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Science 323 (5921): 1610-1614

Copyright © 2009 by the American Association for the Advancement of Science

Variants of the Antibody Herceptin That Interact with HER2 and VEGF at the Antigen Binding Site

Jenny Bostrom,1,2 Shang-Fan Yu,3 David Kan,3 Brent A. Appleton,1 Chingwei V. Lee,1,2 Karen Billeci,4 Wenyan Man,1 Franklin Peale,5 Sarajane Ross,3 Christian Wiesmann,1 Germaine Fuh1,2*

Abstract: The interface between antibody and antigen is often depicted as a lock and key, suggesting that an antibody surface can accommodate only one antigen. Here, we describe an antibody with an antigen binding site that binds two distinct proteins with high affinity. We isolated a variant of Herceptin, a therapeutic monoclonal antibody that binds the human epidermal growth factor receptor 2 (HER2), on the basis of its ability to simultaneously interact with vascular endothelial growth factor (VEGF). Crystallographic and mutagenesis studies revealed that distinct amino acids of this antibody, called bH1, engage HER2 and VEGF energetically, but there is extensive overlap between the antibody surface areas contacting the two antigens. An affinity-improved version of bH1 inhibits both HER2- and VEGF-mediated cell proliferation in vitro and tumor progression in mouse models. Such "two-in-one" antibodies challenge the monoclonal antibody paradigm of one binding site, one antigen. They could also provide new opportunities for antibody-based therapy.

1 Department of Protein Engineering, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
2 Department of Antibody Engineering, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
3 Department of Translational Oncology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
4 Department of Assay and Automation Technology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.
5 Department of Pathology, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.

* To whom correspondence should be addressed. E-mail: gml{at}gene.com


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