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Science 322 (5901): 590-594

Copyright © 2008 by the American Association for the Advancement of Science

TMEM16A, A Membrane Protein Associated with Calcium-Dependent Chloride Channel Activity

Antonella Caputo,1 Emanuela Caci,1 Loretta Ferrera,1 Nicoletta Pedemonte,2 Cristina Barsanti,1 Elvira Sondo,1 Ulrich Pfeffer,3 Roberto Ravazzolo,1 Olga Zegarra-Moran,1 Luis J. V. Galietta1,2*

Abstract: Calcium-dependent chloride channels are required for normal electrolyte and fluid secretion, olfactory perception, and neuronal and smooth muscle excitability. The molecular identity of these membrane proteins is still unclear. Treatment of bronchial epithelial cells with interleukin-4 (IL-4) causes increased calcium-dependent chloride channel activity, presumably by regulating expression of the corresponding genes. We performed a global gene expression analysis to identify membrane proteins that are regulated by IL-4. Transfection of epithelial cells with specific small interfering RNA against each of these proteins shows that TMEM16A, a member of a family of putative plasma membrane proteins with unknown function, is associated with calcium-dependent chloride current, as measured with halide-sensitive fluorescent proteins, short-circuit current, and patch-clamp techniques. Our results indicate that TMEM16A is an intrinsic constituent of the calcium-dependent chloride channel. Identification of a previously unknown family of membrane proteins associated with chloride channel function will improve our understanding of chloride transport physiopathology and allow for the development of pharmacological tools useful for basic research and drug development.

1 Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova 16148, Italy.
2 Centro di Biotecnologie Avanzate, Genova 16132, Italy.
3 National Cancer Research Institute, Genova 16132, Italy.

* To whom correspondence should be addressed. E-mail: galietta{at}unige.it

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
Tmem16A Encodes the Ca2+-activated Cl- Channel in Mouse Submandibular Salivary Gland Acinar Cells.
V. G. Romanenko, M. A. Catalan, D. A. Brown, I. Putzier, H. C. Hartzell, A. D. Marmorstein, M. Gonzalez-Begne, J. R. Rock, B. D. Harfe, and J. E. Melvin (2010)
J. Biol. Chem. 285, 12990-13001
   Abstract »    Full Text »    PDF »
Evidence for intestinal chloride secretion.
M. Murek, S. Kopic, and J. Geibel (2010)
Exp Physiol 95, 471-478
   Abstract »    Full Text »    PDF »
Molecular components of signal amplification in olfactory sensory cilia.
T. Hengl, H. Kaneko, K. Dauner, K. Vocke, S. Frings, and F. Mohrlen (2010)
PNAS 107, 6052-6057
   Abstract »    Full Text »    PDF »
Expression and Function of Epithelial Anoctamins.
R. Schreiber, I. Uliyakina, P. Kongsuphol, R. Warth, M. Mirza, J. R. Martins, and K. Kunzelmann (2010)
J. Biol. Chem. 285, 7838-7845
   Abstract »    Full Text »    PDF »
Tmem16b is Specifically Expressed in the Cilia of Olfactory Sensory Neurons.
S. Rasche, B. Toetter, J. Adler, A. Tschapek, J. F. Doerner, S. Kurtenbach, H. Hatt, H. Meyer, B. Warscheid, and E. M. Neuhaus (2010)
Chem Senses 35, 239-245
   Abstract »    Full Text »    PDF »
Exon-Based Clustering of Murine Breast Tumor Transcriptomes Reveals Alternative Exons Whose Expression Is Associated with Metastasis.
M. Dutertre, M. Lacroix-Triki, K. Driouch, P. de la Grange, L. Gratadou, S. Beck, S. Millevoi, J. Tazi, R. Lidereau, S. Vagner, et al. (2010)
Cancer Res. 70, 896-905
   Abstract »    Full Text »    PDF »
Mechanisms of Ca2+-stimulated fluid secretion by porcine bronchial submucosal gland serous acinar cells.
R. J. Lee and J. K. Foskett (2010)
Am J Physiol Lung Cell Mol Physiol 298, L210-L231
   Abstract »    Full Text »    PDF »
Crofelemer, an Antisecretory Antidiarrheal Proanthocyanidin Oligomer Extracted from Croton lechleri, Targets Two Distinct Intestinal Chloride Channels.
L. Tradtrantip, W. Namkung, and A. S. Verkman (2010)
Mol. Pharmacol. 77, 69-78
   Abstract »    Full Text »    PDF »
Studies on expression and function of the TMEM16A calcium-activated chloride channel.
F. Huang, J. R. Rock, B. D. Harfe, T. Cheng, X. Huang, Y. N. Jan, and L. Y. Jan (2009)
PNAS 106, 21413-21418
   Abstract »    Full Text »    PDF »
Regulation of TMEM16A Chloride Channel Properties by Alternative Splicing.
L. Ferrera, A. Caputo, I. Ubby, E. Bussani, O. Zegarra-Moran, R. Ravazzolo, F. Pagani, and L. J. V. Galietta (2009)
J. Biol. Chem. 284, 33360-33368
   Abstract »    Full Text »    PDF »
Complex Phosphatase Regulation of Ca2+-activated Cl- Currents in Pulmonary Arterial Smooth Muscle Cells.
R. Ayon, W. Sones, A. S. Forrest, M. Wiwchar, M. L. Valencik, A. R. Sanguinetti, B. A. Perrino, I. A. Greenwood, and N. Leblanc (2009)
J. Biol. Chem. 284, 32507-32521
   Abstract »    Full Text »    PDF »
Brn3a and Nurr1 Mediate a Gene Regulatory Pathway for Habenula Development.
L. A. Quina, S. Wang, L. Ng, and E. E. Turner (2009)
J. Neurosci. 29, 14309-14322
   Abstract »    Full Text »    PDF »

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