Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

Science 323 (5921): 1614-1617

Copyright © 2009 by the American Association for the Advancement of Science

Ankyrin-G Promotes Cyclic Nucleotide–Gated Channel Transport to Rod Photoreceptor Sensory Cilia

Krishnakumar Kizhatil,1 Sheila A. Baker,2 Vadim Y. Arshavsky,2 Vann Bennett1*

Abstract: Cyclic nucleotide–gated (CNG) channels localize exclusively to the plasma membrane of photosensitive outer segments of rod photoreceptors where they generate the electrical response to light. Here, we report the finding that targeting of CNG channels to the rod outer segment required their interaction with ankyrin-G. Ankyrin-G localized exclusively to rod outer segments, coimmunoprecipitated with the CNG channel, and bound to the C-terminal domain of the channel β1 subunit. Ankyrin-G depletion in neonatal mouse retinas markedly reduced CNG channel expression. Transgenic expression of CNG channel β-subunit mutants in Xenopus rods showed that ankyrin-G binding was necessary and sufficient for targeting of the β1 subunit to outer segments. Thus, ankyrin-G is required for transport of CNG channels to the plasma membrane of rod outer segments.

1 Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
2 Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, USA.

* To whom correspondence should be addressed. E-mail: benne012{at}

Interaction of 4.1G and cGMP-gated channels in rod photoreceptor outer segments.
C. L. Cheng and R. S. Molday (2013)
J. Cell Sci. 126, 5725-5734
   Abstract »    Full Text »    PDF »
Genetic Studies of Spectrin in the Larval Fat Body of Drosophila melanogaster: Evidence for a Novel Lipid Uptake Apparatus.
B. Diaconeasa, G. H. Mazock, A. P. Mahowald, and R. R. Dubreuil (2013)
Genetics 195, 871-881
   Abstract »    Full Text »    PDF »
Cell- and subunit-specific mechanisms of CNG channel ciliary trafficking and localization in C. elegans.
M. Wojtyniak, A. G. Brear, D. M. O'Halloran, and P. Sengupta (2013)
J. Cell Sci. 126, 4381-4395
   Abstract »    Full Text »    PDF »
The giant spectrin {beta}V couples the molecular motors to phototransduction and Usher syndrome type I proteins along their trafficking route.
S. Papal, M. Cortese, K. Legendre, N. Sorusch, J. Dragavon, I. Sahly, S. Shorte, U. Wolfrum, C. Petit, and A. El-Amraoui (2013)
Hum. Mol. Genet. 22, 3773-3788
   Abstract »    Full Text »    PDF »
A Single Divergent Exon Inhibits Ankyrin-B Association with the Plasma Membrane.
M. He, W.-C. Tseng, and V. Bennett (2013)
J. Biol. Chem. 288, 14769-14779
   Abstract »    Full Text »    PDF »
Two structural components in CNGA3 support regulation of cone CNG channels by phosphoinositides.
G. Dai, C. Peng, C. Liu, and M. D. Varnum (2013)
J. Gen. Physiol. 141, 413-430
   Abstract »    Full Text »    PDF »
Cysteine 70 of Ankyrin-G Is S-Palmitoylated and Is Required for Function of Ankyrin-G in Membrane Domain Assembly.
M. He, P. Jenkins, and V. Bennett (2012)
J. Biol. Chem. 287, 43995-44005
   Abstract »    Full Text »    PDF »
The Arf GAP ASAP1 provides a platform to regulate Arf4- and Rab11-Rab8-mediated ciliary receptor targeting.
J. Wang, Y. Morita, J. Mazelova, and D. Deretic (2012)
EMBO J. 31, 4057-4071
   Abstract »    Full Text »    PDF »
Scoring a backstage pass: Mechanisms of ciliogenesis and ciliary access.
F. R. Garcia-Gonzalo and J. F. Reiter (2012)
J. Cell Biol. 197, 697-709
   Abstract »    Full Text »    PDF »
In Situ Visualization of Protein Interactions in Sensory Neurons: Glutamic Acid-Rich Proteins (GARPs) Play Differential Roles for Photoreceptor Outer Segment Scaffolding.
L. M. Ritter, N. Khattree, B. Tam, O. L. Moritz, F. Schmitz, and A. F. X. Goldberg (2011)
J. Neurosci. 31, 11231-11243
   Abstract »    Full Text »    PDF »
Membrane Anchoring Subunits Specify Selective Regulation of RGS9{middle dot}G{beta}5 GAP Complex in Photoreceptor Neurons.
Y. Cao, A. V. Kolesnikov, I. Masuho, V. J. Kefalov, and K. A. Martemyanov (2010)
J. Neurosci. 30, 13784-13793
   Abstract »    Full Text »    PDF »
Review series: The cell biology of vision.
C.-H. Sung and J.-Z. Chuang (2010)
J. Cell Biol. 190, 953-963
   Abstract »    Full Text »    PDF »
Transgene Rescue Identifies an Essential Function for Drosophila {beta} Spectrin in the Nervous System and a Selective Requirement for Ankyrin-2-binding Activity.
G. H. Mazock, A. Das, C. Base, and R. R. Dubreuil (2010)
Mol. Biol. Cell 21, 2860-2868
   Abstract »    Full Text »    PDF »
Molecular mechanisms of protein and lipid targeting to ciliary membranes.
B. T. Emmer, D. Maric, and D. M. Engman (2010)
J. Cell Sci. 123, 529-536
   Abstract »    Full Text »    PDF »
Impaired cone function and cone degeneration resulting from CNGB3 deficiency: down-regulation of CNGA3 biosynthesis as a potential mechanism.
X.-Q. Ding, C. S. Harry, Y. Umino, A. V. Matveev, S. J. Fliesler, and R. B. Barlow (2009)
Hum. Mol. Genet. 18, 4770-4780
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882