K. Stahl, M. B. Brubacher, and J. N. Forrest Jr. (2012)
Am J Physiol Cell Physiol 302, C67-C76
   Abstract »    Full Text »    PDF »
Electrical Slow Waves in the Mouse Oviduct Are Dependent upon a Calcium Activated Chloride Conductance Encoded by Tmem16a.
R. E. Dixon, G. W. Hennig, S. A. Baker, F. C. Britton, B. D. Harfe, J. R. Rock, K. M. Sanders, and S. M. Ward (2012)
Biol Reprod 86, 1-7
   Abstract »    Full Text »    PDF »
Ano1 as a regulator of proliferation.
J. E. Stanich, S. J. Gibbons, S. T. Eisenman, M. R. Bardsley, J. R. Rock, B. D. Harfe, T. Ordog, and G. Farrugia (2011)
Am J Physiol Gastrointest Liver Physiol 301, G1044-G1051
   Abstract »    Full Text »    PDF »
Electrical slow waves in the mouse oviduct are dependent on extracellular and intracellular calcium sources.
R. E. Dixon, F. C. Britton, S. A. Baker, G. W. Hennig, C. M. Rollings, K. M. Sanders, and S. M. Ward (2011)
Am J Physiol Cell Physiol 301, C1458-C1469
   Abstract »    Full Text »    PDF »
Small-molecule activators of TMEM16A, a calcium-activated chloride channel, stimulate epithelial chloride secretion and intestinal contraction.
W. Namkung, Z. Yao, W. E. Finkbeiner, and A. S. Verkman (2011)
FASEB J 25, 4048-4062
   Abstract »    Full Text »    PDF »
Anoctamin 6 is an essential component of the outwardly rectifying chloride channel.
J. R. Martins, D. Faria, P. Kongsuphol, B. Reisch, R. Schreiber, and K. Kunzelmann (2011)
PNAS 108, 18168-18172
   Abstract »    Full Text »    PDF »
TMEM16A channels generate Ca2+-activated Cl- currents in cerebral artery smooth muscle cells.
C. Thomas-Gatewood, Z. P. Neeb, S. Bulley, A. Adebiyi, J. P. Bannister, M. D. Leo, and J. H. Jaggar (2011)
Am J Physiol Heart Circ Physiol 301, H1819-H1827
   Abstract »    Full Text »    PDF »
Comprehensive exon array data processing method for quantitative analysis of alternative spliced variants.
P. Chen, T. Lepikhova, Y. Hu, O. Monni, and S. Hautaniemi (2011)
Nucleic Acids Res. 39, e123
   Abstract »    Full Text »    PDF »
Muscarinic activation of Ca2+-activated Cl- current in interstitial cells of Cajal.
M. H. Zhu, I. K. Sung, H. Zheng, T. S. Sung, F. C. Britton, K. O'Driscoll, S. D. Koh, and K. M. Sanders (2011)
J. Physiol. 589, 4565-4582
   Abstract »    Full Text »    PDF »
Activation of P2Y1 and P2Y2 receptors induces chloride secretion via calcium-activated chloride channels in kidney inner medullary collecting duct cells.
M. Rajagopal, P. P. Kathpalia, S. V. Thomas, and A. C. Pao (2011)
Am J Physiol Renal Physiol 301, F544-F553
   Abstract »    Full Text »    PDF »
Rho Signaling Regulates Pannexin 1-mediated ATP Release from Airway Epithelia.
L. Seminario-Vidal, S. F. Okada, J. I. Sesma, S. M. Kreda, C. A. van Heusden, Y. Zhu, L. C. Jones, W. K. O'Neal, S. Penuela, D. W. Laird, et al. (2011)
J. Biol. Chem. 286, 26277-26286
   Abstract »    Full Text »    PDF »
Functional Apical Large Conductance, Ca2+-activated, and Voltage-dependent K+ Channels Are Required for Maintenance of Airway Surface Liquid Volume.
D. Manzanares, C. Gonzalez, P. Ivonnet, R.-S. Chen, M. Valencia-Gattas, G. E. Conner, H. P. Larsson, and M. Salathe (2011)
J. Biol. Chem. 286, 19830-19839
   Abstract »    Full Text »    PDF »
The distribution and expression of CFTR restricts electrogenic anion secretion to the ileum of the brushtail possum, Trichosurus vulpecula.
M. Gill, R. C. Bartolo, K. Demmers, N. Harfoot, S. Fan, and G. Butt (2011)
J. Exp. Biol. 214, 1943-1954
   Abstract »    Full Text »    PDF »
(n-3) Long-Chain PUFA Differentially Affect Resistance to Pseudomonas aeruginosa Infection of Male and Female cftr-/- Mice.
H. Tiesset, H. Bernard, N. Bartke, C. Beermann, E. Flachaire, J.-L. Desseyn, F. Gottrand, and M.-O. Husson (2011)
J. Nutr. 141, 1101-1107
   Abstract »    Full Text »    PDF »
Voltage- and calcium-dependent gating of TMEM16A/Ano1 chloride channels are physically coupled by the first intracellular loop.
