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Science 322 (5906): 1380-1384

Copyright © 2008 by the American Association for the Advancement of Science

A Genetic Framework for the Control of Cell Division and Differentiation in the Root Meristem

Raffaele Dello Ioio,1 Kinu Nakamura,2* Laila Moubayidin,1* Serena Perilli,1* Masatoshi Taniguchi,2 Miyo T. Morita,3 Takashi Aoyama,2 Paolo Costantino,1 Sabrina Sabatini1{dagger}

Abstract: Plant growth and development are sustained by meristems. Meristem activity is controlled by auxin and cytokinin, two hormones whose interactions in determining a specific developmental output are still poorly understood. By means of a comprehensive genetic and molecular analysis in Arabidopsis, we show that a primary cytokinin-response transcription factor, ARR1, activates the gene SHY2/IAA3 (SHY2), a repressor of auxin signaling that negatively regulates the PIN auxin transport facilitator genes: thereby, cytokinin causes auxin redistribution, prompting cell differentiation. Conversely, auxin mediates degradation of the SHY2 protein, sustaining PIN activities and cell division. Thus, the cell differentiation and division balance necessary for controlling root meristem size and root growth is the result of the interaction between cytokinin and auxin through a simple regulatory circuit converging on the SHY2 gene.

1 Dipartimento di Genetica e Biologia Molecolare, Laboratorio di Genomica e proteomica funzionale dei sistemi modello (FGPL), Università La Sapienza - Piazzale Aldo Moro 5, 00185 Rome, Italy.
2 Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
3 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0101, Japan.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: sabrina.sabatini{at}uniroma1.it

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