Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 322 (5905): 1243-1247

Copyright © 2008 by the American Association for the Advancement of Science

Regulation of Microtubule Dynamics by Reaction Cascades Around Chromosomes

Chaitanya A. Athale, Ana Dinarina, Maria Mora-Coral, Céline Pugieux, Francois Nedelec, Eric Karsenti*

Abstract: During spindle assembly, chromosomes generate gradients of microtubule stabilization through a reaction-diffusion process, but how this is achieved is not well understood. We measured the spatial distribution of microtubule aster asymmetry around chromosomes by incubating centrosomes and micropatterned chromatin patches in frog egg extracts. We then screened for microtubule stabilization gradient shapes that would generate such spatial distributions with the use of computer simulations. Only a long-range, sharply decaying microtubule stabilization gradient could generate aster asymmetries fitting the experimental data. We propose a reaction-diffusion model that combines the chromosome generated Ran–guanosine triphosphate–Importin reaction network to a secondary phosphorylation network as a potential mechanism for the generation of such gradients.

Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, Heidelberg, Germany.

* To whom correspondence should be addressed. E-mail: karsenti{at}embl.de


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Geometry sensing by self-organized protein patterns.
J. Schweizer, M. Loose, M. Bonny, K. Kruse, I. Monch, and P. Schwille (2012)
PNAS 109, 15283-15288
   Abstract »    Full Text »    PDF »
Speed, dissipation, and error in kinetic proofreading.
A. Murugan, D. A. Huse, and S. Leibler (2012)
PNAS 109, 12034-12039
   Abstract »    Full Text »    PDF »
A journey from reductionist to systemic cell biology aboard the schooner Tara.
E. Karsenti (2012)
Mol. Biol. Cell 23, 2403-2406
   Abstract »    Full Text »    PDF »
Phenotypic analysis of misato function reveals roles of noncentrosomal microtubules in Drosophila spindle formation.
V. Mottier-Pavie, G. Cenci, F. Verni, M. Gatti, and S. Bonaccorsi (2011)
J. Cell Sci. 124, 706-717
   Abstract »    Full Text »    PDF »
A computational model predicts Xenopus meiotic spindle organization.
R. Loughlin, R. Heald, and F. Nedelec (2010)
J. Cell Biol. 191, 1239-1249
   Abstract »    Full Text »    PDF »
Kinetochore-microtubule interactions: steps towards bi-orientation.
T. U. Tanaka (2010)
EMBO J. 29, 4070-4082
   Abstract »    Full Text »    PDF »
Towards a quantitative understanding of mitotic spindle assembly and mechanics.
A. Mogilner and E. Craig (2010)
J. Cell Sci. 123, 3435-3445
   Abstract »    Full Text »    PDF »
Fast Microtubule Dynamics in Meiotic Spindles Measured by Single Molecule Imaging: Evidence That the Spindle Environment Does Not Stabilize Microtubules.
D. J. Needleman, A. Groen, R. Ohi, T. Maresca, L. Mirny, and T. Mitchison (2010)
Mol. Biol. Cell 21, 323-333
   Abstract »    Full Text »    PDF »
Relative contributions of chromatin and kinetochores to mitotic spindle assembly.
C. B. O'Connell, J. Loncarek, P. Kalab, and A. Khodjakov (2009)
J. Cell Biol. 187, 43-51
   Abstract »    Full Text »    PDF »
Computer simulations predict that chromosome movements and rotations accelerate mitotic spindle assembly without compromising accuracy.
R. Paul, R. Wollman, W. T. Silkworth, I. K. Nardi, D. Cimini, and A. Mogilner (2009)
PNAS 106, 15708-15713
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882