Q. Xiao, K. Yu, P. Perez-Cornejo, Y. Cui, J. Arreola, and H. C. Hartzell (2011)
PNAS 108, 8891-8896
   Abstract »    Full Text »    PDF »
Altered Expression of Ano1 Variants in Human Diabetic Gastroparesis.
A. Mazzone, C. E. Bernard, P. R. Strege, A. Beyder, L. J. V. Galietta, P. J. Pasricha, J. L. Rae, H. P. Parkman, D. R. Linden, J. H. Szurszewski, et al. (2011)
J. Biol. Chem. 286, 13393-13403
   Abstract »    Full Text »    PDF »
Phospholipase C-{beta}3 Is a Key Modulator of IL-8 Expression in Cystic Fibrosis Bronchial Epithelial Cells.
V. Bezzerri, P. d'Adamo, A. Rimessi, C. Lanzara, S. Crovella, E. Nicolis, A. Tamanini, E. Athanasakis, M. Tebon, G. Bisoffi, et al. (2011)
J. Immunol. 186, 4946-4958
   Abstract »    Full Text »    PDF »
The {Delta}F508 Mutation Causes CFTR Misprocessing and Cystic Fibrosis-Like Disease in Pigs.
L. S. Ostedgaard, D. K. Meyerholz, J.-H. Chen, A. A. Pezzulo, P. H. Karp, T. Rokhlina, S. E. Ernst, R. A. Hanfland, L. R. Reznikov, P. S. Ludwig, et al. (2011)
Science Translational Medicine 3, 74ra24
   Abstract »    Full Text »    PDF »
Calmodulin-dependent activation of the epithelial calcium-dependent chloride channel TMEM16A.
Y. Tian, P. Kongsuphol, M. Hug, J. Ousingsawat, R. Witzgall, R. Schreiber, and K. Kunzelmann (2011)
FASEB J 25, 1058-1068
   Abstract »    Full Text »    PDF »
Activation of the basolateral membrane Cl- conductance essential for electrogenic K+ secretion suppresses electrogenic Cl- secretion.
Q. He, S. T. Halm, J. Zhang, and D. R. Halm (2011)
Exp Physiol 96, 305-316
   Abstract »    Full Text »    PDF »
TMEM16A(a)/anoctamin-1 Shares a Homodimeric Architecture with CLC Chloride Channels.
G. Fallah, T. Romer, S. Detro-Dassen, U. Braam, F. Markwardt, and G. Schmalzing (2011)
Mol. Cell. Proteomics 10, M110.004697
   Abstract »    Full Text »    PDF »
TMEM16A Inhibitors Reveal TMEM16A as a Minor Component of Calcium-activated Chloride Channel Conductance in Airway and Intestinal Epithelial Cells.
W. Namkung, P.-W. Phuan, and A. S. Verkman (2011)
J. Biol. Chem. 286, 2365-2374
   Abstract »    Full Text »    PDF »
Characterization of the Oligomeric Structure of the Ca2+-activated Cl- Channel Ano1/TMEM16A.
J. T. Sheridan, E. N. Worthington, K. Yu, S. E. Gabriel, H. C. Hartzell, and R. Tarran (2011)
J. Biol. Chem. 286, 1381-1388
   Abstract »    Full Text »    PDF »
Identification and Functional Characterization of TMEM16A, a Ca2+-activated Cl- Channel Activated by Extracellular Nucleotides, in Biliary Epithelium.
A. K. Dutta, A.-k. Khimji, C. Kresge, A. Bugde, M. Dougherty, V. Esser, Y. Ueno, S. S. Glaser, G. Alpini, D. C. Rockey, et al. (2011)
J. Biol. Chem. 286, 766-776
   Abstract »    Full Text »    PDF »
Location of Release Sites and Calcium-Activated Chloride Channels Relative to Calcium Channels at the Photoreceptor Ribbon Synapse.
A. J. Mercer, K. Rabl, G. E. Riccardi, N. C. Brecha, S. L. Stella Jr, and W. B. Thoreson (2011)
J Neurophysiol 105, 321-335
   Abstract »    Full Text »    PDF »
TMEM16A Protein: A New Identity for Ca2+-Dependent Cl- Channels.
L. Ferrera, A. Caputo, and L. J. V. Galietta (2010)
Physiology 25, 357-363
   Abstract »    Full Text »    PDF »
Allergic airway inflammation induces a pro-secretory epithelial ion transport phenotype in mice.
P. Anagnostopoulou, L. Dai, J. Schatterny, S. Hirtz, J. Duerr, and M. A. Mall (2010)
Eur. Respir. J. 36, 1436-1447
   Abstract »    Full Text »    PDF »
Inhibition of Ca2+-activated Cl- channels by gallotannins as a possible molecular basis for health benefits of red wine and green tea.
W. Namkung, J. R. Thiagarajah, P.-W. Phuan, and A. S. Verkman (2010)
FASEB J 24, 4178-4186
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882